Articles tagged "knockout"

2018 American Transplant Congress
Role of B Cell Intrinsic NLRP3 in the Development of Chronic Rejection
J. Nie,¹ B. Ramaswami,² X. Guo,³ R. Hoffman,² G. Chalasani.³
¹Thoracic Surgery, Huazhong University of Science and Technology, Wuhan, Hubei, China; ²Surgery, Starzl Transplantation Institute, Pittsburgh, PA; ³Medicine, University of Pittsburgh, Pittsburgh, PA.
Endogenous DAMPs elicited during transplantation are recognized via TLR-MyD88 and NLRP3-inflammosome pathways that are expressed in APCs including B cells. Here, we asked whether B…
2018 American Transplant Congress
Recipient Conditioning Results in IL-33 Support of Potent Th1 Alloimmune Responses after CD4⁺ T Cells Upregulate ST2 during Lymphopenia Induced Proliferation
G. Dwyer,¹ L. Mathews,¹ A. Lucas,¹ B. Blazar,² H. Turnquist.¹
¹Starzl Transplantation Institute and Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA; ²Department of Pediatrics, University of Minnesota, Minneapolis, MN.
Purpose: IL-33 is typically associated with support of Th2 and Treg responses. Yet, recipient conditioning before allogeneic hematopoietic cell transplantation (alloHCT) releases IL-33 to promote…
2018 American Transplant Congress
CCAAT-Enhancer-Binding Protein Homologous Protein Promotes Liver Ischemia and Reperfusion Injury by Inhibiting Beclin-1-Mediated Autophagy in Hepatocytes
H. Zhou,¹ Z. Rao,¹ Y. Xia,¹ R. Liu,¹ S. Zhou,¹ X. Wang,¹ Y. Zhai,¹ L. Lu.¹
¹First Affiliated Hospital of Nanjing Medical University, Nanjing, China; ²David Geffen School of Medicine at University of California-Los Angeles, Los Angeles.
Background and Aims:Critical role of endoplasmic reticulum (ER) stress has been found in ischemia and reperfusion(IR) injury models. However, the role of CCAAT-Enhancer-Binding Protein Homologous…
2018 American Transplant Congress
TGR5-Mediated AMPK/mTOR Signaling Regulates Macrophage Migration and Polarization in Liver Ischemia Reperfusion
S. Zhou, H. Zhou, R. Liu, F. Zhang, X. Wang, L. Lu.
Liver Transplantation Center, First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
Background: Although the plasma membrane-bound G protein–coupled bile acid receptor (TGR5) exerts protective roles in various inflammatory diseases, little is known about the effects and…
2018 American Transplant Congress
Prolongation of Skin Allograft Survival through Dietary Supplementation of Ayrl Hydrocarbon Receptor Ligands
A. Afrazi, L. Owens, J. Fechner, C. O'Driscoll, J. Mezrich.
Transplant Surgery, University of Wisconsin, Madison, WI.
The Aryl Hydrocarbon Receptor (AHR) is a cytosolic transcription factor with numerous endogenous and xenobiotic ligands playing a key role in a number of cellular…
2018 American Transplant Congress
IL23R is a Pathogenic Gene in Type 1 Regulatory T Cells during Mouse Cardiac Transplantation Models
G. Gu, T. Lu, N. Xu, Q. Xia.
Department of Liver Surgery, Renji Hospital, Affiliated by Shanghai Jiaotong University School of Medicine, Shanghai, China.
IL23R has been confirmed to be a pathogenic gene in Th17 cells. However, its role in type 1 regulatory T cells (Tr1 cells) is not…
2018 American Transplant Congress
NOS2 Deficiency Limits Long Term Survival of Murine Kidney Allografts
I. Aljabban,^1,2 B. Jiang,¹ C. Yang,¹ T. O'Shea,¹ D. Ndishabandi,¹ R. White,¹ P. Russell,¹ R. Colvin,¹ J. Madsen,¹ A. Alessandrini.¹
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA; ²Pennsylvania State University College of Medicine, Hershey, PA.
Nitric oxide (NO) is a small molecule derived from L-arginine that governs a myriad of physiologic processes including the antimicrobial and tumoricidal responses. Although NO…
2018 American Transplant Congress
Loss of HIF-1α in CD4 T Cell Improve Outcome of Mouse Vascularized Osteomyocutaneous Allotransplantation under Costimulation Blockade
C-.H. Lin,^1,2 M. Anggelia,^1,2 H. Yang,³ W. Chuang,⁴ H. Cheng,¹ F-.C. Wei,^1,2 G. Brandacher.⁵
¹PRS, Center for VCA, Chang Gung Memorial Hospital, Taoyuan, Taiwan; ²Chang Gung Medical College and Chang Gung University, Taoyuan, Taiwan; ³Nephrology, Chang Gung Memorial Hospital, Taoyuan, Taiwan; ⁴Pathology, Chang Gung Memorial Hospital, Taoyuan, Taiwan; ⁵PRS, Johns Hopkins University School of Medicine, Baltimore, MD.
Background: Allograft survival depends on the balance of effector T cells (Teff) and regulatory T cells (Tregs). Previous study suggested that the balance is controlled…
2018 American Transplant Congress
Glycolytic Bias in mTORC2-Deficient Dendritic Cells Potentiates Antigen-Specific Immunity and Accelerates Graft Rejection
A. Watson,¹ H. Dai,¹ J. Diaz-Perez,² A. Menk,³ M. Killeen,² G. Delgoffe,³ A. Mathers,² A. Thomson.¹
¹Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA; ²Dermatology, University of Pittsburgh, Pittsburgh, PA; ³Cancer Institute, University of Pittsburgh, Pittsburgh, PA.
The mechanistic target of rapamycin (mTOR) is known to function in two complexes: mTOR complex 1 (mTORC1) and mTORC2. While the function of mTORC1 in…
2018 American Transplant Congress
Lymph Node Stromal Laminins Modulate the Dynamic Balance of the Treg/Th17 Axis
L. Li, T. Simon, W. Piao, Y. Xiong, V. Saxena, T. Zhang, C. Wagner, J. Bromberg.
University of Maryland, Baltimore, MD.
Background: Tolerance and immunity are associated with differential expression of lymph node (LN) stromal laminins α4β1γ1 (411) and α5β1γ1 (511), respectively, which determine how alloreactive…