Articles tagged "Tolerance"

2020 American Transplant Congress
Establishment of Spontaneous Tolerance of Kidney Allografts is Dependent on Mitigation of Ischemia-Reperfusion Injury via the BLTA-HVEM Pathway
Q. Yuan¹, S. Hong¹, E. Szuter¹, Y. Zhao¹, E. Acheampong¹, I. Rosales¹, M. Kronenberg², P. Russell¹, J. Madsen¹, R. Colvin¹, A. Alessandrini¹
¹Massachusetts General Hospital, Boston, MA, ²La Jolla Institute for Allergy & Immunology, La Jolla, CA
*Purpose: Kidney allografts transplanted across certain mouse strain combinations (e.g. DBA/2 to B6) develop tolerance while other combinations do not (e.g. B6 to DBA/2). We…
2020 American Transplant Congress
Granulocyte-Colony Stimulating Factor (G-CSF) and Granulocyte-Macrophage CSF (GM-CSF) Administration Enriches for Highly Suppressive CD4⁺CD45RA^–Foxp3^hi Cells in Leukapheresis Products of Rhesus Monkeys
K. Sasaki, Y. Wang, L. Lu, J. Hughes, V. Vujevich, A. W. Thomson, M. B. Ezzelarab
Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA
*Purpose: In humans, CD4+CD45RA-Foxp3hi T cells comprise a regulatory T cell (Treg) subset with highly suppressive function. We have previously shown that granulocyte-colony stimulating factor…
2020 American Transplant Congress
Detrimental Effects of Donor Brain Death on Tolerance Induction May be Eliminated by Delaying Mixed Chimerism in Non-Human Primates
J. M. O¹, W. Sommer², K. Ahrens¹, P. Patel¹, D. Becerra¹, J. Morrissette¹, T. Costa¹, K. Pruner¹, J. Paster¹, A. Bean¹, A. Dehnadi¹, I. Hanekamp¹, I. Rosales¹, R. Smith¹, R. Colvin¹, G. Benichou¹, J. Allan³, T. Kawai¹, J. Madsen⁴
¹Center for Transplantation Sciences, MGH, Boston, MA, ²Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany, ³Division of Thoracic Surgery, MGH, Boston, MA, ⁴Division of Cardiac Surgery, MGH, Boston, MA
*Purpose: Induction of tolerance to heart allografts in non-human primates is possible with kidney cotransplantation and mixed chimerism-based induction therapy. However, donor brain death abrogates…
2020 American Transplant Congress
Autophagy-Lysosome Inhibitor Chloroquine Prevents CTLA-4 Degradation of T Cells and Attenuates Acute Rejection in Murine Skin and Heart Transplantation
J. Wu
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
*Purpose: The immune checkpoint cytotoxic T lymphocyte antigen-4 (CTLA-4), which was induced upon T cell activation but degraded quickly, has been targeted in the clinical…
2020 American Transplant Congress
Orthogonal IL-2 Cytokine-Receptor Pair Capable of Selective Expansion of Regulatory T Cells Facilitates Alloengraftment
T. Hirai¹, F. Simonetta², L. L. Su³, L. Picton³, J. Baker², J. Lin⁴, P. Li⁴, W. J. Leonard⁴, K. C. Garcia³, R. S. Negrin²
¹Department of Urology, Tokyo Women's Medical University, Tokyo, Japan, ²Division of Blood and Marrow Transplantation, Stanford University, Stanford, CA, ³Departments of Molecular and Cellular Physiology and Structural Biology, Stanford University, Stanford, CA, ⁴Laboratory of Molecular Immunology, National Institutes of Health, Bethesda, MD
*Purpose: Adoptive transfer of regulatory T cells (Tregs) has been shown to improve alloengraftment in animal models. However, ex vivo Treg expansion for the purpose…
2020 American Transplant Congress
Autologous Expanded Tregs Promote Bone Marrow Engraftment and Donor Kidney Survival in Non-Human Primate Chimerism Model
K. Bruestle¹, P. Alonso-Guallart¹, H. Sakai¹, F. Fredriksson¹, B. Piegari¹, E. Lopes¹, G. Pierre¹, A. Shou¹, D. Ekanayake-Alper¹, J. Weiner¹, A. Iuga², S. M. Coley², M. Sykes¹, A. Griesemer¹
¹Columbia Center for Translational Immunology, Columbia University, New York City, NY, ²Department of Pathology and Cell Biology, Columbia University, New York City, NY
*Purpose: Mixed chimerism (MC) induced with non-myeloablative conditioning in monkeys and humans induces tolerance to 70% of kidney grafts transplanted at the time of BMT.…
2020 American Transplant Congress
ESAT-6, A Novel Protein Originally Produced by M. Tuberculosis, Inhibits Central Memory CD8+ T Cell Generation and Allograft Rejection
F. Qiu, S. Ye, H. Liu, Z. Dai
Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou, China
*Purpose: Allospecific memory T cells pose a threat to long-term allograft survival since they are somewhat resistant to immunosuppression. The objective of this study is…
2020 American Transplant Congress
HLA Molecular Mismatch Analysis in Recipients of Kidney Plus Hematopoietic Stem/Facilitating Cell Transplant Tolerance Induction Protocol
C. Garcia-Sanchez¹, V. Kosmoliaptsis², H. Copley², J. Leventhal¹, S. Ildstad³
¹NU, Chicago, IL, ²Cambridge, Cambridge, United Kingdom, ³U of Louisville, Lousville, KY
*Purpose: To evaluate the potential role of HLA molecular mismatch (MM) analysis in predicting outcomes in a cohort of HLA-mismatched, related and unrelated recipients of…
2019 American Transplant Congress
Role of MHC Class II in B Cell and Regulatory T Cell Development
S. K. Germana, Z. Wang, C. A. Huang, L. Kojima, C. G. Rickert, J. F. Markmann, C. LeGuern
Surgery, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA
*Purpose: Therapies involving CD4 regulatory T cells (Tregs) are promising approaches to mitigate graft rejection and autoimmunity. Understanding of the activation mechanisms that initiate Treg…
2019 American Transplant Congress
Promotion of Chimerism by Lymphohematopoietic Graft-versus-Host Responses in Patients Receiving Intestinal Transplantation
J. Fu¹, J. Zuber¹, B. Shonts¹, A. Obradovic¹, K. Frangaj¹, E. E. Waffarn¹, S. Lau¹, T. M. Savage¹, S. Yang¹, K. Rogers¹, P. Satwani², S. Ho¹, Y. Shen³, A. Griesemer⁴, M. Martinez², T. Kato⁴, M. Sykes¹
¹Columbia Center for Translational Immunology, Department of Medicine, Columbia University, New York, NY, ²Department of Pediatrics, Columbia University, New York, NY, ³Department of Systems Biology, Columbia University, New York, NY, ⁴Department of Surgery, Columbia University, New York, NY
*Purpose: Long-term multilineage chimerism often appears in blood following intestinal transplantation (ITx), usually without graft-versus-host disease (GVHD). Patients with donor T cell macrochimerism (≥4%) in…