Articles tagged "Ischemia"

2017 American Transplant Congress
Direct Comparison of Hypothermic and Normothermic Organ Preservation in a Porcine Ex-Vivo Kidney Model.
N. Vallant,^1,2 B. Sandhu,¹ N. Wolfhagen,¹ C. Pusey,¹ V. Papalois.¹
¹Renal and Transplant Department, Imperial College, London, United Kingdom; ²Department of Visceral-, Transplant- and Thoracic Surgery, Medical University, Innsbruck, Austria
Background: Hypothermic machine perfusion is a well established method for deceased donor organ preservation as well as pre-transplant assessment and preconditioning. Recent translational and clinical…
2017 American Transplant Congress
Ischemia-Reperfusion Injury-Induced Innate Immune Signaling in Human Orthotopic Liver Transplantation.
R. Sosa,¹ A. Zarrinpar,² M. Rossetti,¹ C. Lassman,¹ B. Naini,¹ N. Datta,² R. Busuttil,² D. Gjertson,¹ J. Kupiec-Weglinski,^1,2 E. Reed.¹
¹Pathology and Laboratory Medicine, University of California, Los Angeles, CA; ²Surgery, University of California, Los Angeles, CA
Background: Ischemia-reperfusion injury (IRI) is a major risk factor in the development of acute and chronic graft rejection in orthotopic liver transplantation (OLT); however, no…
2017 American Transplant Congress
Down-Regulation of Nuclear HMGB1 by Small Interfering RNA Protects Against Liver Ischemia-Reperfusion Injury.
G. Zhao, C. Fu, L. Wang, L. Zhu, S. Chen, G. Chen.
Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, HUST, Wuhan, Hubei, China
Background:High mobility group box 1 (HMGB1) was found to have both a beneficial intracellular role and injurious extracellular role after a sterile inflammatory insult.Hepatocyte-specific HMGB1…
2017 American Transplant Congress
Gene Editing of Keap1/Nrf2 in Human T Cells Using CRISPR-Cas9 to Improve Outcome from Ischemia Reperfusion Injury and Other T Cell Mediated Diseases.
S. Noel,¹ S. Lee,¹ M. Sadasivam,² A. Hamad,² H. Rabb.¹
¹Department of Medicine, Johns Hopkins University, Baltimore, MD; ²Department of Pathology, Johns Hopkins University, Baltimore, MD
Background: T lymphocytes are important mediators of ischemia reperfusion injury (IRI) during organ transplantation. Using genetically engineered mice, we demonstrated that augmentation of Nrf2 dependent…
2017 American Transplant Congress
Low Level Light Laser Treatment Reduces Macrophage Retention and ROS Levels Following Tissue Injury.
A. Paredes,¹ D. Benavidez,¹ R. Patil,¹ B. Khalil,¹ M. Mitchell,¹ J. Cheng,¹ S. Mangos,² A. Bartholomew.¹
¹University of Illinois, Chicago; ²Rush University, Chicago
Following ischemia reperfusion injury, inflamed macrophages switch from oxidative phosphorylation to glycolysis; in contrast, macrophages associated with tissue repair rely on oxidative phosphorylation. The triggers…
2017 American Transplant Congress
Vendor to Center in 0-60: A Safe and Effective Blueprint for Creating an In-House On-Call System for Organ Offers.
K. Driggers, J. Davenport, D. Cassidy, L. Curry, A. Milling, A. Hauser, H. Crego, P. Baliga, N. Pilch, D. Dubay, J. Anderson.
Transplant, Medical University of South Carolina, Charleston, SC
Introduction: Our center returned to an in-house nurse coordinator on-call program 7/2016 after using a vendor from 11/2012 to 7/2016 for organ allocation. It was…
2017 American Transplant Congress
Succinate Accumulation During Warm and Cold Ischemia in Mouse, Pig and Man: Mechanistic and Therapeutic Implications for Transplant Ischemia-Reperfusion Injury.
J. Martin,^1,3 A. Costa,² A. Gruszczyk,^1,3 M. Hamed,¹ N. Georgakopoulos,¹ G. Pettigrew,¹ A. James,³ C. Frezza,² M. Murphy,³ K. Saeb-Parsy.¹
¹Department of Surgery, Cambridge, United Kingdom; ²MRC Cancer Unit, Cambridge, United Kingdom; ³MRC Mitochondrial Biology Unit, Cambridge, United Kingdom
Introduction: The burst of reactive oxygen species associated with ischemia-reperfusion (IR) injury is mediated through a specific metabolic pathway involving mitochondrial accumulation of the metabolite…
2017 American Transplant Congress
Novel Application of Localized Nanodelivery of Anti-IL6 Protects Organ Transplant from Ischemia Reperfusion Injuries.
M. Uehara,¹ Z. Solhjou,¹ B. Bahmani,¹ O. Maarouf,¹ T. Ichimura,² C. Brooks,² W. Xu,¹ V. Kasinath,¹ N. Banouni,¹ A. Elkhal,³ I. Guleria,² M. McGrath,¹ S. Tullius,³ R. Abdi.¹
¹Transplantation Research Center/Renal Devision, Brigham and Women's Hospital/ Harvard Medical School, Boston; ²Renal Division, Brigham and Women's Hospital/ Harvard Medical School, Boston; ³Division of Transplant Surgery and Transplantation Surgery Research Laboratory, Brigham and Women's Hospital/ Harvard Medical School, Boston
Ischemia reperfusion injury (IRI) evokes major intra-graft inflammatory responses that markedly augment alloimmune responses. Understanding mechanisms involved is critical for the development of novel therapeutic…
2017 American Transplant Congress
Objective Assessment of Graft Metabolism and Function During Ex Vivo Liver Perfusion.
J. Echeverri,¹ S. Cleland,² M. Kaths,¹ I. Linares,¹ R. Roizales,¹ N. Goldaracena,¹ D. Kollmann,¹ M. Hamar,¹ P. Urbanellis,¹ S. Ganesh,¹ O. Adeyi,³ D. Grant,¹ N. Selzner,⁴ M. Wasowicz,² M. Selzner.¹
¹Multi-Organ Transplant Program, Department of Surgery, University of Toronto - Toronto General Hospital, Toronto, ON, Canada; ²Multi-Organ Transplant Program, Department of Anesthesia, University of Toronto - Toronto General Hospital, Toronto, ON, Canada; ³Multi-Organ Transplant Program, Department of Pathology, University of Toronto - Toronto General Hospital, Toronto, ON, Canada; ⁴Multi-Organ Transplant Program, Department of Medicine, University of Toronto - Toronto General Hospital, Toronto, ON, Canada
During EVLP no objective perfusion parameters have been found to predict outcome after transplantation.Methods: Livers with 4grades of injury were perfused for 4 hours and…
2017 American Transplant Congress
CD47 Blockade Reduces Ischemia/Reperfusion Injury in Donation After Cardiac Death Rat Kidney Transplantation.
X. Wang,¹ M. Xu,¹ J. Jia,¹ Z. Zhang,¹ J. Gaut,² G. Upadhya,¹ P. Manning,³ Y. Lin,¹ W. Chapman.¹
¹Department of Surgery, Washington University School of Medicine, Saint Louis; ²Pathology and Immunology, Washington University School of Medicine, Saint Louis; ³Tioma Therapeutics, Saint Louis
Purpose: We evaluated the potential effects of antibody-mediated CD47 blockade to modulate vascular endothelial nitric oxide activity and reduce ischemia reperfusion injury in a rat…