Articles tagged "Ischemia"

2020 American Transplant Congress
Ex Vivo Normothermic Perfusion Improves Cold Ischemic Injury and DGF in an Experimental Model of DBD Kidney Transplantation
A. Gutierrez-Dalmau¹, V. Alastrue², Á. García De Jalon³, M. Sánchez³, C. Del Agua⁴, V. P. González⁵, Á. Roncales³, J. Gómez-Arrue⁶, C. Pastor⁶, B. Sáez⁷, L. M. Esteban⁸, M. J. Gil³, M. Doblaré⁹, Á. Borque-Fernando³
¹Nephrology, Hospital Universitario Miguel Servet (HUMS), IIS Aragón, Zaragoza, Spain, ²EBERS, Zaragoza, Spain, ³Urology, HUMS, Zaragoza, Spain, ⁴Pathology, HUMS, Zaragoza, Spain, ⁵Hematology, HUMS, Zaragoza, Spain, ⁶Experimental Surgery, IACS, Zaragoza, Spain, ⁷Faculty of Health Sciences, San Jorge University, Zaragoza, Spain, ⁸EUPLA, Zaragoza University, Zaragoza, Spain, ⁹Department of Mechanical Engineering, Zaragoza University, Zaragoza, Spain
*Purpose: Cold ischemic (CI) injury results in high rates of DGF, reduced graft function and survival. Ex vivo normothermic perfusion (EVNP) has shown to improve…
2020 American Transplant Congress
Successful Transplantation of Murine Hearts Preserved 24h Under Subzero Ischemia Using Novel Bio-Inspired Next Generation Cryoprotectants
B. Oh¹, A. Callegari², M. Kline², A. Childs², M. Iglesias Lozano¹, G. Brandacher¹, X. Wei²
¹Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA), Johns Hopkins University School of Medicine, Baltimore, MD, ²X-Therma Inc., Richmond, CA
*Purpose: Extension of the organ ischemia window is of paramount importance to prolong viability of transplantable organs and to improve overall outcomes. Transport using a…
2020 American Transplant Congress
A Novel Polymer Product, APP-103, Reduces IRI-Induced Damage and Promotes Functional Improvement in Syngeneic Models of Renal Transplantation
K. Minami¹, S. Bae², H. Uehara¹, J. Iske¹, J. Reder³, H. Azuma⁴, A. Weins¹, E. Van Keuren⁵, B. Houser³, A. El-Khal¹, P. Kang², S. Tullius¹
¹Brigham and Women's Hospital, Boston, MA, ²Beth Israel Deaconess Medical Center, Boston, MA, ³Celdara Medical, Lebanon, NH, ⁴Osaka Medical College, Osaka, Japan, ⁵Georgetown University, Washington DC, DC
*Purpose: Our data introduce APP-103 as a novel, non-toxic and site-activating therapeutic approach that effectively ameliorates the consequences of ischemia/reperfusion injury in solid organ transplantation.*Methods:…
2020 American Transplant Congress
Dual and Opposite Costimulatory Targeting with a Novel Hybrid Protein Effectively Prevents Renal Warm Ischemia Reperfusion Injury in a Rat Bilateral Kidney Model
J. Guiteras¹, L. De Ramon¹, E. Crespo¹, N. Bolaños¹, P. Fontova¹, M. Jarque¹, A. Torija¹, O. Bestard², J. Grinyó², J. Torras²
¹Experimental Nephrology, IDIBELL, Hospitalet de Llobregat, Spain, ²Bellvitge University Hospital, Hospitalet de Llobregat, Spain
*Purpose: Many studies showed the CTLA4 effectiveness in the inhibition of CD28-CD80/86 costimulatory pathway in transplant and autoimmune diseases. Furthermore, the PD1-PDL1/2 interaction has been…
2020 American Transplant Congress
Hif-1a Expression Influences Recipient Clinical Outcomes in Liver Transplant Patients
K. Kadono, T. Ito, H. Kojima, K. Nakamura, H. Hirao, S. Kageyama, K. J. Dery, A. Murray, R. W. Busutill, F. Kaldas, J. W. Kupiec-Weglinski
Liver Transplant Surgery, UCLA, Los Angeles, CA
