Articles tagged "Liver preservation"

2017 American Transplant Congress
GSK3β and VDAC in Endoplasmic Reticulum Stress and Apoptosis Modulation During Orthotropic Liver Transplantation.
M. Zaouali,^1,5 A. Panisello Roselló,¹ E. Folch Puy,¹ A. Lopez,² A. Rolo,³ C. Castro,² C. Palmeira,³ A. Garcia-Gil,⁴ R. Adam,² J. Roselló-Catafau.¹
¹Experimental Pathology, Institute of Biomedical Research of Barcelona (IIBB-CSIC), Barcelona, Spain; ²Hepato-Biliare,, Hôpital Paul Brousse, INSERM U-776,Université Paris Sud, Paris, France; ³Center for Neurosciences and Cell Biology, University of Coimbra, Coimbra, Portugal; ⁴Liver Surgery, Hospital Clínico, Zaragoza, Spain; ⁵Research Unit of Biology and Molecular Anthropology Applied to Development and Health (UR12ES11), Faculty of Pharmacy, University of Monastir, Monastir, Tunisia
Ischemia reperfusion injury(IRI) is a major cause of graft non-function following liver transplantation (LT). Mitochondrial dysfunction plays a central role in preventing liver IRI), associated…
2017 American Transplant Congress
Clearance of Transaminases During Normothermic Ex Vivo Liver Perfusion.
M. Bral,¹ N. Aboelnazar,² B. Gala-Lopez,² S. Hatami,² D. Freed,¹ A. Shapiro.¹
¹Department of Transplant Surgery, University of Alberta, Edmonton, AB, Canada; ²Department of Experimental Surgery, University of Alberta, Edmonton, AB, Canada
One of the most promising applications of normothermic ex vivo liver perfusion is the possibility of real-time graft viability assessment. Many liver biomarkers are considered…
2017 American Transplant Congress
The Use of an Haemoglobin Based Oxygen Carrier in a Human Liver Model of Normothermic Machine Perfusion.
Y. Boteon,¹ R. Laing,¹ R. Bogal,¹ D. Neil,² A. Smith,² B. Stephenson,² A. Schlegel,² S. Hübscher,² D. Mirza,² S. Afford,¹ H. Mergental.²
¹Center of Liver Research, University of Birmingham, Birmingham, United Kingdom; ²Liver Unit Queen Elizabeth Hospital, University Hospital Birmingham, Birmingham, United Kingdom
Normothermic machine perfusion of the liver (NMP-L) is a novel technique that preserves liver grafts in an environment that mimics physiological conditions and therefore enables…
2017 American Transplant Congress
Proteasome Modulation an Effective Strategy to Ameliorate Steatotic Liver Grafts Viability After Preservation.
M. Zaouali,¹ A. Panisello Roselló,¹ T. Carbonell,² A. Garcia-Gil,³ A. Lopez,⁴ R. Adam,⁴ J. Rosello-Catafau.¹
¹Patología Experimental, Instituto de Investigaciones Biomédicas de Barcelona, CSIC, Barcelona, Spain; ²Phisiology, Fac. of Biology, Universitat de Barcelona, Barcelona, Spain; ³Transplantation, Hospital Clínico, Zaragoza, Spain; ⁴Hepato- Biliare Unit, Hôpital Paul Brousse, INSERM U-776, Université Paris Sud, Paris, France
Recently we provided evidence for a possible protective role of proteasome inhibition during liver graft preservation. Although, several reported data confirmed that the proteolytic activity…
2017 American Transplant Congress
Prolonged Liver Ischemia Triggers Necrotic Depletion of TIM4+ Kupffer Cells to Facilitate Tissue Inflammatory Immune Activation in Liver Ischemia-Reperfusion Injury.
H. Wang,¹ S. Yue,¹ M. Ni,¹ R. Busittil,¹ J. Kupiec-Weglinski,¹ L. Lu,² Y. Zhai.¹
¹Surgery, David Geffen School of Medicine, UCLA, Los Angeles, CA; ²Liver Surgery, The First affiliated Hospital of Nanjing Medical University, Nanjing, Jiansu Province, China
Although mechanisms of immune activation against liver ischemia-reperfusion injury (IRI) have been studied extensively, questions regarding liver resident Kupffer cells vs. infiltrating macrophages (iM[Oslash]s) remain…
2017 American Transplant Congress
Objective Assessment of Graft Metabolism and Function During Ex Vivo Liver Perfusion.
