Articles tagged "Machine preservation"

2022 American Transplant Congress
Enhanced Delivery of Nanomedicine to Human Kidney During Ex Vivo Perfusion
C. Albert¹, L. Bracaglia¹, A. Koide², J. R. DiRito¹, T. Lysyy¹, E. Christopher¹, J. Grundler¹, J. T. Langford¹, D. Haakinson¹, J. S. Pober¹, W. M. Saltzman¹, S. Koide², G. Tietjen¹
¹Yale University, new haven, CT, ²Perlmutter Cancer Center, New York University Langone Medical Center, New York, NY
*Purpose: An efficient delivery of therapeutic agents to graft endothelial cells (ECs) prior to transplantation could render organs more resistant to injury, improving clinical outcomes…
2022 American Transplant Congress
Normothermic Ex Vivo Perfusion of Discarded Human Pancreases: A Feasibility Study to Expand the Pancreas Donor Pool
C. Parmentier, S. Ray, L. Mazilescu, M. Kawamura, Y. Noguchi, T. Goto, B. Arulratnam, S. Ganesh, M. Selzner, T. Reichman
UHN - Toronto General Hospital, Toronto, ON, Canada
*Purpose: Normothermic Ex Vivo Perfusion (NEVPP) model has been used in the clinical setting for Lung, heart, liver, and kidney. However, a system for human…
2022 American Transplant Congress
Perfusate Metabolites and Association with Pump Perfusion and Allograft Failure
R. X. Liu¹, N. Koyawala², H. T. Philbrook², I. Hall³, P. Reese⁴, S. Mohan⁵, M. Doshi⁶, C. Parikh¹
¹Johns Hopkins University School of Medicine, Baltimore, MD, ²Department of Medicine, Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, MD, ³University of Utah School of Medicine, Salt Lake City, UT, ⁴University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, ⁵University of Columbia Vagelos College of Physicians & Surgeons, New York, NY, ⁶University of Michigan, Ann Arbor, MI
*Purpose: While hypothermic machine perfusion (HMP) is associated with improved kidney graft viability, the exact biological mechanisms underlying this association are unknown. The goal of…
2022 American Transplant Congress
Fibrin Deposition in Ischemic Damaged Liver Grafts and Reconditioning with Thrombolytic Therapy During Normothermic Machine Perfusion
T. Goto, Y. Noguchi, S. Ganesh, B. Arulratnam, M. Kawamura, C. Parmentier, S. Ray, T. Reichman, N. Selzner, M. Selzner
Ajmera Transplant Program, Department of Surgery, Toronto General Hospital, University Health Network, Toronto, ON, Canada
*Purpose: Normothermic ex situ perfusion (NMP) is a novel technique for the storage and assessment of marginal livers retrieved from donors after circulatory death (DCD).…
2022 American Transplant Congress
Artificial Mitochondria Transfer to Mitigate Ischemic Damage in an In Vitro Model of Tubular Injury and in an Ex Vivo Porcine Model of DCD Renal Transplantation
A. Rossi¹, S. Sedrakyan², F. Montali³, C. Aardena⁴, N. Abraham⁵, A. Barbas⁵, L. N. Byers⁶, C. Palles⁷, A. Asthana⁶, J. Robertson⁴, L. Perin², R. Senger⁴, B. Bussolati¹, G. Orlando⁸
¹Un. of Turin, Turin, Italy, ²Children Hospital, Los Angeles, CA, ³Un. of L'Aquila, L'Aquila, Italy, ⁴VA Tech, Blacksburg, VA, ⁵Duke, Durham, NC, ⁶WFIRM, Winston Salem, NC, ⁷University of Florida, Gainesville, FL, ⁸Wake Forest Baptist, Winston-Salem, NC
*Purpose: We aimed at assessing whether artificial mitochondrial transfer (AMT) mitigates renal injury in an in vitro model of tubular damage and in an ex…
2022 American Transplant Congress
Comparison of Vasodilators in Dual Vessel Normothermic Ex Vivo Liver Machine Perfusion of Rat Liver Grafts
J. M. Gassner, F. Strobl, J. Michelotto, K. Kristina, V. Muth, M. Zimmer, L. Gilles, S. Moosburner, J. Pratschke, I. Sauer, N. Raschzok
