Articles tagged "knockout"

2018 American Transplant Congress
Glycolytic Bias in mTORC2-Deficient Dendritic Cells Potentiates Antigen-Specific Immunity and Accelerates Graft Rejection
A. Watson,¹ H. Dai,¹ J. Diaz-Perez,² A. Menk,³ M. Killeen,² G. Delgoffe,³ A. Mathers,² A. Thomson.¹
¹Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA; ²Dermatology, University of Pittsburgh, Pittsburgh, PA; ³Cancer Institute, University of Pittsburgh, Pittsburgh, PA.
The mechanistic target of rapamycin (mTOR) is known to function in two complexes: mTOR complex 1 (mTORC1) and mTORC2. While the function of mTORC1 in…
2018 American Transplant Congress
Lymph Node Stromal Laminins Modulate the Dynamic Balance of the Treg/Th17 Axis
L. Li, T. Simon, W. Piao, Y. Xiong, V. Saxena, T. Zhang, C. Wagner, J. Bromberg.
University of Maryland, Baltimore, MD.
Background: Tolerance and immunity are associated with differential expression of lymph node (LN) stromal laminins α4β1γ1 (411) and α5β1γ1 (511), respectively, which determine how alloreactive…
2018 American Transplant Congress
Genetically Modified Porcine Erythrocytes: A Cell Model for Studying Human Preformed Antibody-Dependent Xenoimmune Responses In Vitro
Z. Wang, G. Martens, J. Ladowski, M. Tector, A. Tector.
Surgery, University of Alabama at Birmingham, Birmingham, AL.
Background: Powerful gene editing technology is a promising approach to provide genetically-modified pigs as donors for clinical cell, tissue and organ xenotransplantation. Prior to starting…
2018 American Transplant Congress
Mitochondrial Quality Control through PGC-1α in Syngenic Mouse Heart Transplant
A. Cherry,¹ K. Porter,¹ H. Suliman,¹ J. Wang,² M. Mulvihill,² A. Kirk.²
¹Anesthesiology, Duke University, Durham, NC; ²Surgery, Duke University, Durham, NC.
Intro: Impaired mitochondrial function plays a role in graft dysfunction after transplant, and recovery is facilitated by mitochondrial quality control (MQC). Two key elements of…
2018 American Transplant Congress
Mitochondrial Autophagy Proteins Bnip3 and Nix Maintain Memory B Cell Survival
S. Kodali,¹ H. Sandoval,¹ M. Chen,² J. Wang.¹
¹Immunobiology and Transplant Science Center, Houston Methodist Research Institute, Houston, TX; ²Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX.
Despite the use of immunosuppressive drugs, allograft rejection remains a major hurdle for the long-term success of organ transplants. Burgeoning evidence implicates memory B cells…
2018 American Transplant Congress
T-bet and STAT6 Double Deficiency Favors IL-17 Immunity after Lung Transplantation
F. Liang,¹ R. Chen,¹ Q. Chen,² J. Ochando,³ Y. Ding,¹ J. Xu.¹
¹Department of Immunology, Capital Medical University, Beijing, China; ²Department of Thoracic Surgery, Chaoyang Hospital, Beijing, China; ³Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY.
Background: The long-term success of lung transplantation is mainly limited by the development of obliterative bronchiolitis (OB) in which IL-17 plays a critical role. Direct…
2018 American Transplant Congress
Upregulated IL-33 in Heart Transplants Modulates Local Myeloid Populations to Protect against Chronic Allograft Rejection
Z. Zhang,¹ Q. Liu,¹ G. Hussey,² J. Dziki,² L. Mathews,¹ H. Dai,¹ G. Dwyer,^1,3 A. Lucas,¹ M. Oberbarnscheidt,¹ S. Badylak,² H. Turnquist.^1,3
¹University of Pittsburgh, Starzl Transplant Institute, Pittsburgh, PA; ²University of Pittsburgh, McGowan Institute for Regenerative Medicine, Pittsburgh, PA; ³Immunology, University of Pittsburgh, Pittsburgh, PA.
Purpose: Interleukin-33 (IL-33) is an “alarmin”, or a self-derived molecule that triggers pleotropic immune responses after release from damaged tissue. Yet, how IL-33 shapes alloresponses…
2018 American Transplant Congress
Ablation of Both mTOR and STAT3 in T Cells Induces Heart Transplant Acceptance in Donor Skin-Sensitized Mice
A. Xie,^1,2 W. Chen.^2,3
¹Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China; ²Center for Immunobiology and Transplantation Research, Houston Methodist Research Institute, Houston, TX; ³Department of Surgery, Weill Cornell Medical College of Cornell University, New York, NY.
Allogeneic memory T cell response precludes the induction of transplant acceptance; how this process is regulated at the molecular level remains unclear. Both mTOR and…
2018 American Transplant Congress
Lymph Node Stromal Laminins Affect Graft Survival through Regulation of CD4 T Cell Proliferation and Migration
L. Li, T. Simon, W. Piao, Y. Xiong, V. Saxena, T. Zhang, C. Wagner, J. Bromberg.
University of Maryland, Baltimore, MD.
Background: Our prior studies demonstrated that tolerance and immunity are associated with differential expression of lymph node (LN) stromal laminins α4β1γ1 (411) and α5β1γ1 (511),…
2018 American Transplant Congress
Lymphotoxin Beta Receptor Modulation in Lymphatic Endothelial Cells Alters Regulatory T Cell Dynamics
V. Saxena, W. Piao, Y. Xiong, L. Li, C. Wagner, J. Bromberg.
U Maryland, Baltimore, MD.
Background:Lymphotoxin beta receptor (LTβR) mediated signaling in lymphatic endothelium cells (LEC) is important for migration of regulatory T cells (Treg) to afferent lymphatic vessels (LV)…