J. Echeverri,¹ S. Cleland,² M. Kaths,¹ I. Linares,¹ R. Roizales,¹ N. Goldaracena,¹ D. Kollmann,¹ M. Hamar,¹ P. Urbanellis,¹ S. Ganesh,¹ O. Adeyi,³ D. Grant,¹ N. Selzner,⁴ M. Wasowicz,² M. Selzner.¹
¹Multi-Organ Transplant Program, Department of Surgery, University of Toronto - Toronto General Hospital, Toronto, ON, Canada; ²Multi-Organ Transplant Program, Department of Anesthesia, University of Toronto - Toronto General Hospital, Toronto, ON, Canada; ³Multi-Organ Transplant Program, Department of Pathology, University of Toronto - Toronto General Hospital, Toronto, ON, Canada; ⁴Multi-Organ Transplant Program, Department of Medicine, University of Toronto - Toronto General Hospital, Toronto, ON, Canada
During EVLP no objective perfusion parameters have been found to predict outcome after transplantation.Methods: Livers with 4grades of injury were perfused for 4 hours and…
2017 American Transplant Congress
Preliminary Results of Clinical Normothermic Ex Vivo Liver Perfusion in a North American Setting.
M. Bral,¹ B. Gala-Lopez,¹ D. Bigam,¹ N. Kneteman,¹ A. Malcolm,¹ S. Livingstone,¹ A. Andres,¹ J. Emamaullee,¹ L. West,¹ P. Friend,² A. Shapiro.¹
¹Department of Surgery, University of Alberta, Edmonton, AB, Canada; ²Department of Surgery, Nuffield Department of Surgical Sciences, Oxford, United Kingdom; ³Oxford Institute of Biomedical Engineering, Oxford, United Kingdom; ⁴OrganOx, Oxford, United Kingdom
Outcomes of a first clinical normothermic machine perfusion (NMP) UK liver trial demonstrated feasibility and clear safety, with improved liver function when compared to standard…
2017 American Transplant Congress
Normothermic Regional Perfusion of Donors Following Circulatory Death Improves Outcomes in Liver Transplantation.
E. Mowlem,¹ L. Randle,² C. Fear,¹ K. Crick,¹ S. Messer,⁴ S. Large,⁴ A. Butler,³ C. Watson.³
¹Cambridge Transplant Unit, Addenbrookes Hospital, Cambridge, United Kingdom; ²OrganOx Ltd, Oxford, United Kingdom; ³Dept of Surgery, University of Cambridge, Cambridge, United Kingdom; ⁴Papworth Hospital, Cambridge, United Kingdom
Introduction: Donation after Circulatory Death (DCD) provides 23% of UK livers for transplantation, but such transplants are associated with more primary graft function and early…
2017 American Transplant Congress
A Proof of Concept for Liver Graft Modification Using Warm Ex Vivo Perfusion – “It Is Not Just About Rescuing Marginal Grafts”.
N. Goldaracena, J. Echeverri, N. Selzner, D. Grant, J. Feld, M. Selzner.
Toronto General Hospital, University of Toronto, Toronto, Canada
Background: The active metabolism during normothermic ex vivo liver perfusion (NEVLP) offers a new opportunity to use the preservation time for graft modification and repair…
2016 American Transplant Congress
Subnormothermic Machine Perfusion (SNMP) of Liver Allografts with a Novel Hemoglobin-Based Oxygen Carrier (HBOC) Increases β-Oxidation of Fatty Acids and Decreases Lipid Peroxidation (LP).
B. Banan, R. Lopez, C. Hughes, G. Michalopoulos, P. Fontes.
University of Pittsburgh, Pittsburgh.
Introduction: There is limited information about the lipid metabolic profile during liver allograft preservation. LP remains a major component of ischemia-reperfusion injuries (IRIs). The aim…