Articles tagged "Tolerance"

2017 American Transplant Congress
Dual Costimulatory Blockades Failed to Achieve Transplant Tolerance Despite Induction of Mixed Chimerism in NHPs.
T. Oura,¹ K. Hotta,¹ I. Rosales,² A. Dehnadi,¹ J. Lei,¹ J. James Markmann,¹ M. Matsunami,¹ J. Paster,¹ I. Hanekamp,¹ K. Pruner,¹ K. Kawai,¹ D. Ndishabandi,² R.-N. Smith,² B. Cosimi,¹ T. Kawai.¹
¹Department of Surgery, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA; ²Department of Pathology, Massachusetts General Hospital, Boston, MA
Background: Our previous studies showed that substantial deletion of CD8+ memory T cells (TMEM) was necessary to induce mixed chimerism (MC) and allograft tolerance when…
2017 American Transplant Congress
Suppression of T Cell Proliferation by CpG-Activated B Cells Depends on Granzyme-B and Cell-Cell Contact, but Not on IL-10.
L. Kojima,¹ S. Kimura,^1,2 K. Lee,¹ C. Schuetz,¹ L. Washburn,¹ K. Deng,¹ H. Tector,¹ B. Amundsen,^1,2 D. Ndishabandi,^1,2 A. Markmann,¹ M. Markmann-Porch,¹ T. Oura,¹ A. Alessandrini,^1,2 J. Kim,¹ H. Yeh,^1,2 J. Markmann.^1,2
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA; ²Transplantation Unit, Department of Surgery, Massachusetts General Hospital, Boston, MA
Background: Our previous studies document the potent capacity of regulatory B cells (Bregs) to mitigate allograft immunity. However, we have been largely unsuccessful in demonstrating…
2017 American Transplant Congress
The Transcription Factor Batf and IRF1 Controls IL-10/IL-27 Expression in Dendritic Cells and Transplant Tolerance Induction.
G. Gu, H. Hang, Q. Xia.
Department of Hepatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China
Objective: To explore the effect of Batf and IRF1 on IL-10/IL-27 expression in DCs during transplant tolerance induction.Methods: Allogeneic heart transplants were performed between WT…
2017 American Transplant Congress
In Depth Characterization of Ex Vivo Expanded Polyclonal Regulatory T Cells Used in a Phase I Clinical Trial Following Kidney Transplantation.
J. Heinrichs,¹ J. Mathew,^1,2 I. Konieczna,¹ J. He,¹ X. Haung,¹ A. LeFever,¹ J. Leventhal.¹
¹Surgery, Feinberg School of Medicine, Northwestern University, Chicago, IL; ²Microbiology and Immunology, Northwestern University, Chicago, IL
Initial clinical trials have investigated the safety of regulatory T cells (Tregs) as a therapy during aberrant immune activation, like graft-versus-host disease (GVHD) following stem…
2016 American Transplant Congress
Developing a PCR-Based System to Access the Correlation of Local Chimerism and Fate of the Vascularized Composite Allotransplant.
X.-T. Huang,¹ H.-Y. Cheng,¹ C.-F. Lin,¹ S.-S. Qiu,² L.-Y. Shih,¹ F.-C. Wei.^1,2,3
¹Center for Vascularized Composite Allotransplantation, Chang Gung Memorial Hospital, Gueishan, Taoyuan, Taiwan; ²Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Gueishan, Taoyuan, Taiwan; ³College of Medicine, Chang Gung University, Gueishan, Taoyuan, Taiwan.
It is established that development of mixed chimerism following allotransplantation is associated with induction of tolerance to allotransplants. Although chimerism in peripheral blood can be…
2016 American Transplant Congress
The Function of CD8⁺TCR^– Facilitating Cells and Regulatory T Cells Is Impaired in Flt3-Ligand Knockout Mice.
Y. Huang, H. Xu, T. Miller, Y. Wen, S. Ildstad.
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY.
Graft CD8+TCR- facilitating cells (FC) enhance engraftment of hematopoietic stem cells (HSC) in allogeneic mouse recipients without causing graft-versus-host disease. FC induce antigen-specific CD4+CD25+FoxP3+ regulatory…
2016 American Transplant Congress
Impact of Mixed Xenogeneic Porcine Hematopoietic Chimerism on Human NK Cell Recognition in a Humanized Mouse Model.
H. Li,¹ P. Vishwasrao,^1,2 M. Holzl,¹ S. Chen,¹ G. Choi,^2,3 G. Zhao,¹ M. Sykes.¹
¹Columbia Center for Translational Immunology, Columbia University, New York, NY; ²Department of Hematology, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands; ³Department of Hematology, University Medical Center Groningen, Groningen, Netherlands.
Xenotransplantation is a potential solution for the shortage of organs for transplantation. However, potent xenograft rejection responses present a formidable barrier. Mixed xenogeneic hematopoietic chimerism…
2016 American Transplant Congress
Enhanced IL-6 Production Impedes Transplant Tolerance Induction in Lupus-Prone Mice.
B. Stocks, C. Wilson, M. Seto, A. Marshall, D. Moore.
Pediatrics and Pathology, Microbiology, and Immunology, Vanderbilt University, Nashville, TN.
Significance: Autoimmunity is a significant barrier to transplantation. Based on previous studies, it is apparent that there are separate genetic regions that control susceptibility to…
2016 American Transplant Congress
Modification of DC Phenotype and Function via Targeted Porous Silicon Nanoparticles.
S. Stead,¹ S. McInnes,² S. Kireta,³ P. Rose,¹ D. Rojas-Canales,³ S. Jesudason,³ S. Grey,⁴ R. Carroll,³ N. Voelcker,² P. Coates.^1,3
¹School of Medicine, University of Adelaide, Adelaide, Australia; ²Future Industries Institute, University of South Australia, Adelaide, Australia; ³CNARTS, Royal Adelaide Hospital, Adealide, Australia; ⁴Transplantation Immunology, Garvan Institute of Medical Research, Sydney, Australia.
Aims: Dendritic cells (DC) are the most potent antigen-presenting cell and are fundamental in the establishment of transplant tolerance. Targeting DC via the DC-SIGN receptor…
2016 American Transplant Congress
Membrane Proteome Profiling Reveals Molecular Changes Associated with Donor-Specific Tolerance to Vascularized Composite Allotransplants.
H.-Y. Cheng,¹ C.-L. Han,² F.-W. Jiang,² L.-Y. Shih,¹ C.-F. Lin,¹ Y.-J. Chen,³ F.-C. Wei.^1,4
¹Center for Vascularized Composite Allotransplantation, Chang Gung Memorial Hospital, Gueishan, Taoyuan, Taiwan; ²Master Program for Pharmacogenomics and Pharmacoproteomics, Taipei Medical University, Taipei, Taiwan; ³Institute of Chemistry, Academia Sinica, Taipei, Taiwan; ⁴College of Medicine, Chang Gung University, Gueishan, Taoyuan, Taiwan.
Background: although donor-specific tolerance to vascularized composite allotransplants (VCAs) has been successfully induced with different regimes, molecular changes associated with tolerance are not well understood.Materials…