Articles tagged "Mice"

2016 American Transplant Congress
mTORC2 Negatively Regulates Dendritic Cell (DC) Metabolism and the Allostimulatory Function of Donor DCs in Organ Transplantation.
A. Watson,^1,2 D. Raïche-Regué,¹ H. Dai,¹ K. Fabian,^3,4 G. Delgoffe,^4,5 W. Storkus,^3,4 A. Thomson.^1,2
¹Starzl Transplantation Institute, Univ. of Pittsburgh, Pittsburgh, PA; ²Pathology, Univ. of Pittsburgh, Pittsburgh, PA; ³Dermatology, Univ. of Pittsburgh, Pittsburgh, PA; ⁴Immunology, Univ. of Pittsburgh, Pittsburgh, PA; ⁵Cancer Institute, Univ. of Pittsburgh, Pittsburgh, PA.
The mechanistic target of rapamycin (mTOR) is a kinase that functions in at least two complexes: rapamycin (RAPA)-sensitive mTORC1 and RAPA-insensitive mTORC2. These complexes play…
2016 American Transplant Congress
Contributions of Naive vs. Endogenous and Induced Alloreactive Memory T Cells in Mixed Lymphocyte Reactions.
J. Lee, G. Benichou.
Surgery, MGH/Harvard Medical School, Boston, MA.
T lymphocytes isolated from naïve, unmanipulated mice, proliferate and secrete cytokines when cultured in vitro with allogeneic irradiated leukocytes. This in vitro response known as…
2016 American Transplant Congress
Short-Term Starvation Attenuates Liver Ischemia Reperfusion Injury by SIRT1-Autophagy Signaling in Mice.
J. Rao,¹ H. Zhou,¹ F. Zhang,¹ X. Wang,¹ Y. Zhai,² L. Lu.¹
¹Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, China; ²Dumont-UCLA Transplant Center, David Geffen School of Medicine at University of California-Los Angeles, Los Angeles.
Calorie restriction or starvation (fasting) has some beneficial effects on prolongation of life and increased resistance to stress. Calorie restriction has also been shown to…
2016 American Transplant Congress
Role of NRP1 in CD4+ T Cell Allo-Activation.
E. Lee, H. Kim, G. Demirci.
Department of Surgery, Boston Children's Hospital, Boston, MA.
Introduction: Neuropilin-1 (NRP1) is a transmembrane glycoprotein that functions as a co-receptor for class III/IV semaphorins, VEGF, and several other growth factors. NRP1 has been…
2015 American Transplant Congress
Recipient IL-33 Promotes Type 1 Alloimmunity and Lethal Acute GVHD Following alloHCT
B. Matta,¹ D. Reichenbach,² V. Schwarze,³ V. Tkachev,⁴ L. Mathews,¹ Q. Liu,¹ M. Warncke,⁵ J. Ferrara,⁴ R. Zeiser,³ B. Blazar,² H. Turnquist.¹
¹Surgery, Univ. of Pittsburgh, Pittsburgh, PA; ²Pediatrics, Univ. of Minnesota, Minneapolis, MN; ³Hematology and Oncology, Univ. Medical Center Freiburg, Freiburg, Germany; ⁴Pediatrics, Univ. of Michigan, Ann Arbor, MI; ⁵Novartis Institute for Biomedical Research, Basel, Switzerland.
Background: Graft-versus-host disease (GVHD) is a common complication of allogeneic (allo) hematopoietic cell transplantation (HCT) that limits its widespread application for the induction of transplant…
2015 American Transplant Congress
Mesenchymal Stem Cells Prevent Cold Ischemia-Reperfusion Injury in Lung Transplants
H. Wang,^1,2 W. Tian,¹ Y. Liu,³ X. Dai,¹ G. Li,³ T. Liu,^1,2 Z. Zhang,^1,2 C. Du.⁴
¹Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, China; ²Tianjin General Surgery Institute, Tianjin, China; ³Department of Biology, Tianjin Medical University, Tianjin, China; ⁴Department of Urologic Sciences, The University of British Columbia, Vancouver, BC, Canada.
Background: Cold ischemia-reperfusion injury (IRI) is a major cause of graft failure in lung transplantation. Despite therapeutic benefits of mesenchymal stem cells (MSCs) in attenuating…
2015 American Transplant Congress
Myeloid Cell-Specific Heme Oxygenase-1 Is Crucial for Hepatoprotection in Liver Ischemia-Reperfusion Injury
K. Nakamura,¹ M. Zhang,² B. Ke,¹ R. Busuttil,¹ J. Araujo,² J. Kupiec-Weglinski.¹
¹Surgery, Liver Transplant, University of California, Los Angeles, Los Angeles, CA; ²Medicine, Division of Cardiology, University of California, Los Angeles, Los Angeles, CA.
Background and Aim: Heme oxygenase-1 (HO-1), an anti-oxidant enzyme, is essential in tissue resistance against innate immunity driven ischemia-reperfusion injury (IRI). Our group was the…
2015 American Transplant Congress
Effects of Toll Like Receptor (TLR) Signaling on Murine Alloimmunity Are Modulated by Immune Cell Derived Complement
J. Sheen, P. Heeger.
Translational Transplant Research Center, Department of Medicine and Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY.
TLR signals prevent CD40L-blockade (MR1) induced murine transplant tolerance but mechanisms are incompletely understood. Based on previous findings that immune cell derived complement activation products…
2015 American Transplant Congress
Phenotype Characterization, Migratory Pathways, Engraftment and Safety Evaluation of Human Cord Blood Derived Ex-Vivo Created Di- Chimeric Cells in NOD SCID Mouse Model: A Preliminary Study
J. Cwykiel,¹ A. Sklodowska,¹ W. Malewski,¹ M. Cyran,¹ H. Karagoz,¹ E. Bryndza Tfaily,¹ M. Askar,² M. Siemionow.¹
¹Department of Orthopaedics, University of Illinois at Chicago, Chicago, IL; ²Transplant Center, Cleveland Clinic, Cleveland, OH.
PURPOSE: The aim of this study was to evaluate safety, survival, migration and engraftment of ex-vivo fused human cord blood derived di-chimeric cells (DCC) in…
2015 American Transplant Congress
Identification of Mouse Tolerogenic B Cells Expressing PD-L1/PD-L2 in B-1a Subset
T. Hirose,^1,2 Y. Tanaka,¹ H. Sakai,¹ Y. Sasaki,¹ H. Ohdan.¹
¹Gastroenterological and Transplant Surgery, Hiroshima University, Hiroshima, Japan; ²Renal and Genitourinary Surgery, Hokkaido University, Sapporo, Hokkaido, Japan.
Donor lymphocyte infusion (DLI) has been shown to induce donor-specific hyporesponsiveness or even immune-tolerance among alloreactive T cells in different organ transplantation models. However, the…