Articles tagged "Liver preservation"

2020 American Transplant Congress
Normothermic Machine Perfusion of Rat Liver: Comparing Two Perfusion Methods
F. Zhou, M. Xu, O. Ahmed, G. A. Upadhya, Y. Lin, W. C. Chapman
Surgery, Washington University in St. Louis, St. Louis, MO
*Purpose: Isolated perfused rodent liver (IPRL) model has gained popularity in ex-vivo rat liver preservation and pharmacological intervention studies. Experimental conditions and system layouts vary…
2020 American Transplant Congress
DAMPs and NLRP3 Inflammasome Response in Fatty Liver Grafts Preservation in Different Preservation Solutions
R. G. Bardallo¹, R. G. Teixeira-Silva², A. Panisello-Rosello³, E. Folch-Puy³, J. Rosello Catafau⁴, T. Carbonell⁵
¹Cell Biology, Physiology and Immunology, Faculty of Biology, University of Barcelona, Barcelona, Spain, ²Experimental Pathology, Instituto de Investigaciones Biomedicas de Barcelona, CSIC, Barcelona, Spain, ³Experimental Pathology, Instituto de Investigaciones Biomedicas de Barcelona, Barcelona, Spain, ⁴Experimental Pathology, Instituto de Investigaciones Biomédicas de Barcelona, CSIC, Barcelona, Spain, ⁵Cell Biology, Physiollogy and Immunology, Faculty of Biology, University of Barcelona, Barcelona, Spain
*Purpose: Preservation solutions (UW, HTK and IGL-1) are widely used in clinical liver transplantation. It is well known that the composition of the preservation solution…
2020 American Transplant Congress
TIM-4+ Kupffer Cells Play Homeostatic Roles in Liver Ischemia Reperfusion Injury
M. Ni¹, J. Zhang¹, R. Sosa², S. Kageyama¹, R. Busuttil¹, R. Elaine², J. Kupiec-Weglinski¹, X. Wang³, Y. Zhai¹
¹Dumont-UCLA Transplant Center, Los Angeles, CA, ²Pathology, David Geffen School of Medicine-UCLA, Los Angeles, CA, ³Liver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
*Purpose: Liver ischemia reperfusion injury (IRI) remains a significant clinical problem. Although the molecular mechanism of liver inflammatory response against IR has been studied extensively,…
2020 American Transplant Congress
Hypothermic Machine Perfusion Can Improve Hypoxia Tolerance in Human Discarded Liver
T. Ito, K. Kadono, H. Kojima, S. Kageyama, H. HIrao, K. Nakamura, K. J. Dery, A. E. Murray, J. Aziz, R. W. Busuttil, J. Kupiec-Weglinski, F. M. Kaldas
The Dumont-UCLA Transplant Center, UCLA, Los Angeles, CA
*Purpose: The wider use of livers from expanded criteria donors may help improve access to liver transplantation (LT). Normothermic machine perfusion in LT has been…
2020 American Transplant Congress
The Changes of Transaminases, Lactate Dehydrogenase and pH Levels During Normothermic Liver Preservation Correlate with Early Allograft Dysfunction
C. Bogensperger
Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck, Innsbruck, Austria
*Purpose: Normothermic liver preservation (NMP) has become a clinically established tool to preserve livers in a near-physiological environment. Currently, decreasing lactate and physiological pH-levels are…
2020 American Transplant Congress
Ferroptotic Cell Death During Cold Storage is Essential in Liver Transplant Injury
H. Kojima, S. Kageyama, K. Kadono, H. Hirao, K. J. Dery, T. Ito, K. Nakamura, R. W. Busuttil, J. W. Kupiec-Weglinski
The Dumont-UCLA Transplantation Center, Department of Surgery, Division of Liver and Pancreas Transplantation, David Geffen School of Medicine at UCLA, Los Angeles, CA
*Purpose: It has been reported that activation of apoptosis signaling-regulating kinase 1 (ASK1)/p-p38 axis during tissue cold storage results in ferroptotic cell death. Ferroptosis is…
2020 American Transplant Congress
Comparison of Specificity and Sensitivity of Clinical Viability Criteria to Assess Transplantability of High-Risk Donor Livers
H. Mergental¹, J. Hodson², R. W. Laing¹, Y. L. Boteon¹, J. Attard¹, L. Wallis³, A. Schlegel¹, P. Muiesan¹, M. Abradelo¹, M. P. Perera¹, D. Mirza⁴, S. C. Afford³
¹Liver Unit, Queen Elizabeth Hospital, Birmingham, United Kingdom, ²Department of Statistics, Queen Elizabeth Hospital, Birmingham, United Kingdom, ³Centre for Liver and Gastroenterology Research, University of Birmingham, Birmingham, United Kingdom, ⁴Liver Unit, Darius Mirza, Birmingham, United Kingdom
*Purpose: Normothermic machine perfusion (NMP) of the liver allows objective assessment before transplantation. To date, Birmingham (BC), Cambridge (CC) and Groningen Criteria (GC; Table 1A)…
2020 American Transplant Congress
Therapeutic Targeting of Bile Acid Metabolism Ameliorates Liver Ischemia/Reperfusion Injury
M. Ni¹, D. Jin², T. Lu², J. Zhang¹, R. Busuttil¹, J. Kupiec-Weglinski¹, J. Zhang², Y. Zhai¹
¹Dumont-UCLA Transplant Center, Los Angeles, CA, ²Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China
*Purpose: Farnesoid X receptor (FXR) is the major nuclear receptor of bile acids, which is also involved in the regulation of innate immune responses. As…
2020 American Transplant Congress
CEACAM1 / ASK1 / p38 Signaling Axis Alleviates Peri-Transplant Liver Injury: From Mouse-to-Human
H. Hirao¹, K. Nakamura², S. Kageyama¹, T. Ito¹, K. Kadono¹, H. Kojima¹, K. J. Dery¹, S. Younan¹, R. W. Busuttil¹, F. M. Kaldas¹, J. Kupiec-Weglinski¹
¹UCLA, Los Angeles, CA, ²Kyoto University, Kyoto, Japan
*Purpose: Although CEACAM1 (CM1; carcinoembryonic antigen-related cell adhesion molecule 1; CD66a) regulates hepatic metabolic homeostasis, its role in orthotopic liver transplantation (OLT) remains unknown. A…
2020 American Transplant Congress
Persistent Fibrinolysis Shutdown in Liver Transplant Recipients is Associated with Early Allograph Dysfunction
H. B. Moore¹, T. Nydam¹, J. Pomposelli¹, C. Walker¹, A. Schulick¹, R. Choudhury¹, H. Yaffe¹, M. Adams², M. Cohen³, E. Pomfret¹
¹University of Colorado, Aurora, CO, ²Childrens Hospital, Aurora, CO, ³Denver Health Medical Center, Denver, CO
*Purpose: Early allograph dysfunction (EAD) in liver transplantation has been attributed to microvascular thrombosis. Treating grafts at risk of EAD with tissue plasminogen activator (tPA)…