Articles tagged "Tolerance"

2020 American Transplant Congress
TGF-b Induced Myeloid-Derived Suppressor Cells Prevent Transplatation Rejection by Promoting Immune Tolerance
P. Cao, Z. Sun, J. Zhang, F. Zhang, W. Wang
Department of Urology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China
*Purpose: Myeloid-derived suppressor cells (MDSCs) are a class of heterogeneous myeloid cells with immunosuppressive function. An effective method to produce MDSCs with better capacity and…
2020 American Transplant Congress
IL-10 Signaling in T Cells is Essential for Induction of Transplant Tolerance
M. Iglesias Lozano, D. Bibicheff, M. Chicco, G. Brandacher, G. Raimondi
Plastic Surgery, Johns Hopkins University, Baltimore, MD
*Purpose: Induction of long term transplant survival by “costimulation blockade” (CoB) is achieved through deletion of abortively activated alloreactive T cells and generation of graft…
2020 American Transplant Congress
Plasma Microbiome Profiles of Liver Transplant Patients with Operational Tolerance
C. Kuscu¹, J. Pierre², I. Lies¹, C. Kuscu¹, A. Bajwa¹, A. Wolen¹, J. Eason¹, B. Ramaswami³, D. Maluf¹, G. Mazariegos³, G. Chalasani³, V. Mas¹
¹James D. Eason Transplant Institute, Department of Surgery, UTHSC, Memphis, TN, ²Department of Pediatrics, UTHSC, Memphis, TN, ³Thomas E. Starzl Transplantation Institute, Children's Hospital of Pittsburgh, UPMC, Pittsburgh, PA
*Purpose: Microbiome has emerged as a key modulator of organ homeostasis and immune system function with altered microbiota and microbiome - host interactions contributing to…
2020 American Transplant Congress
Prognostic Value of Dd-cfDNA Assay in Acute Renal Rejection Therapy: A Prospective Cohort Study
L. Guo, R. Wang, J. Lv, J. Shen, J. Wu, J. Chen
Kidney Disease Center, First Affiliated Hospital of Zhejiang University, Hangzhou, China
*Purpose: Donor-derived cell free DNA (dd-cfDNA) is a promising biomarker for monitoring allograft status. However, whether it is capable of reflecting real time anti-rejection treatment…
2020 American Transplant Congress
Single Cell RNA Sequencing Analysis of 3 Human Living-Donor Liver Transplant Allograft Biopsies after Infusion of Donor-Derived Regulatory Dendritic Cells
L. M. Tran¹, T. Tabib², A. F. Zahorchak¹, R. Lafyatis², M. A. Styn¹, A. Humar¹, F. G. Lakkis¹, D. M. Metes¹, A. W. Thomson¹
¹Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, ²Department of Medicine and Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA
*Purpose: Single-cell RNA sequencing is an emerging, powerful genomic technology that can describe heterogenous cellular populations of whole solid organs with high resolution. We report…
2020 American Transplant Congress
Pregnancy Alloimmunization Increases the Clonality of the Endogenous Treg Repertoire
K. Krupp, E. Lewis, R. Xu, P. Porrett
University of Pennsylvania, Philadelphia, PA
*Purpose: Pregnancy is a common immune sensitizing event, which creates an access barrier to organ transplantation and can impact transplant outcomes. While most research has…
2020 American Transplant Congress
Recruitment of Regulatory T Cells Promotes Donor-Specific Tolerance in Vascularized Composite Allotransplantation
J. D. Fisher¹, W. Zhang², S. C. Balmert³, A. M. Aral¹, Y. Kulahci⁴, A. P. Acharya⁴, J. Li², H. R. Turnquist⁵, A. W. Thomson⁵, M. G. Solari², V. S. Gorantla⁶, S. R. Little⁴
¹Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, ²Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, ³Department of Dermatology, University of Pittsburgh, Pittsburgh, PA, ⁴Department of Chemical Engineering, University of Pittsburgh, Pittsburgh, PA, ⁵Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA, ⁶Department of Bioengineering, Wake Forest University, Winston-Salem, NC
*Purpose: Vascularized composite allotransplantation (VCA) encompasses face and limb transplantation, but as with organ transplantation, it requires lifelong regimens of immunosuppressive drugs to prevent rejection.*Methods:…
2020 American Transplant Congress
Establishment of Spontaneous Tolerance of Kidney Allografts is Dependent on Mitigation of Ischemia-Reperfusion Injury via the BLTA-HVEM Pathway
Q. Yuan¹, S. Hong¹, E. Szuter¹, Y. Zhao¹, E. Acheampong¹, I. Rosales¹, M. Kronenberg², P. Russell¹, J. Madsen¹, R. Colvin¹, A. Alessandrini¹
¹Massachusetts General Hospital, Boston, MA, ²La Jolla Institute for Allergy & Immunology, La Jolla, CA
*Purpose: Kidney allografts transplanted across certain mouse strain combinations (e.g. DBA/2 to B6) develop tolerance while other combinations do not (e.g. B6 to DBA/2). We…
2020 American Transplant Congress
Granulocyte-Colony Stimulating Factor (G-CSF) and Granulocyte-Macrophage CSF (GM-CSF) Administration Enriches for Highly Suppressive CD4⁺CD45RA^–Foxp3^hi Cells in Leukapheresis Products of Rhesus Monkeys
K. Sasaki, Y. Wang, L. Lu, J. Hughes, V. Vujevich, A. W. Thomson, M. B. Ezzelarab
Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA
*Purpose: In humans, CD4+CD45RA-Foxp3hi T cells comprise a regulatory T cell (Treg) subset with highly suppressive function. We have previously shown that granulocyte-colony stimulating factor…
2020 American Transplant Congress
Detrimental Effects of Donor Brain Death on Tolerance Induction May be Eliminated by Delaying Mixed Chimerism in Non-Human Primates
J. M. O¹, W. Sommer², K. Ahrens¹, P. Patel¹, D. Becerra¹, J. Morrissette¹, T. Costa¹, K. Pruner¹, J. Paster¹, A. Bean¹, A. Dehnadi¹, I. Hanekamp¹, I. Rosales¹, R. Smith¹, R. Colvin¹, G. Benichou¹, J. Allan³, T. Kawai¹, J. Madsen⁴
¹Center for Transplantation Sciences, MGH, Boston, MA, ²Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany, ³Division of Thoracic Surgery, MGH, Boston, MA, ⁴Division of Cardiac Surgery, MGH, Boston, MA
*Purpose: Induction of tolerance to heart allografts in non-human primates is possible with kidney cotransplantation and mixed chimerism-based induction therapy. However, donor brain death abrogates…