Department of Surgery, Experimental Surgery, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany
*Purpose: Small animal models of normothermic ex vivo liver machine perfusion (NEVLP) comparable to clinically used protocols are vital to perform translational studies on organ…
2022 American Transplant Congress
Delivery of Plasmin During Machine Perfusion or Cold Storage Flush Improves Early Transplant Outcomes in aRat Dcd Liver Transplant Model
N. Abraham¹, M. Zhang¹, P. Cray², R. Neill¹, G. Cywinska¹, J. Migaly¹, R. Kahan¹, A. Pontula¹, J. Penaflor¹, Q. Gao¹, K. P. Samy¹, J. J. Pollara², V. Novokhatny³, M. G. Hartwig¹, A. S. Barbas¹
¹Duke Division of Abdominal Transplant Surgery, Duke Ex-Vivo Organ Lab (DEVOL), Durham, NC, ²Duke Division of Surgical Sciences, Pollara lab, Durham, NC, ³Bioscience Industrial Group, Grifols R&D, Durham, NC
*Purpose: Ischemic injury of the peribiliary glands and vascular plexus has been described in DCD livers and correlates with the development of cholangiopathy. Investigators have…
2022 American Transplant Congress
A Randomised Trial Of Normothermic Machine Perfusion Versus Static Cold Storage In Donation After Circulatory Death Renal Transplantation
S. Hosgood¹, C. Callaghan², C. Wilson³, G. Oniscu⁴, B. Phillips⁵, L. Bates³, L. Smith⁶, M. L. Nicholson⁷
¹Surgery, University of Cambridge, Cambridge, United Kingdom, ²Transplant, Guys & St Thomas's, London, United Kingdom, ³Transplant, Freeman Hospital, Newcastle, United Kingdom, ⁴Edinburgh Royal Infirmary, Edinburgh, United Kingdom, ⁵Transplant, Guy's & St Thomas's, London, United Kingdom, ⁶NHSBT, NHSBT, Bristol, United Kingdom, ⁷University of Cambridge, Cambridge, United Kingdom
*Purpose: Kidneys from donation after circulatory death (DCD) donors are more susceptible to cold storage injury and have a high risk of delayed graft function…
2022 American Transplant Congress
Defatting of Steatotic Liver Grafts by Normothermic Ex Vivo Liver Machine Perfusion with 2,4 Dinitrophenol
J. M. Gassner¹, J. Michelotto¹, S. Moosburner¹, F. Strobl¹, A. L. Birkenfeld², A. Kurzbach², G. Shulman³, X. Zhang³, M. Selzner⁴, Y. Noguchi⁴, J. Pratschke¹, I. M. Sauer¹, N. Raschzok¹
¹Department of Surgery, Experimental Surgery, Charité – Universitätsmedizin Berlin, Berlin, Germany, ²Division of Endocrinology, Diabetology and Nephrology, Department of Internal Medicine, Internal, University Hospital Tübingen, Tübingen, Germany, ³Departments of Internal Medicine and Cellular & Molecular Physiology, Yale Diabetes Research Center, Yale School of Medicine, New Haven, CT, ⁴Multi Organ Transplant Program, Ajmera Family Transplant Center, Toronto General Hospital, University of Toronto, Toronto, ON, Canada
*Purpose: The purpose of the study is to demonstrate that steatotic liver grafts can be defatted by normothermic ex vivo liver machine perfusion (NEVLP) with…
2022 American Transplant Congress
Characterisation of Kidney-Specific Extracellular Vesicles During Isolated Organ Normothermic Machine Perfusion
J. Ashcroft¹, T. L. Williams², T. R. Elliott¹, A. Marton¹, S. A. Hosgood¹, M. L. Nicholson¹, V. Kosmoliaptsis¹
¹Department of Surgery, University of Cambridge, Cambridge, United Kingdom, ²Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom
*Purpose: Introduction: Normothermic machine perfusion (NMP) can evaluate and recondition deceased-donor kidneys but accurate biomarkers are needed to assess organ quality and to predict post-transplant…