Articles tagged "Tolerance"

2020 American Transplant Congress
Development and Cell Surface Testing of a Novel Nanofiber Technology for Targeted Delivery of Therapeutic Regulatory T Cells
J. M. Mathew¹, S. Lee², Y. Sambandam¹, L. A. Fraser², T. D. Clemons², A. Kolberg², J. He¹, L. C. Palmer², M. T. McClendon², Z. J. Zhang¹, J. R. Leventhal¹, S. I. Stupp², L. Gallon¹
¹Surgery -Transplant, Northwestern University, Chicago, IL, ²Simpson Querrey Institute, Northwestern University, Chicago, IL
*Purpose: Regulatory CD4+CD25+FOXP3+ T cells (Tregs) have been shown to induce tolerance in transplantation models, and to be increased in their numbers in tolerant human…
2020 American Transplant Congress
Donor-Derived DCREG Infusion Prolongs Kidney Allograft Survival in Rhesus Monkeys Treated with Co-Stimulation Blockade and Calcineurin Inhibition
Y. Wang, S. Kazuki, L. Lu, A. Zahorchak, H. Dai, A. Ganoza, M. Wijkstrom, A. Humar, M. Ezzelarab, A. Thomson
Thomas E. Starzl Transplantation Institute, Pittsburgh, PA
*Purpose: We have shown previously that pre-transplantation (Tx) infusion of donor-derived regulatory dendritic cells (DCreg) safely prolongs MHC-mismatched renal allograft survival and regulates donor-reactive T…
2020 American Transplant Congress
Unexpected Regulation of Foxp3+ Treg Production, Stability and Function by the Nuclear Co-Repressor, Sin3A
L. M. Christensen¹, T. Akimova², R. Han¹, L. Wang¹, A. Samanta¹, W. W. Hancock²
¹Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, ²Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA
*Purpose: Foxp3+ T-regulatory (Treg) cells are crucial to immune homeostasis and self-tolerance, but the contributions to Tregs of the 4 large, multiprotein nuclear complexes (Sin3,…
2020 American Transplant Congress
Human, Monkey Transplant Tolerance and IL35 “Exokine
W. J. Burlingham¹, E. Jankowska-Gan², D. Lema², J. H. Fechner², J. A. Sullivan², D. B. Kaufman²
¹University of Wisconsin, Madison, WI, ²Surgery, University of Wisconsin, Madison, WI
*Purpose: Studies in B6 (H2b) mice tolerized to CBA (H2k) have shown that induced allospecific Tregs make 2 very different forms of immunoregulatory cytokines: 1)…
2020 American Transplant Congress
Tracking Tregs in a Non-Human Primate Model with Expanded Autologous Treg Infusions In Vivo
K. Bruestle¹, B. Piegari¹, H. Sakai¹, F. Fredriksson¹, D. Ekanayake-Alper¹, S. Robertson², D. Hajosi², J. Weiner¹, A. Iuga³, S. M. Coley³, M. Sykes¹, A. Griesemer¹
¹Columbia Center for Translational Immunology, Columbia University, New York City, NY, ²Institute for Comparative Medicine, Columbia University, New York City, NY, ³Department of Pathology and Cell Biology, Columbia University, New York City, NY
*Purpose: Regulatory Tcell (Treg) therapy is a promising tool for tolerance induction in solid organ transplantation. Despite encouraging results in humans and monkeys, questions about…
2020 American Transplant Congress
Early Post-Transplant Infusion of Ex Vivo-Expanded Autologous Polyclonal Regulatory T Cells (Treg) Prolongs Kidney Allograft Survival in Non-Lymphodepleted, CTLA4Ig-Treated Rhesus Monkeys
K. Sasaki, Y. Wang, R. Nakano, L. Lu, J. Hughes, V. Vujevich, A. J. Ganoza, M. Wijkstrom, A. Humar, A. W. Thomson, M. B. Ezzelarab
Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA
*Purpose: Rodent studies have demonstrated the utility of regulatory T cell (Treg) infusion for the promotion of tolerance to organ allografts. Here, we evaluated the…
2020 American Transplant Congress
Infectious Tolerance Depends Upon the ‘Exokine’ IL35
W. J. Burlingham¹, J. A. Sullivan¹, Y. Tomita², E. Jankowska-Gan¹, D. Lema¹, S. Hong³, D. A. Vignali⁴
¹Surgery, University of Wisconsin, Madison, WI, ²Surgery, Tokai University, Tokyo, Japan, ³Pharmacy, University of Wisconsin, Madison, WI, ⁴Immunology, University of Pittsburgh, Pittsburgh, PA
*Purpose: Interleukin-35 (IL35) is an immunosuppressive cytokine composed of Ebi3 and p35 subunits. Yet the form(s) that IL35 assumes and its role in infectious tolerance,…
2020 American Transplant Congress
Establishing an Induction Protocol for Long-Term Multilineage Mixed Chimerism in Rhesus Macaques
K. Bruestle¹, H. Sakai¹, F. Fredriksson¹, B. Piegari¹, D. Ekanayake-Alper¹, A. McLuckie², R. Ober², J. Weiner¹, A. Iuga³, S. M. Coley³, M. Sykes¹, A. Griesemer¹
¹Columbia Center for Translational Immunology, Columbia University, New York City, NY, ²Institute for Comparative Medicine, Columbia University, New York City, NY, ³Department of Pathology and Cell Biology, Columbia University, New York City, NY
*Purpose: Mixed chimerism (MC) induction via non-myeloablative conditioning and bone marrow transplantation (BMT) induces tolerance to solid organs. Our standard MC induction protocol in cynomologous…
2020 American Transplant Congress
Gal9/Tim-3 Pathway was Required for Endometrial Stem Cells to Induce Peripheral Tolerance of Cardiac Allograft
Y. Zhao¹, Y. Hu¹, D. Yu¹, X. Li¹, W. Jin¹, Y. Qin¹, D. Kong¹, H. Wang¹, A. Alessandrini², H. Wang¹
¹Tianjin Medical University General Hospital, Tianjin, China, ²Massachusetts General Hospital, Boston, MA
*Purpose: The existing immunosuppressants seem to have little effect on Cardiac Allograft Vasculopathy but with obvious side effects. ERCs (Endometrial stem Cells), which are mesenchymal-like…
2020 American Transplant Congress
Treg-Lymphatic Interfaces: Unique Molecular Structures for Cell-Cell Interactions During Migration
C. M. Paluskievicz, W. Piao, Y. Xiong, J. S. Bromberg
U of Maryland, Baltimore, MD
*Purpose: To promote transplant tolerance, T regulatory cells (Treg) must enter the afferent lymphatic vasculature by migrating across lymphatic endothelial cells (LECs), and then home…