Articles tagged "Ischemia"

2022 American Transplant Congress
Delivery of Plasmin During Machine Perfusion or Cold Storage Flush Improves Early Transplant Outcomes in aRat Dcd Liver Transplant Model
N. Abraham¹, M. Zhang¹, P. Cray², R. Neill¹, G. Cywinska¹, J. Migaly¹, R. Kahan¹, A. Pontula¹, J. Penaflor¹, Q. Gao¹, K. P. Samy¹, J. J. Pollara², V. Novokhatny³, M. G. Hartwig¹, A. S. Barbas¹
¹Duke Division of Abdominal Transplant Surgery, Duke Ex-Vivo Organ Lab (DEVOL), Durham, NC, ²Duke Division of Surgical Sciences, Pollara lab, Durham, NC, ³Bioscience Industrial Group, Grifols R&D, Durham, NC
*Purpose: Ischemic injury of the peribiliary glands and vascular plexus has been described in DCD livers and correlates with the development of cholangiopathy. Investigators have…
2022 American Transplant Congress
Investigating RNA Interference-Mediated Inhibition of Liver Ischemia Reperfusion Injury During Liver Preservation
J. E. Buchwald¹, D. Echeverria², J. Sousa², N. McHugh², A. Khvorova², P. N. Martins³
¹RNA Therapeutics Institute, Department of Surgery, University of Massachusetts Chan Medical School, Worcester, MA, ²RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA, ³Department of Surgery, University of Massachusetts Chan Medical School, Worcester, MA
*Purpose: Liver transplantation cures acute end-stage and chronic liver diseases, yet current strategies of organ preservation including static cold storage (SCS) and machine perfusion (MP)…
2022 American Transplant Congress
Type 1 Innate Lymphoid Cells Alleviate Acute Liver Injury Following Transplantation via Interferon-γ Mediated Pathway
H. Kojima, K. Kadono, H. Hirao, K. J. Dery, J. Kupiec-Weglinski, Y. Zhai
Dumont-UCLA Transplant Center, Los Angeles, CA
*Purpose: Innate lymphoid cells (ILCs), a heterogeneous population of non-B non-T lymphocytes, have been found in recent years to plays key regulatory roles in tissue…
2022 American Transplant Congress
Identification of Akkermansia Muciniphila and Bacteroides Thetaiotaomicron Associated with the Alleviation of Peritransplant Liver Damage in Mouse Recipients
K. Dery¹, H. Kojima², H. Hirao², K. Kadono³, T. Dong², J. Kupiec-Weglinski⁴
¹Surgery, UCLA, Los Angeles, CA, ²UCLA, Los Angeles, CA, ³Liver Transplant Surgery, UCLA, Los Angeles, CA, ⁴UCLA Medical Center, Los Angeles, CA
*Purpose: Although recent evidence shows that modifications of gut microbiota by antibiotics (Abx) influences liver allograft function, its compositional role in orthotopic liver transplantation (OLT)…
2022 American Transplant Congress
HDAC6 Inhibition Mediated Protection from Hepatic Ischemia Reperfusion is Associated with Preserved Glutathione Levels
C. OBrien¹, P. Hernandez¹, S. Concors², Z. Wang¹, G. Ge³, A. Kozikowski⁴, W. Hancock⁵, M. Levine²
¹Surgery, University of Pennsylvania, Philadelphia, PA, ²University of Pennsylvania, Philadelphia, PA, ³Surgery, University of Pensylvania, Philadelphia, PA, ⁴College of Pharmacy, University of Illinois at Chicago, Chicago, IL, ⁵Childrens Hospital of Philadelphia, Philadelphia, PA
*Purpose: Liver Ischemia reperfusion injury (IRI) and Acetaminophen (APAP) toxicity are important causes of acute liver failure in the United States, that lack effective therapy.…
2022 American Transplant Congress
Role of Mitochondrial Transplantation in Enhancing Liver Regeneration Following Partial Hepatectomy
G. Lamanilao¹, C. Watkins¹, C. Kuscu¹, C. Kuscu¹, J. Eason², A. Bajwa¹
¹Transplant Research Institute, James D. Eason Transplant Institute, Department of Surgery, The University of Tennessee Health Science Center, Memphis, TN, ²University of Tennessee/Methodist Transplant Institute, Memphis, TN
*Purpose: Therapeutic strategies that accelerate the rate of liver regeneration following partial hepatectomy (PH) should be developed to reduce occurrences of small for size syndrome…
2022 American Transplant Congress
Normothermic Machine Perfusion of the Liver Supports Protein Translation and Mitochondrial Function While Reducing Protein Degradation and Metabolic Imbalance: A Proteomics Study
L. Lo Faro¹, A. Thorne², H. Huang³, P. Charles³, M. Kaisar¹, S. Davis³, F. Dengu¹, S. Shaheed¹, J. Mulvey¹, R. Fischer³, D. Nasralla⁴, B. Kessler³, H. Leuvenink², P. Friend¹, R. Ploeg¹
¹Nuffield Department of Surgical Sciences, University of Oxford, Oxford, United Kingdom, ²University Medical Center Groningen, Groningen, Netherlands, ³Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom, ⁴Royal Free London NHS Foundation Trust, London, United Kingdom
*Purpose: Liver preservation by normothermic machine perfusion (NMP) involves perfusion of the graft with oxygenated blood and nutrients. The NMP Liver trial by the Consortium…
2022 American Transplant Congress
Impact of Cold Ischemia Times on 1 Year Kidney Transplant Outcomes
T. Fletchinger, L. Burdine
Department of Surgery, University of Arkansas for Medical Sciences, Little Rock, AR
*Purpose: Recent organ allocation changes have increased travel and subsequent cold ischemia times (CIT) of kidney allografts. We sought to investigate the impact of prolonged…
2022 American Transplant Congress
An Immune-Modulatory Strategy to Mitigate Hepatic Ischemia Reperfusion Injury in a Murine Model
S. Duarte, A. Kobayashi, V. Boominathan, A. Kwiatkowski, J. Simonovich, A. Wanchoo, G. Hudalla, B. Keselowsky, A. Zarrinpar
University of Florida, Gainesville, FL
*Purpose: Hepatic ischemia/reperfusion injury (IRI) is the leading cause of early graft dysfunction and contributes to the shortage of donor liver grafts. However, despite its…
2022 American Transplant Congress
Normothermic Ex Vivo Pancreas Perfusion for the Preservation of Porcine Pancreas Grafts
C. Parmentier, S. Ray, L. Mazilescu, M. Kawamura, T. Goto, Y. Noguchi, B. Arulratnam, S. Ganesh, M. Selzner, T. Reichman
Multi-Organ Transplant Department, University Health Network - Toronto General Hospital, Toronto, ON, Canada
*Purpose: The purpose of this study was to develop a stable porcine model for normothermic ex-vivo pancreas perfusion (NEVPP) for the preservation of grafts with…