Articles tagged "Sensitization"

2021 American Transplant Congress
Measuring the Impact of Targeting FcRn-Mediated IgG Recycling on Donor-Specific Alloantibodies in a Sensitized NHP Model
M. Manook¹, W. Flores², R. Schmitz¹, Z. Fitch¹, J. Yoon¹, Y. Bae¹, B. I. Shaw¹, M. Harnois³, S. Permar³, A. Kirk¹, D. Magnani², J. Kwun¹, S. Knechtle¹
¹Duke Transplant Center, Duke University Medical Center, Durham, NC, ²Massbiologics, University of Massachusetts Medical School, Boston, MA, ³Human Vaccine Institute, Duke University Medical Center, Durham, NC
*Purpose: In transplantation, plasmapheresis and IVIg provide the mainstay of treatment directed at reducing or removing circulating donor specific antibody (DSA), yet both have limitations.…
2021 American Transplant Congress
Acoustofluidic Device to Remove Donor Specific Antibody in a Sensitized Animal Model
J. Kwun¹, E. David², Y. Gu³, Z. Ma³, M. Kuchibhatla⁴, G. Arepally⁵, T. J. Huang³, E. T. Chambers²
¹Surgery, Duke University, Durham, NC, ²Pediatrics, Duke University, Durham, NC, ³Biomedical Engineering, Duke University, Durham, NC, ⁴Biostatistics and Bioinformatics, Duke University, Durham, NC, ⁵Internal Medicine, Duke University, Durham, NC
*Purpose: Current therapies for antibody-mediated rejection are neither donor-specific nor are they tailored toward children, resulting in premature allograft loss and increased mortality. Apheresis involves…
2021 American Transplant Congress
Low Immunogenic Donors Improve Kidney Transplant Survival in Sensitized Recipients
D. Bekbolsynov¹, R. Green², B. Mierzejewska¹, M. Rees¹, S. Stepkowski¹
¹University of Toledo, Toledo, OH, ²Bowling Green State University, Bowling Green, OH
*Purpose: Our previous results showed that low immunogenicity (IM) donors improved kidney allograft survival in non-sensitized recipients. Herein, we examined how the level of peak…
2021 American Transplant Congress
Prospective Intensive Monitoring for Anamnestic DSA Responses Allows Near Elimination of Early Clinical AMR
M. McGowan¹, A. Bickenbach¹, A. R. Shields², R. R. Alloway¹, P. Brailey¹, E. Portwood¹, C. Alquist¹, A. Christianson¹, B. Abu Jawdeh¹, M. Cuffy¹, J. Kremer², S. Bumb¹, A. Govil¹, M. Anand¹, T. Kaur¹, E. S. Woodle¹
¹U Cincinnati, Cincinnati, OH, ²Christ Hospital, Cincinnati, OH
*Purpose: Anamnestic donor specific antibody (aDSA) responses can result in severe, graft threatening antibody-mediated rejection (AMR). We hypothesized that intensive DSA monitoring (IDM) for aDSA…
2021 American Transplant Congress
Natural Sensitization by Pregnancy, Testing Desensitization Strategies in Female NHPs Across Repeat Mismatches
M. Manook, J. Yoon, Z. Fitch, R. Schmitz, A. M. Jackson, J. Kwun, S. Knechtle
Duke Transplant Center, Duke University Medical Center, Durham, NC
*Purpose: Women are disproportionately represented in the highly sensitized patient cohort, and HLA-incompatible transplantation is undertaken predominantly in females. However, nonhuman primate (NHP) models to…
2021 American Transplant Congress
Impact of Allosensitization on Xenotransplantation
D. Olaso¹, M. Manook¹, J. Yoon¹, Y. Bae¹, A. Barbas¹, A. Adams², S. Knechtle¹, J. Kwun¹
¹Department of Surgery, Duke Transplantation Center, Durham, NC, ²Department of Surgery, University of Minnesota, Minneapolis, MN
*Purpose: Sensitization to human leukocyte antigens (HLA) due to prior exposure to foreign antigens through events such as blood transfusions, previous kidney transplant, or pregnancy…
2021 American Transplant Congress
Successful Prophylaxis of Antibody-Mediated Rejection by Downregulation of C5 Expression via RNA Interference in a Rat Kidney Transplant Model
H. Ishigooka¹, S. Motoi², T. Yamakawa¹, C. Matsui², Y. Suzuki³, R. Ishii¹, K. Saiga¹, D. Tokita¹, T. Imai², K. Tanabe¹
¹Urology, Tokyo Women's Medical University, Tokyo, Japan, ²KAN Research Institute, Inc., Kobe, Japan, ³Tsukuba Research Laboratories, Eisai, Co., Ltd., Ibaraki, Japan
*Purpose: Antibody-mediated rejection (AMR) is a major contributor towards poor prognosis in kidney transplantation. Circulating donor-specific antibodies (DSAs) cause AMR with complement activation. This study…
2021 American Transplant Congress
Quantifying Hidden Sensitization: HLA-reactive Memory B Cells in the Spleens of Sensitized Women
J. T. Killian¹, J. A. Houp¹, A. Nellore², R. G. King³, P. M. Porrett¹, F. E. Lund³
¹Department of Surgery, University of Alabama at Birmingham School of Medicine, Birmingham, AL, ²Department of Medicine, University of Alabama at Birmingham School of Medicine, Birmingham, AL, ³Department of Microbiology, University of Alabama at Birmingham School of Medicine, Birmingham, AL
*Purpose: Measurement of circulating anti-HLA antibody (anti-HLA-Ab) in transplant candidates and recipients is required for successful organ transplantation. However, serum Ab measurement alone likely underestimates…
2021 American Transplant Congress
Anti-HLA-DP Antibodies Positive – A Retrospective Review of Outcomes in Renal Transplantation- Single Centre Experience
O. Piscoran¹, J. Worthington², R. Dhillon¹, T. Augustine¹, M. Picton¹, M. Morton¹, S. Bhutani¹
¹Urology, Renal and Transplantation, Manchester University NHS Foundation Trust, Manchester, United Kingdom, ²Transplantation Laboratory, Manchester University NHS Foundation Trust, Manchester, United Kingdom
*Purpose: Data on the role of HLA-DP antibodies on graft outcome is not precise. Studies suggested that the presence of donor-directed HLA-DP antibodies correlate with…
2021 American Transplant Congress
Anti-pig IgE and IgA Antibodies in Sera of (i) Naïve Primates and (ii) Non-human Primates Sensitized to Pig Xenografts
H. Hara, Q. Li, H. Iwase, T. Yamamoto, D. Ayares, D. K. Cooper
University of Alabama at Birmingham, Birmingham, AL
*Purpose: Anti-pig IgM and IgG antibodies in primates play an important role in xenograft rejection. The possible roles of anti-pig IgE and IgA have not…