Articles tagged "Primates"

2015 American Transplant Congress
CD28 Saturation by FR104, a Non-Activating CD28 Antagonist Fab in a Non-Human Primate Cardiac Allograft Model
W. Sun,¹ A. Azimzadeh,¹ G. Braileanu,¹ T. Zhang,¹ D. Amoako,¹ L. Burdorf,¹ B. Vanhove,² R. Pierson III.
¹Dept of Surgery, University of Maryland School of Medicine, Baltimore, MD; ²INSERM U643, Nantes, France.
Background: Targeting CD28 costimulation pathway with a selective antagonist is a promising approach in tolerance induction. The extent to which novel therapeutic antibodies reach their…
2015 American Transplant Congress
Generation, Cryopreservation, Function and In Vivo Fate of Ex-Vivo Expanded Cynomolgus
H. Guo,¹ H. Zhang,¹ L. Lu,¹ M. Ezzelarab,¹ A. Thomson.^1,2
¹Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA; ²Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA.
Background:Foxp3+ regulatory T cells (Treg) function to promote immune tolerance and prevent immunopathogenesis. Preclinical studies have demonstrated that adoptive transfer of Treg prevents rejection of…
2015 American Transplant Congress
Donor Specific Expansion of Regulatory T-Cells Was Associated With Allograft Tolerance Induced By Transient Mixed Chimerism in Nonhuman Primates
K. Hotta, A. Aoyama, T. Ohura, M. Tonsho, Y. Yamada, J. Allan, J. Madsen, B. Cosimi, G. Benichou, T. Kawai.
Transplant Center, Massachusetts General Hospital, Boston, MA.
Background: We have previously reported successful induction of solid organ allograft tolerance in nonhuman primates (NHP) via a mixed chimerism approach. Since these recipients achieved…
2015 American Transplant Congress
Selective CD28 Blockade With or Without CD40-CD154 Blockade Promotes Cardiac Allograft Protection in Non-Human Primates
T. Zhang,¹ A. Kronfli,¹ B. Gheorghe,¹ L. Burdorf,¹ E. Rybak,¹ D. Parsell,¹ D. Harris,¹ E. Sievert,¹ S. Dahi,¹ N. Kubicki,¹ J. Whoddal,¹ M. Uluer,¹ X. Cheng,¹ E. Schwartz,¹ K. Quinn,¹ B. Vanhove,² A. Azimzadeh,¹ R. Pierson III.¹
¹Surgery, University of Maryland School of Medicine, Baltimore, MD; ²INSERM, U643, Nants, France.
Aim: Selective targeting CD28:B7 costimulation without interfering with CTLA-4-dependent immune regulation is theoretically an attractive approach to modulate pathogenic T cell-dependent immunity. We previously reported…
2015 American Transplant Congress
CD28-Directed Therapy Prolongs Survival in a Non-Human Primate Kidney Transplant Model
L. Higginbotham,¹ D. Mathews,¹ C. Breeden,¹ W. Wakwe,¹ C. Larsen,¹ S. Nadler,² A. Adams.¹
¹Emory Transplant Center, Emory University School of Medicine, Atlanta; ²Bristol-Myers Squibb, Princeton.
Introduction. Costimulation blockade using belatacept has the potential to improve long-term renal function and reduce cardiovascular risk by avoiding calcineurin inhibitors, yet higher rates and…
2015 American Transplant Congress
Selective Blockade of the CD28/B7/CTLA4 Pathway With Monovalent Anti-CD28 Antibodies Versus Targeting of B7 With CTLA4-Ig, in Non-Human Primate Kidney Allograft
S. Ville,¹ N. Poirier,^1,2 B. Vanhove,^1,2 G. Blancho.¹
¹INSERM, UMR1064, ITUN, Nantes, France; ²Efﬁmune SAS, Nantes, France.
B7 molecules on antigen-presenting cells interact with CD28 and CTLA4 on T lymphocytes to control TCR-mediated signals. Whereas it is highly efficient to block T…
2015 American Transplant Congress
Impact of Human Mutant TGF-β1/Fc Protein On Memory and Regulatory CD4+T Cells Following Lymphodepletion in Rhesus Monkeys
H. Guo,¹ L. Lu,¹ R. Wang,² H. Abdulkerim,² R. Keith,² A. Thomson,¹ M. Ezzelarab.¹
¹Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA; ²MassBiologics, University of Massachusetts Medical School, Boston, MA.
Background:Tolerance to allografts is essential to avoid chronic immunosuppression and to promote long term graft function. Transforming growth factor- β plays a key role in…
2015 American Transplant Congress
Immunosuppression With Anti-CD40 mAb and Rapamycin for Islet and Kidney Co-Transplantation in Nonhuman Primates
T. Oura, K. Hotta, J. Lei, J. Markmann, A. Dehnadi, G. Benichou, B. Cosimi, T. Kawai.
Transplant Center, Massachusetts General Hospital, Boston, MA.
Background: For patients with end-stage renal disease secondary to type 1 diabetes mellitus, islet and kidney co-transplantation can be an ideal transplant option. However, a…
2015 American Transplant Congress
Anti-CD122 + Costimulation Blockade Prolongs Allograft Survival
D. Mathews,¹ L. Higginbotham,¹ Y. Dong,¹ C. Breeden,¹ C. Larsen,¹ J. Tso,² A. Adams.¹
¹Emory Transplant Center, Emory University, Atlanta, GA; ²JN Bioscience, Mountanview, CA.
Introduction: Effective T cell activation and effector function requires (1) cognate antigen recognition (2) appropriate costimulation and (3) successful cytokine signaling. Recently approved strategies to…
2015 American Transplant Congress
Heart En Bloc Thymus Transplantation Permits Long-Term, Acute Rejection-Free Cardiac Allograft Survival in Nonhuman Primates (NHPs)
M. Tonsho,¹ P. Spencer,¹ T. Millington,¹ A. Muniappan,¹ A. Tena,² R. Smith,³ R. Colvin,³ J. Allan,^1,2 J. Madsen.^1,2,4
¹MGH Transplant Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA; ²Transplantation Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA; ³Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA; ⁴Cardiac Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA.
BACKGROUNDThe role of vascularized thymus on tolerance induction has been well studied in swine recipients of kidney allografts, but not in NHP recipients of heart…