Articles tagged "Pig"

2019 American Transplant Congress
TMA Development in Kidney and Heart Xenografts Treated with Anti-CD40 or Anti-CD154 Antibodies
D. H. Ma¹, H. Sasaki¹, J. M. O¹, K. Ahrens¹, D. Becerra¹, C. G. Rickert¹, D. M. Detelich¹, T. Oura¹, W. Somer¹, I. Rosales², R. N. Pierson¹, J. Markmann¹, J. C. Madsen¹, T. Kawai¹
¹Surgery, MGH, Boston, MA, ²Pathology, MGH, Boston, MA
*Purpose: Genetically modified Galactose-α 1,3-galactose knockout transgenic swine expressing human CD55 (GTKO.hCD55) are resistant to hyperacute rejection of xenografts. We compare anti-CD154 and anti-CD40 antibody-based…
2018 American Transplant Congress
An Effective Tolerance Approach for Porcine Islet Xenotransplantation in Humanized Mice
F. Lee,¹ A. Dangi,¹ M. Burnette,¹ X. Zhang,¹ B. Hering,² X. Luo.¹
¹Comprehensive Transplant Center, Northwestern University, Chicago, IL; ²Diabetes/Endocrinology, Diabetes Institute, Minneapolis, MN.
Purpose: Tolerance for pig-to-human islet transplantation could render a feasible and sustainable cure for T1D. We have previously described a triple therapy (TT) approach consisting…
2018 American Transplant Congress
Genetically Modified Porcine Erythrocytes: A Cell Model for Studying Human Preformed Antibody-Dependent Xenoimmune Responses In Vitro
Z. Wang, G. Martens, J. Ladowski, M. Tector, A. Tector.
Surgery, University of Alabama at Birmingham, Birmingham, AL.
Background: Powerful gene editing technology is a promising approach to provide genetically-modified pigs as donors for clinical cell, tissue and organ xenotransplantation. Prior to starting…
2018 American Transplant Congress
Curative Correction of FAH Deficiency by Chimeric Stem Cells Transplantation
D. Joo,^1,2 E. Nelson,¹ Y. Zhang,¹ B. Amiot,¹ S. Ho,³ C. Lancto,⁴ D. Carlson,⁴ L. Queiroz,⁵ S. Nyberg.¹
¹Division of Transplant Surgery, Mayo Clinic, Rochester, MN; ²Department of Surgery, Yonsei University College of Medicine, Seoul, Korea; ³Department of Pathology, University of Michigan, Ann Arbor, MI; ⁴Recombinetics Inc, Saint Paul, MN; ⁵International Center for Biotechnology, Cooperative Resources International, Mount Horeb, WI.
Fumarylacetoacetate hydrolase (FAH) deficient pigs have been proposed as surrogate hosts for large scale in vivo expansion of normal FAH+ hepatocytes. However, immunologic rejection prevents…
2018 American Transplant Congress
Intensified Immunosuppression Improves Early Renal Allograft Function after Transplantation in a Porcine Model with Extended Asystolic Warm Ischemic Time
M. Xu,¹ X. Wang,¹ S. Garcia-Aroz,¹ B. Banan,¹ B. Rabe,¹ Z. Zhang,¹ J. Jia,¹ G. Upadhya,¹ P. Manning,² J. Gaut,³ Y. Lin,¹ W. Chapman.¹
¹Department of Surgery, Section of Abdominal Transplantation, Washington University School of Medicine, St. Louis, MO; ²Arch Oncology, St. Louis, MO; ³Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO.