*Purpose: Liver ischemia-reperfusion injury (IRI), an innate-immune dominated hepatocellular damage, is a risk factor for early allograft dysfunction (EAD) and rejection crises in liver transplantation…
2020 American Transplant Congress
Rat Hind Limb Transplant Model to Assess Vascularized Composite Allograft (VCA) Inflammation and Ischemia Reperfusion Injury
Z. Wang¹, A. B. Harrant¹, Y. Wang¹, K. Washington¹, E. Farkash², C. A. Huang¹
¹Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO, ²Pathology, University of Michigan, Ann Arbor, MI
*Purpose: Vascularized composite allografts are potential surgical solutions for patients with traumatic or disfiguring injuries but are highly immunogenic and require elevated levels of immunosuppression.…
2020 American Transplant Congress
Normothermic Ex Vivo Perfusion of Marginal Liver Grafts Rapidly Stimulates Release of Graft-Derived Exosomes and Markedly Decreases the Inflammatory Pathways Induced at Early Times after Transplant Reperfusion
K. E. Brown¹, K. S. Keslar¹, D. Margeta¹, Q. Liu², A. Simioni², L. Del Angel Diaz², W. M. Baldwin¹, R. L. Fairchild¹, C. Quintini²
¹Lerner Research Institute, Cleveland Clinic, Cleveland, OH, ²Transplant Center, Cleveland Clinic, Cleveland, OH
*Purpose: Normothermic ex vivo machine perfusion of liver grafts (EVLP) is emerging as a superior preservation technology to cold storage. Normothermic EVLP transforms a metabolically…
2020 American Transplant Congress
Older Organs Transfer Senescence to Transplant Recipients
J. Iske¹, R. Maenosono¹, M. Seyda¹, T. Heinbockel¹, M. Koichiro¹, Y. Nian¹, T. Tchkonia², J. L. Kirkland², R. Abdi¹, A. Elkhal¹, S. G. Tullius¹
¹Brigham and Women's Hospital, Boston, MA, ²Mayo Clinic, Rochester, MN
*Purpose: Older organs represent an underutilized potential. Organ age, at the same time, augments alloimmune responses resulting into increased acute rejection rates. Senescent cells accumulate…
2020 American Transplant Congress
Humanized Mouse Reveals the Cytoprotective Function of Hepatocyte RXFP1 in Liver Transplant Recipients
H. Kojima¹, S. Kageyama¹, K. Kadono¹, H. Hirao¹, K. J. Dery¹, T. Ito¹, G. Sugahara², Y. Ishida², T. Saito², F. M. Kaldas¹, R. W. Busuttil¹, J. W. Kupiec-Weglinski¹
¹Surgery, The Dumont-UCLA Transplantation Center, Los Angeles, CA, ²Division of Gastrointestinal and Liver Disease, Keck School of Medicine, USC, Los Angeles, CA
*Purpose: RXFP1 (RLX family peptide receptor 1) is a cognate receptor for relaxin (RLX), an insulin-like growth factor, with vasoactive/remodeling properties. We have documented cytoprotective…
2020 American Transplant Congress
Human Organ Culture: A Novel Method for Evaluation of Reperfusion Injury in Human Kidney
J. T. Langford¹, C. Edwards¹, J. DiRito², M. Harris¹, S. A. Hosgood³, M. L. Nicholson³, J. S. Pober⁴, D. Haakinson¹, G. T. Tietjen¹
¹Department of Surgery, Yale School of Medicine, New Haven, CT, ²Department of Biomedical Engineering, Yale University, New Haven, CT, ³Department of Surgery, University of Cambridge, Cambridge, United Kingdom, ⁴Department of Immunobiology, Yale School of Medicine, New Haven, CT
*Purpose: Evaluate the ability of human organ culture to assess protein secretion during a model of reperfusion injury.*Methods: Biopsies are obtained from transplanted-declined human kidneys…