ATC Abstracts

American Transplant Congress abstracts

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Articles tagged "Liver preservation"

  • 2018 American Transplant Congress

    Gene Silencing with p53 si-RNA Downregulates Inflammatory Markers in the Liver: Potential Utilization during Normothermic Machine Preservation

    C. Moore,1 M. Thijssen,1,2 X. Wang,3 P. Mandrekar,3 E. Xiaofei,3 R. Abdi,4 R. Porte,2 A. Bozorgzadeh,1 H. Leuvenink,2 T. Kowalik,3 P. Martins.1

    1Dept of Surgery, University of Massachusetts, Worcester, MA; 2Dept of Surgery, Groningen University, Gronigen, Netherlands; 3Dept of Medicine, Univ. of Massachusetts, Worcester, MA; 4Transplant Research Institute, Harvard University, Boston, MA.

    Background: RNA interference (RNAi) is a process through which RNA induces the activation of endogenous cellular pathways of RNA degradation, resulting in selective and potent…
  • 2018 American Transplant Congress

    Subnormothermic Machine Perfusion (SNMP) with Human Hemoglobin(Hb)-Based Oxygen Carriers (HBOCs)perfusate Decreased Hepatocellular Reperfusion Injury of Donation after Cardiac Death (DCD) Donor in Pig Model

    T. Shonaka,1 N. Matsuno,1 H. Obara,2 R. Yoshikawa,2 Y. Nishikawa,3 M. Gochi,1 M. Otani,1 H. Takahashi,1 H. Azuma,3 H. Sakai,4 H. Furukawa.1

    1Department of Surgery, Asahikawa Medical University, Asahikawa, Japan; 2Tokyo Metroporitan University, Tokyo, Japan; 3Asahikawa Medical University, Asahikawa, Japan; 4Nara Medical University, Nara, Japan.

    Subnormothermic machine perfusion (SNMP) alleviate hepatoceller injury during organ preservation. However more oxygen is required when perfusate SNMP. Some studies demonstrated that SNMP with hemoglobin-based…
  • 2018 American Transplant Congress

    Human Atrial Natriuretic Peptide (hANP) in Cold Storage of Donation after Cardiac Death Livers: A New Tool for Protecting Vascular Endothelia?

    Y. Nigmet,1 K. Hata,1 Y. Okamura,1 I. Tamaki,1 T. Tsuruyama,2 H. Hirao,1 T. Kubota,1 J. Kusakabe,1 J. Yoshikawa,1 T. Goto,1 T. Tajima,1 H. Tanaka,1 S. Uemoto.1

    1Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery and Transplantation, Kyoto University, Kyoto, Japan; 2Center for Anatomical, Pathological, and Forensic Medical Research, Kyoto University, Kyoto, Japan.

    Aim: Current critical shortage of donor organs has led to acceptance of donation after cardiac death (DCD) livers for transplantation. Human atrial natriuretic peptide (hANP)…
  • 2018 American Transplant Congress

    Supercooling of Human Livers to Extend the Preservation Time for Transplantation

    R. de Vries,1,2 S. Tessier,1,2 P. Banik,1,2 S. Ozer,1,2 S. Napgal,1,2 S. Cronin,1,2 J. Markmann,1,2 H. Yeh,1,2 K. Uygun.1,2

    1Department of Surgery, Massachusetts General Hospital, Boston, MA; 2Center for Engineering in Medicine, Harvard Medical School, Boston, MA.

    Introduction: Extending the maximum preservation time of donor organs could dramatically improve the outcome of organ transplantation. Static cold storage (SCS) at +5[deg]C is the…
  • 2018 American Transplant Congress

    The Role of Ex Vivo Normothermic Preservation in Minimizing Postreperfusion Syndrome in Liver Transplantation

    A. Hassan, J. Cywinski, Q. Liu, D. Pezzati, D. Yang, L. Liu, G. Iuppa, K. Hashimoto, F. Aucejo, M. Fujiki, B. Eghtesad, S. Irefin, J. Fung, K. Abu-Elmagd, C. Miller, C. Quintini.

    Transplant Surgery, Cleveland Clinic, Cleveland.

