Articles tagged "Preclinical trails"

2019 American Transplant Congress
Complement Blockade in Kidney Recipients Prevents Delayed Graft Function and Antibody-Mediated Rejection in Non-Human Primates
M. Eerhart¹, J. Reyes¹, C. Blanton¹, J. Danobeitia¹, P. Chlebeck¹, L. Zitur¹, J. Coonen¹, S. Capuano¹, A. D’Alessandro¹, J. Torrealba², W. Zhong¹, Y. Yankol¹, L. Fernandez¹
¹University of Wisconsin, Madison, WI, ²UT Southwestern, Dallas, TX
*Purpose: Ischemia-reperfusion injury (IRI) leading to inflammation, complement activation, cytokine release and acute kidney injury has been implicated in the mechanism for delayed graft function…
2019 American Transplant Congress
Achieving Robust Targeting of Antibody-Conjugated Nanoparticles Delivered during Ex Vivo Machine Perfusion of Human Kidneys
J. R. DiRito¹, S. Hosgood¹, M. L. Nicholson¹, G. Tietjen²
¹Surgery, University of Cambridge, Cambridge, United Kingdom, ²Surgery, Yale School of Medicine, New Haven, CT
*Purpose: In this study we seek to improve targeted nanoparticle (NP) delivery during ex-vivo normothermic machine perfusion through fibrinolysis for more homogenous treatment of human…
2019 American Transplant Congress
Oral Preconditioning of Donors after Brain Death with Calcineurin Inhibitors vs. Inhibitors of Mammalian Target for Rapamycin in Pig Kidney Transplantation
S. Abbasi Dezfouli¹, M. Golriz¹, O. Ghamarnejad¹, E. Khajeh¹, S. Mohammadi¹, M. Nikdad¹, A. Majlesara¹, M. Sabagh¹, P. Fathi¹, S. Shafiei¹, M. Kentar², A. Younsi², C. Eckert³, T. Poth³, A. Mehrabi¹, A. Nickkholgh¹
¹Department of General, Visceral, and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany, ²Department of Neurosurgery, University of Heidelberg, Heidelberg, Germany, ³Department of Pathology, University of Heidelberg, Heidelberg, Germany
*Purpose: The systemic inflammatory cascade triggered in donors after brain death (DBD) enhances the ischemia-reperfusion injury (IRI) after organ transplantation. Data regarding donor preconditioning with…
2019 American Transplant Congress
The Number of Donor-Specific IL-21 Producing Cells before and after Transplantation Predicts Kidney Graft Rejection
N. M. van Besouw¹, L. Yan¹, R. de Kuiper¹, M. Klepper¹, D. Reijerkerk¹, M. Dieterich¹, D. L. Roelen², F. H. Claas², M. C. Clahsen-van Groningen³, D. A. Hesselink¹, C. C. Baan¹
¹Internal Medicine - Nephrology & Transplantation, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Netherlands, ²Immunohematology and Blood Transfusion, Leiden University Medical Centre, Leiden, Netherlands, ³Pathology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Netherlands
*Purpose: Interleukin (IL)-21 supports induction and expansion of CD8+ T cells, and can also regulate the differentiation of B cells into antibody-producing plasma cells. We…
2019 American Transplant Congress
Anti- CD154 / CD40 Costimulation Blockade is Superior to Tacrolimus in Prolonging Survival in Pig-to-Nonhuman Primate Renal Xenotransplantation
B. P. Lovasik¹, A. J. Matar¹, C. Breeden¹, J. Jenkins², J. Tector³, S. C. Kim¹, A. B. Adams¹
¹Emory Transplant Center, Emory University, Atlanta, GA, ²Yerkes National Primate Center, Emory University, Atlanta, GA, ³University of Alabama at Birmingham, Birmingham, AL
*Purpose: Costimulation blockade strategies targeting the CD40/CD154 pathway are highly effective in preventing xenograft rejection in pig-to-nonhuman primate (NHP) transplantation models. The aim of this…