PURPOSE: Extensive ischemia-reperfusion injury significantly increases the immunogenicity of renal allograft which may be associated with increased risk of acute rejection (AR). This study investigated…
2018 American Transplant Congress
A Proteomic Analysis of Kidneys Subjected to Normothermic Ex Vivo Kidney Perfusion (NEVKP) Demonstrates That Metabolism is an Important Determinant of Kidney Function Following Warm Ischemia
A. Konvalinka,^1,4 T. Tokar,² S. Reid,⁴ P. Urbanellis,⁴ M. Hamar,⁴ M. Kaths,⁴ I. Jurisica,² L. Robinson,^4,3 M. Selzner.^4,5
¹Medicine/Nephrology, University Health Network, Toronto, ON, Canada; ²Medical Biophysics/Computer Science, University of Toronto, Toronto, ON, Canada; ³Pediatrics/Nephrology, Hospital for Sick Children, Toronto, ON, Canada; ⁴Institute of Medical Science, University of Toronto, Toronto, ON, Canada; ⁵Surgery/General Surgery, University Health Network, Toronto, ON, Canada.
Kidney transplantation reduces mortality compared to staying on dialysis, incentivizing the use of marginal kidneys in face of organ shortage. These marginal kidneys after cardiocirculatory…
2018 American Transplant Congress
Subnormothermic Machine Perfusion (SNMP) with Human Hemoglobin(Hb)-Based Oxygen Carriers (HBOCs)perfusate Decreased Hepatocellular Reperfusion Injury of Donation after Cardiac Death (DCD) Donor in Pig Model
T. Shonaka,¹ N. Matsuno,¹ H. Obara,² R. Yoshikawa,² Y. Nishikawa,³ M. Gochi,¹ M. Otani,¹ H. Takahashi,¹ H. Azuma,³ H. Sakai,⁴ H. Furukawa.¹
¹Department of Surgery, Asahikawa Medical University, Asahikawa, Japan; ²Tokyo Metroporitan University, Tokyo, Japan; ³Asahikawa Medical University, Asahikawa, Japan; ⁴Nara Medical University, Nara, Japan.
Subnormothermic machine perfusion (SNMP) alleviate hepatoceller injury during organ preservation. However more oxygen is required when perfusate SNMP. Some studies demonstrated that SNMP with hemoglobin-based…
2018 American Transplant Congress
Normothermic Ex-Vivo Kidney Perfusion Restores the Genetic Profile of Marginal Kidney Grafts Subjected to Warm Ischemia
P. Urbanellis,¹ M. Hamar,¹ I. Linares,¹ D. Kollmann,¹ S. Ganesh,¹ R. John,¹ P. Yip,¹ I. Mucsi,¹ A. Ghanekar,¹ D. Bagli,² A. Konvalinka,¹ D. Grant,¹ L. Robinson,² M. Selzner.¹
¹University Health Network, Toronto, ON, Canada; ²SickKids Hospital, Toronto, ON, Canada.
Background: Increasing evidence demonstrates the superiority of normothermic ex-vivo kidney perfusion (NEVKP) over cold storage (CS) for marginal renal grafts. To account for this improvement,…
2018 American Transplant Congress
Ischemia-Free Liver Transplantation in Pigs: Ischemia Reperfusion Injury is Avoidable?
Z. Guo, X. He, Y. Tang, F. Ji, Z. Zhang, Q. Zhao, S. Huang, Z. Zhu, W. Ju, G. Chen.
Organ Transplant Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
BACKGROUND&AIMS: In organ transplantation, ischemia and reperfusion injury (IRI) is considered as an inevitable event and the major contributor of graft failure. Great efforts have…
2018 American Transplant Congress
Assessment of Bile Production and Quality during Normothermic Ex Vivo Liver Perfusion to Predict Biliary Injury
D. Kollmann,¹ I. Linares,¹ S. Ganesh,¹ R. Rosales,¹ M. Hamar,¹ P. Urbanellis,¹ A. Wiebe,¹ P. Yip,² O. Adeyi,² M. Selzner,¹ N. Selzner.¹
¹Multi Organ Transplant Program, Toronto General Hospital, Toronto, Canada; ²Laboratory Medicine and Pathobiology, University Health Network, Toronto, Canada.
Background. Liver transplantation (LT) with donation after circulatory death (DCD)-grafts is associated with higher risk for biliary strictures. We evaluated bile production and quality during…