    Background:Graft reperfusion holds a major challenge during liver transplantation. NMP may improve post-reperfusion hemodynamics, reduce vasopressor and blood product transfusion.Methods:15 patients with liver transplantation after…
  • 2018 American Transplant Congress

    Assessment of Bile Production and Quality during Normothermic Ex Vivo Liver Perfusion to Predict Biliary Injury

    D. Kollmann,1 I. Linares,1 S. Ganesh,1 R. Rosales,1 M. Hamar,1 P. Urbanellis,1 A. Wiebe,1 P. Yip,2 O. Adeyi,2 M. Selzner,1 N. Selzner.1

    1Multi Organ Transplant Program, Toronto General Hospital, Toronto, Canada; 2Laboratory Medicine and Pathobiology, University Health Network, Toronto, Canada.

    Background. Liver transplantation (LT) with donation after circulatory death (DCD)-grafts is associated with higher risk for biliary strictures. We evaluated bile production and quality during…
  • 2017 American Transplant Congress

    GSK3β and VDAC in Endoplasmic Reticulum Stress and Apoptosis Modulation During Orthotropic Liver Transplantation.

    M. Zaouali,1,5 A. Panisello Roselló,1 E. Folch Puy,1 A. Lopez,2 A. Rolo,3 C. Castro,2 C. Palmeira,3 A. Garcia-Gil,4 R. Adam,2 J. Roselló-Catafau.1

    1Experimental Pathology, Institute of Biomedical Research of Barcelona (IIBB-CSIC), Barcelona, Spain; 2Hepato-Biliare,, Hôpital Paul Brousse, INSERM U-776,Université Paris Sud, Paris, France; 3Center for Neurosciences and Cell Biology, University of Coimbra, Coimbra, Portugal; 4Liver Surgery, Hospital Clínico, Zaragoza, Spain; 5Research Unit of Biology and Molecular Anthropology Applied to Development and Health (UR12ES11), Faculty of Pharmacy, University of Monastir, Monastir, Tunisia

    Ischemia reperfusion injury(IRI) is a major cause of graft non-function following liver transplantation (LT). Mitochondrial dysfunction plays a central role in preventing liver IRI), associated…
  • 2017 American Transplant Congress

    Clearance of Transaminases During Normothermic Ex Vivo Liver Perfusion.

    M. Bral,1 N. Aboelnazar,2 B. Gala-Lopez,2 S. Hatami,2 D. Freed,1 A. Shapiro.1

    1Department of Transplant Surgery, University of Alberta, Edmonton, AB, Canada; 2Department of Experimental Surgery, University of Alberta, Edmonton, AB, Canada

    One of the most promising applications of normothermic ex vivo liver perfusion is the possibility of real-time graft viability assessment. Many liver biomarkers are considered…
  • 2017 American Transplant Congress

    The Use of an Haemoglobin Based Oxygen Carrier in a Human Liver Model of Normothermic Machine Perfusion.

    Y. Boteon,1 R. Laing,1 R. Bogal,1 D. Neil,2 A. Smith,2 B. Stephenson,2 A. Schlegel,2 S. Hübscher,2 D. Mirza,2 S. Afford,1 H. Mergental.2

    1Center of Liver Research, University of Birmingham, Birmingham, United Kingdom; 2Liver Unit Queen Elizabeth Hospital, University Hospital Birmingham, Birmingham, United Kingdom

    Normothermic machine perfusion of the liver (NMP-L) is a novel technique that preserves liver grafts in an environment that mimics physiological conditions and therefore enables…
  • 2017 American Transplant Congress

    Proteasome Modulation an Effective Strategy to Ameliorate Steatotic Liver Grafts Viability After Preservation.

    M. Zaouali,1 A. Panisello Roselló,1 T. Carbonell,2 A. Garcia-Gil,3 A. Lopez,4 R. Adam,4 J. Rosello-Catafau.1

    1Patología Experimental, Instituto de Investigaciones Biomédicas de Barcelona, CSIC, Barcelona, Spain; 2Phisiology, Fac. of Biology, Universitat de Barcelona, Barcelona, Spain; 3Transplantation, Hospital Clínico, Zaragoza, Spain; 4Hepato- Biliare Unit, Hôpital Paul Brousse, INSERM U-776, Université Paris Sud, Paris, France

    Recently we provided evidence for a possible protective role of proteasome inhibition during liver graft preservation. Although, several reported data confirmed that the proteolytic activity…
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