Articles tagged "Tolerance"

2018 American Transplant Congress
AMD3100 (Plerixafor) as a Single-Dose Stem Cell Mobilizing Agent in Vascularized Composite Tissue Allograft (VCA) Transplantation in a Canine Model
B. Swearingen,^1,2 S. Graves,^2,3 R. Storb,^2,3 D. Mathes.^1,2
¹Surgery; Division of Plastic and Reconstructive Surgery, University of Colorado School of Medicine, Aurora, CO; ²Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA; ³Medicine, University of Washington Medical Center, Seattle, WA.
PURPOSE:Vascularized Composite Allograft (VCA) transplantation is a clinical reality but limited by chronic immunosuppression and rejection. Current clinical tolerance protocols rely on recipient conditioning and…
2018 American Transplant Congress
Donor Bone Marrow CXCR4+ Foxp3+ Treg Cells Are Essential for Costimulation Blockade-Induced Long-Term Survival of Murine Limb Transplants
L. Wang,¹ Z. Wang,¹ R. Han,¹ G. Ge,¹ L. Levin,² M. Levine,² W. Hancock.^1,2
¹CHOP, Philadelphia; ²UPenn, Philadelphia.
Vascularized composite allotransplantation (VCA) would likely be more widely performed if maintenance immunosuppression was not essential for graft acceptance. Accordingly, we used murine models of…
2018 American Transplant Congress
Engineering Regulatory T Cells with Interleukin (IL-)2 Nanogel for Improved Alloimmune Suppression
S. Eskandari,¹ M. Bandeira Melo,² J. Assaker,¹ A. Mansouri,¹ S. Cai,¹ B. Al Dulaijan,¹ M. Mohamed,¹ D. Irvine,² J. Azzi.¹
¹Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School, Boston, MA; ²Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA.
Clinical trials of adoptively transferred regulatory T cells (TRegs) have been hindered by the short lifespan of these transferred TRegs. Systemic injection of low-dose interleukin…
2018 American Transplant Congress
Lymph Node Reprogramming: Microparticle Delivery of Immune Signals to Induce Tolerance and Prolong Allograft Survival
Y. Xiong,¹ L. Tostanoski,² C. Jewell,² J. Bromberg.¹
¹UMB, MD; ²UMCP, MD.
Introduction: Microparticle (MP) loaded with peptide antigen plus other immune signals and delivered directly into lymph nodes (LN) can result in either pro-inflammatory or anti-inflammatory…
2018 American Transplant Congress
TGF-b Plus Rapamycin Induce Foxp3 and Promote iTreg Development and Suppressive Function, Leading to Long-Term Allograft Survival
L. Wang, A. Samanta, R. Han, N. Zhou, W. Hancock.
Path & Lab Med, CHOP & UPenn, Philadelphia, PA.
Given the major roadblocks to development of Treg-based cell therapy, pharmacologic approaches to expand endogenous Tregs warrant attention, including approaches with more sustained effects than…
2018 American Transplant Congress
In Vitro α-Galactosylceramide Stimulation Expands CD4⁺CD25⁺Foxp3⁻ Regulatory T Cell Precursors in Murine Thymocytes
H. Katsumata,^1,2 M. Ikemiyagi,¹ T. Kanzawa,¹ H. Fukuda,¹ R. Ishii,¹ K. Saiga,^1,3 M. Okumi,¹ Y. Ishii,⁴ K. Tanabe.¹
¹Urology, Tokyo Women's Medical University, Tokyo, Japan; ²Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan; ³Urology, Jyoban Hospital of Tokiwa Foundation, Fukushima, Japan; ⁴Laboratory for Immunotherapy, Center for Integrative Medical Sciences (IMS) RIKEN, Yokohama, Japan.
Background: We previously established mixed chimerism after invariant natural killer T-cell stimulation by administration of liposomal α-galactosylceramide (lipo-αGC) and costimulatory blockade, leading to regulatory T…
2018 American Transplant Congress
Influence of Dendritic Cell Subsets on Enhancing Oral Tolerance in Cardiac Transplantation Model
G. Gu, T. Lu, N. Xu, Q. Xia.
Department of Liver Surgery, Renji Hospital, Affiliated by Shanghai Jiaotong University Medical School, Shanghai, China.
Oral tolerance has been implicated in the protection of transplant rejection in the animal models. Antigen presenting cells (APCs) are responsible for sampling their environment…
2018 American Transplant Congress
Prevention and Reversal of Diabetes through Restoration of B and T Cell Regulation and Immune Homeostasis
K. Brayman,¹ P. Chhabra,¹ C. Wilson,² A. Marshall,² D. Moore.²
¹Surgery, University of Virginia, Charlottesville, VA; ²Pediatric Endocrinology, Vanderbilt University, Nashville, TN.
Goal: To determine the mechanisms by which IgM restores immune homeostasis in new-onset diabetic NOD mice.Methods: 1) 5wks old non-obese diabetic (NOD) mice received mouse…
2018 American Transplant Congress
Ex Vivo-Expanded Human CD19⁺CD73^–CD25⁺CD71⁺TIM-1⁺ B Regulatory Cells Can Prolong Allograft Survival in a Humanized Mouse Model of Skin Transplantation and Are Dependent on TIM-1-Mediated Regulation of STAT3 Signaling
S. Shankar,¹ J. Stolp,^1,2 S. Juvet,^1,3 J. Hester,¹ K. Wood.¹
¹Nuffield Department of Surgical Sciences, University of Oxford, Oxford, United Kingdom; ²Garvan Institute of Medical Research, Sydney, Australia; ³Toronto General Hospital Research Institute, Toronto, Canada.
The importance of B regulatory cells (Breg) in both health and disease has rapidly become evident over the last decade. Breg are able to control…
2018 American Transplant Congress
Long-Term Nonhuman Primate Renal Allograft Survival without Ongoing Immunosuppression in Recipients of Delayed Donor Bone Marrow Transplantation
K. Hotta, T. Oura, A. Dehnadi, M. Matsunami, I. Rosales, R. Smith, R. Colvin, A. Cosimi, T. Kawai.
Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA.
Background: We have previously reported induction of renal allograft tolerance in nonhuman primates following an initial post-transplant period of conventional immunosuppression (IS) using a nonmyeloablative…