Articles tagged "knockout"

2018 American Transplant Congress
Upregulated IL-33 in Heart Transplants Modulates Local Myeloid Populations to Protect against Chronic Allograft Rejection
Z. Zhang,¹ Q. Liu,¹ G. Hussey,² J. Dziki,² L. Mathews,¹ H. Dai,¹ G. Dwyer,^1,3 A. Lucas,¹ M. Oberbarnscheidt,¹ S. Badylak,² H. Turnquist.^1,3
¹University of Pittsburgh, Starzl Transplant Institute, Pittsburgh, PA; ²University of Pittsburgh, McGowan Institute for Regenerative Medicine, Pittsburgh, PA; ³Immunology, University of Pittsburgh, Pittsburgh, PA.
Purpose: Interleukin-33 (IL-33) is an “alarmin”, or a self-derived molecule that triggers pleotropic immune responses after release from damaged tissue. Yet, how IL-33 shapes alloresponses…
2018 American Transplant Congress
Ablation of Both mTOR and STAT3 in T Cells Induces Heart Transplant Acceptance in Donor Skin-Sensitized Mice
A. Xie,^1,2 W. Chen.^2,3
¹Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China; ²Center for Immunobiology and Transplantation Research, Houston Methodist Research Institute, Houston, TX; ³Department of Surgery, Weill Cornell Medical College of Cornell University, New York, NY.
Allogeneic memory T cell response precludes the induction of transplant acceptance; how this process is regulated at the molecular level remains unclear. Both mTOR and…
2018 American Transplant Congress
Lymph Node Stromal Laminins Affect Graft Survival through Regulation of CD4 T Cell Proliferation and Migration
L. Li, T. Simon, W. Piao, Y. Xiong, V. Saxena, T. Zhang, C. Wagner, J. Bromberg.
University of Maryland, Baltimore, MD.
Background: Our prior studies demonstrated that tolerance and immunity are associated with differential expression of lymph node (LN) stromal laminins α4β1γ1 (411) and α5β1γ1 (511),…
2018 American Transplant Congress
Lymphotoxin Beta Receptor Modulation in Lymphatic Endothelial Cells Alters Regulatory T Cell Dynamics
V. Saxena, W. Piao, Y. Xiong, L. Li, C. Wagner, J. Bromberg.
U Maryland, Baltimore, MD.
Background:Lymphotoxin beta receptor (LTβR) mediated signaling in lymphatic endothelium cells (LEC) is important for migration of regulatory T cells (Treg) to afferent lymphatic vessels (LV)…
2018 American Transplant Congress
Role of B Cell Intrinsic NLRP3 in the Development of Chronic Rejection
J. Nie,¹ B. Ramaswami,² X. Guo,³ R. Hoffman,² G. Chalasani.³
¹Thoracic Surgery, Huazhong University of Science and Technology, Wuhan, Hubei, China; ²Surgery, Starzl Transplantation Institute, Pittsburgh, PA; ³Medicine, University of Pittsburgh, Pittsburgh, PA.
Endogenous DAMPs elicited during transplantation are recognized via TLR-MyD88 and NLRP3-inflammosome pathways that are expressed in APCs including B cells. Here, we asked whether B…
2018 American Transplant Congress
Recipient Conditioning Results in IL-33 Support of Potent Th1 Alloimmune Responses after CD4⁺ T Cells Upregulate ST2 during Lymphopenia Induced Proliferation
G. Dwyer,¹ L. Mathews,¹ A. Lucas,¹ B. Blazar,² H. Turnquist.¹
¹Starzl Transplantation Institute and Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA; ²Department of Pediatrics, University of Minnesota, Minneapolis, MN.
Purpose: IL-33 is typically associated with support of Th2 and Treg responses. Yet, recipient conditioning before allogeneic hematopoietic cell transplantation (alloHCT) releases IL-33 to promote…
2018 American Transplant Congress
CCAAT-Enhancer-Binding Protein Homologous Protein Promotes Liver Ischemia and Reperfusion Injury by Inhibiting Beclin-1-Mediated Autophagy in Hepatocytes
H. Zhou,¹ Z. Rao,¹ Y. Xia,¹ R. Liu,¹ S. Zhou,¹ X. Wang,¹ Y. Zhai,¹ L. Lu.¹
¹First Affiliated Hospital of Nanjing Medical University, Nanjing, China; ²David Geffen School of Medicine at University of California-Los Angeles, Los Angeles.
Background and Aims:Critical role of endoplasmic reticulum (ER) stress has been found in ischemia and reperfusion(IR) injury models. However, the role of CCAAT-Enhancer-Binding Protein Homologous…
2018 American Transplant Congress
TGR5-Mediated AMPK/mTOR Signaling Regulates Macrophage Migration and Polarization in Liver Ischemia Reperfusion
S. Zhou, H. Zhou, R. Liu, F. Zhang, X. Wang, L. Lu.
Liver Transplantation Center, First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
Background: Although the plasma membrane-bound G protein–coupled bile acid receptor (TGR5) exerts protective roles in various inflammatory diseases, little is known about the effects and…
2018 American Transplant Congress
Prolongation of Skin Allograft Survival through Dietary Supplementation of Ayrl Hydrocarbon Receptor Ligands
A. Afrazi, L. Owens, J. Fechner, C. O'Driscoll, J. Mezrich.
Transplant Surgery, University of Wisconsin, Madison, WI.
The Aryl Hydrocarbon Receptor (AHR) is a cytosolic transcription factor with numerous endogenous and xenobiotic ligands playing a key role in a number of cellular…
2018 American Transplant Congress
IL23R is a Pathogenic Gene in Type 1 Regulatory T Cells during Mouse Cardiac Transplantation Models
G. Gu, T. Lu, N. Xu, Q. Xia.
Department of Liver Surgery, Renji Hospital, Affiliated by Shanghai Jiaotong University School of Medicine, Shanghai, China.
IL23R has been confirmed to be a pathogenic gene in Th17 cells. However, its role in type 1 regulatory T cells (Tr1 cells) is not…