2019 American Transplant Congress
The Role of CD47/SIRPα and CD47/TSP-1 Pathways in Pig-to-Baboon Kidney Transplantation Using hCD47 Transgenic Kidneys
K. Takeuchi¹, Y. Ariyoshi¹, T. Pomposelli¹, L. Boyd¹, D. Ekanayake-Alper¹, S. Arn¹, R. Hawley¹, D. Ayares², M. Lorber³, M. Sykes¹, D. Sachs¹, R. Johnson⁴, K. Yamada¹
¹CCTI/Surgery, Columbia University Medical Center, New York, NY, ²Revivicor Inc., Blacksburg, VA, ³Lung BioTechnology PBC, Silver Spring, MD, ⁴Division of Renal Diseases and Hypertension, University of Colorado, Aurora, CO
*Purpose: We have recently reported that both CD80 upregulation and the degradation of SMPDL-3b on glomerular podocytes are involved in the development of post kidney…
2019 American Transplant Congress
Newly-Revealed Antibody Targets Lead to the Early Loss of Triple Knockout Pig Kidneys in a Pig to Baboon Model
Y. Ariyoshi¹, K. Takeuchi¹, T. Pomposelli¹, D. K. Ekanayake-Alper¹, C. Schuetz¹, H. Sahara¹, L. Boyd¹, E. Estime¹, S. Arn¹, D. Ayares², M. Lorber³, M. Sykes¹, D. Sachs¹, K. Yamada¹
¹CCTI/Surgery, Columbia University Medical Center, New York, NY, ²Revivicor Inc., Blacksburg, VA, ³Lung BioTechnology PBC, Silver Spring, MD
*Purpose: We have achieved >6 months survival of a life-supporting kidney with a vascularized thymus (VT) in a pig-to-baboon model. While improved survival has been…
2018 American Transplant Congress
The Preclinical Application of the Tactile Discrimination Task in Rat Models
S-.H. Wu,^1,2 C-.J. Wen,^1,2 J-.J. Huang,^1,2,3 Y-.C. Pei,^1,2,3 C-.H. Lin,^1,2,4,5 H. Cheng,¹ Z-.L. Peng,^1,4,5 H-.I. Yang,⁶ F-.C. Wei.^1,2,5
¹Center for Vascularized Composite Allotransplantation, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; ²College of Medicine, Chang Gung University, Taoyuan, Taiwan; ³Department of Physical Medicine & Rehabilitation, Taoyuan Chang Gung Memorial Hospital, Taoyuan, Taiwan; ⁴Division of Trauma and Emergency Plastic Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; ⁵Division of Plastic Reconstructive Microsurgery, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; ⁶Department of Electronic Engineering, National Taipei University of Technology, Taipei, Taiwan.
Nerve reinnervation is an important issue in the field of vascularized composite allotransplantation (VCA). However, nowadays the effective treatment for enhancing the reinnervation after the…
2018 American Transplant Congress
Tomotherapy and Donor Hematopoietic Cells for Tolerance to Kidney Transplants in Rhesus Macaques
D. Kaufman,¹ L. Haynes,¹ P. Hematti,¹ W. Burlingham,¹ L. Forest,² J. Post,¹ R. Redfield,¹ L. Fernandez,¹ A. D'Alessandro,¹ J. Haynes,¹ K. Jensen,³ S. Strober.³
¹Surgery, University of Wisconsin School of Medicine, Madison, WI; ²Surgical Sciences, University of Wisconsin School of Vet Med, Madison, WI; ³Medicine, Stanford University Medical School, Palo Alto, CA.
The goal of this project was to develop a tolerance induction protocol for MHC 1-haplotype mismatched kidney transplants (txs) in a rhesus macaque model. The…
2018 American Transplant Congress
Immunomodulation of Alloantibody Responses
C. Schuetz, M. Climov, A. Paril, Z. Wang, A. Andrews Roy, N. Navarro Alvarez, A. Matar, D. Sachs, R. Duran-Struuck, C. Huang.
Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA.
We have shown that i.v. exposure to MHC-mismatched donor PBMC using a novel, mild conditioning protocol results in B-cell tolerance with transient T-cell unresponsiveness. Here…