Articles tagged "Tolerance"

2017 American Transplant Congress
Suppression of T Cell Proliferation by CpG-Activated B Cells Depends on Granzyme-B and Cell-Cell Contact, but Not on IL-10.
L. Kojima,¹ S. Kimura,^1,2 K. Lee,¹ C. Schuetz,¹ L. Washburn,¹ K. Deng,¹ H. Tector,¹ B. Amundsen,^1,2 D. Ndishabandi,^1,2 A. Markmann,¹ M. Markmann-Porch,¹ T. Oura,¹ A. Alessandrini,^1,2 J. Kim,¹ H. Yeh,^1,2 J. Markmann.^1,2
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA; ²Transplantation Unit, Department of Surgery, Massachusetts General Hospital, Boston, MA
Background: Our previous studies document the potent capacity of regulatory B cells (Bregs) to mitigate allograft immunity. However, we have been largely unsuccessful in demonstrating…
2017 American Transplant Congress
The Transcription Factor Batf and IRF1 Controls IL-10/IL-27 Expression in Dendritic Cells and Transplant Tolerance Induction.
G. Gu, H. Hang, Q. Xia.
Department of Hepatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China
Objective: To explore the effect of Batf and IRF1 on IL-10/IL-27 expression in DCs during transplant tolerance induction.Methods: Allogeneic heart transplants were performed between WT…
2017 American Transplant Congress
In Depth Characterization of Ex Vivo Expanded Polyclonal Regulatory T Cells Used in a Phase I Clinical Trial Following Kidney Transplantation.
J. Heinrichs,¹ J. Mathew,^1,2 I. Konieczna,¹ J. He,¹ X. Haung,¹ A. LeFever,¹ J. Leventhal.¹
¹Surgery, Feinberg School of Medicine, Northwestern University, Chicago, IL; ²Microbiology and Immunology, Northwestern University, Chicago, IL
Initial clinical trials have investigated the safety of regulatory T cells (Tregs) as a therapy during aberrant immune activation, like graft-versus-host disease (GVHD) following stem…
2017 American Transplant Congress
Applicability and Safety of a Regulatory T Cell Therapy in Adult Liver Transplantation: The “ThRIL” Phase I First-in-Human Trial.
G. Whitehouse, N. Safinia, S. Thirkell, L. Fry, N. Grageda, M. Martinez-Llordella, R. Lechler, N. Heaton, G. Lombardi, A. Sanchez-Fueyo.
MRC Centre for Transplantation, King's College London, London, United Kingdom
Aim“ThRIL” is a multiple site, open-label, uncontrolled, phase I clinical trial assessing the safety and applicability of a CD4+CD25+FOXP3+ regulatory T cell (Treg) immunotherapy in…
2017 American Transplant Congress
Autologous Expanded Tregs and Porcine Hematopoietic Cells to Prolong Xenogeneic Pig Skin Graft Acceptance in Baboons.
J. Stern,¹ S. Kofman,¹ P. Alonso-Guallart,¹ E. Berglund,¹ M. Danton,¹ J. Weiner,¹ D. Ayares,² R. Hawley,¹ K. Yamada,¹ D. Sachs,¹ M. Sykes,¹ A. Griesemer.¹
¹Columbia Center for Translational Immunology, Columbia University, New York, NY; ²Revivicor, Blacksburg, VA
Background: Mixed chimerism (MC) has been shown to induce tolerance to allogeneic organs and has shown promise for application to xenotransplants, although long-term tolerance has…
2017 American Transplant Congress
Persistent Donor Antigen Leads to Dysfunctional CD4⁺Memory T Cell Development.
M. Miller,¹ Y. Wang,¹ L. Chen,¹ Y. Lei,¹ M. Daniels,^2,3 E. Watkins,⁴ C. Mora Solano,² C. McIntosh,¹ N. Isaad,⁵ A. Chong,² M.-L. Alegre.¹
¹Medicine, University of Chicago, Chicago, IL; ²Surgery, University of Chicago, Chicago, IL; ³Chicago State University, Chicago, IL; ⁴Institute for Molecular Engineering, University of Chicago, Chicago, IL; ⁵Committee on Cancer Biology, University of Chicago, Chicago, IL
Tolerance to alloantigens is characterized by low alloreactive T cell responses upon restimulation with donor antigens, a state that can result from deletion of donor-specific…
2017 American Transplant Congress
Two Months Delayed Induction of Mixed Chimerism After Heart and Kidney Cotransplantation in Nonhuman Primates.
K. Huh,¹ W. Sommer,¹ K. Robinson,¹ X. Wu,¹ J. Paster,¹ I. Hanekamp,¹ A. Dehnadi,¹ T. Kawai,^1,2 R. Smith,³ R. Colvin,^1,3 G. Benichou,¹ J. Madsen.^1,4
¹Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital, Boston, MA; ²Department of Surgery, Massachusetts General Hospital, Boston, MA; ³Department of Pathology, Massachusetts General Hospital, Boston, MA; ⁴Division of Cardiac Surgery, Department of Surgery, Massachusetts General Hospital, Boston, MA
Background We have previously achieved transplant tolerance in cynomolgus recipients cotransplanted with heart and kidney by treating recipients with standard triple drug immunosuppression for 4…
2017 American Transplant Congress
CD19⁺CD38^hiCD24^hi Immature Transitional B Cells in PBMCs of Tolerant Kidney Recipients of Facilitating Cell-Enriched Hematopoietic Stem Cells and Renal Allografts.
Y. Wen,¹ A. Merchak,¹ Y. Huang,¹ H. Xu,¹ L. Kahn,¹ J. Leventhal,² S. Ildstad.¹
¹Institute for Cellular Therapeutics, University of Louisville, Louisville, KY; ²Comprehensive Transplant Center, Department of Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL
CD19+CD38hiCD24hi immature transitional B cells may represent a biomarker for transplantation tolerance in kidney transplant (KTx) recipients (Cherukuri A, et al. J Am Soc Nephrol.…
2017 American Transplant Congress
Induction of Sensitization and Resistance to Transplant Tolerance by Allogeneic Pregnancy.
A. Suah,¹ J. Young,¹ S. Hsu-Wei Khiew,¹ Q. Wang,¹ D. Yin,¹ M.-L. Alegre,² A. Chong.¹
¹Department of Surgery, University of Chicago, Chicago, IL; ²Department of Medicine, University of Chicago, Chicago, IL
Background: Clinical data suggest allogeneic pregnancy is a sensitizing event; yet, recent data from mouse models suggest it induces fetal-specific regulatory T cell (Treg) expansion…
2017 American Transplant Congress
B Cells in Liver Transplant Tolerance.
M. Morita,¹ G. Asonye,¹ N. Gupta,¹ J. Fung,² C. Miller,³ S. Qian,^1,3 L. Lu.^1,3
¹Immunology, Cleveland Clinic, Cleveland; ²University of Chicago, Chicago, MI; ³General Surgery, Cleveland Clinic, Cleveland, OH
In mice liver transplantation, the liver allografts can be accepted across fully mismatched MHC without immunosuppression. After transplantation, T cells infiltrate into the graft in…