Articles tagged "Ischemia"

2020 American Transplant Congress
Older Organs Transfer Senescence to Transplant Recipients
J. Iske¹, R. Maenosono¹, M. Seyda¹, T. Heinbockel¹, M. Koichiro¹, Y. Nian¹, T. Tchkonia², J. L. Kirkland², R. Abdi¹, A. Elkhal¹, S. G. Tullius¹
¹Brigham and Women's Hospital, Boston, MA, ²Mayo Clinic, Rochester, MN
*Purpose: Older organs represent an underutilized potential. Organ age, at the same time, augments alloimmune responses resulting into increased acute rejection rates. Senescent cells accumulate…
2020 American Transplant Congress
Humanized Mouse Reveals the Cytoprotective Function of Hepatocyte RXFP1 in Liver Transplant Recipients
H. Kojima¹, S. Kageyama¹, K. Kadono¹, H. Hirao¹, K. J. Dery¹, T. Ito¹, G. Sugahara², Y. Ishida², T. Saito², F. M. Kaldas¹, R. W. Busuttil¹, J. W. Kupiec-Weglinski¹
¹Surgery, The Dumont-UCLA Transplantation Center, Los Angeles, CA, ²Division of Gastrointestinal and Liver Disease, Keck School of Medicine, USC, Los Angeles, CA
*Purpose: RXFP1 (RLX family peptide receptor 1) is a cognate receptor for relaxin (RLX), an insulin-like growth factor, with vasoactive/remodeling properties. We have documented cytoprotective…
2020 American Transplant Congress
Human Organ Culture: A Novel Method for Evaluation of Reperfusion Injury in Human Kidney
J. T. Langford¹, C. Edwards¹, J. DiRito², M. Harris¹, S. A. Hosgood³, M. L. Nicholson³, J. S. Pober⁴, D. Haakinson¹, G. T. Tietjen¹
¹Department of Surgery, Yale School of Medicine, New Haven, CT, ²Department of Biomedical Engineering, Yale University, New Haven, CT, ³Department of Surgery, University of Cambridge, Cambridge, United Kingdom, ⁴Department of Immunobiology, Yale School of Medicine, New Haven, CT
*Purpose: Evaluate the ability of human organ culture to assess protein secretion during a model of reperfusion injury.*Methods: Biopsies are obtained from transplanted-declined human kidneys…
2020 American Transplant Congress
Contamination of Acinar Cells Impairs Therapeutic Effect of Islet Transplantation Due to Induction of Inflammatory Reaction in Not Only Graft Islets but Also Hepatic Parenchyma
K. Chinen, G. Yoshimatsu, N. Sakata, S. Kodama
Regenerative Medicine and Transplantation, Fukuoka University, Fukuoka City, Japan
*Purpose: Islet transplantation is a promising treatment option for type 1 diabetes. Edmonton protocol recommended that over 30 % purity of islets is essential for…
2020 American Transplant Congress
Microbiota Modulates Ischemia-Reperfusion Injury in Mouse Liver Transplantation
S. Kageyama¹, H. Kojima¹, K. Kadono¹, H. Hirao¹, K. J. Dery¹, K. Nakamura², T. Dong³, J. Jacobs³, R. W. Busuttil¹, J. W. Kupiec-Weglinski¹
¹Surgery, Dumont-UCLA Transplant Center, Los Angeles, CA, ²Surgery, Nishi Kobe Medical Center, Kobe, Japan, ³The Vatche and Tamar Manoukian Division of Digestive Diseases, David Geffen School of Medicine, Los Angeles, CA
*Purpose: Ischemia-reperfusion injury (IRI) is a major risk factor in orthotopic liver transplantation (OLT). As host microbiota may associate with health or disease, antibiotics (Abx)…
2020 American Transplant Congress
p40 Homodimers Indirectly Induce Endogenous Donor-Reactive Memory CD8 T Cell Activation within High-Risk Cardiac Allografts and CTLA-4ig Resistant Graft Rejection via IL-15 Signaling
H. Tsuda, A. Valujskikh, R. Fairchild
Inflammation and Immunity, Cleveland Clinic, Cleveland, OH
*Purpose: We have reported that longer duration of cold ischemic storage (CIS) prior to transplant resulted in increased proliferation of endogenous donor-reactive CD8 T cells…
2020 American Transplant Congress
Tissue Specificity and the Role of Lymphocytes in Estrogen-Mediated Protection from Renal Ischemia Reperfusion Injury
P. T. Hernandez, C. S. O'Brien, S. J. Concors, L. N. Krumeich, Z. Wang, G. Ge, W. Hancock, M. H. Levine
Hospital of the University of Pennsylvania, Philadelphia, PA
*Purpose: Renal ischemia reperfusion injury (IRI) is a major contributor to delayed graft function (DGF) in kidney transplantation. We previously demonstrated lower rates of DGF…
2020 American Transplant Congress
Impact of Liver Graft Preservation Methods on the Gut Microbiome and Metabolome
G. A. Cresci¹, N. Sangwan², A. Simioni³, Q. Liu³, C. Quintini³
¹Pediatric Gastroenterology and Inflammation & Immunity, Cleveland Clinic, Cleveland, OH, ²Lerner Research Institute, Cleveland Clinic, Cleveland, OH, ³Digestive Disease and Surgery Institute, Cleveland Clinic, Cleveland, OH
*Purpose: Normothermic ex-vivo liver perfusion (NEVLP) is a powerful platform for organ preservation and assessment. It induces metabolic reactivation that potentially helps clear accumulated toxic…
2020 American Transplant Congress
Effect of Cold Ischemia Time on Long-Term Graft Survival of Post-One Year Survivor Deceased Donor Kidney Transplant Recipients
B. Murthy¹, H. Nguyen², A. Rana¹, A. Awan¹, C. Van Buren³, J. Goss¹
¹Baylor College of Medicine, Houston, TX, ²University of Texas Medical Branch, Galveston, TX, ³University of Texas Houston Medical School, Houston, TX
*Purpose: Prolonged cold ischemia time (CIT) is known to adversely affect short-term graft survival among renal allografts. However, its effect on long-term graft survival is…
2020 American Transplant Congress
The Pro-Inflammatory Profile of Alcoholic Patients Increases Risk for Ischemia-Reperfusion Injury During Orthotopic Liver Transplantation
A. Q. Terry¹, R. A. Sosa¹, M. Rossetti¹, F. Li¹, J. Nevarez-Mejia¹, B. V. Naini¹, F. M. Kaldas², V. M. Groysberg¹, S. M. Younan², R. W. Busuttil², D. W. Gjertson¹, J. W. Kupiec-Weglinski³, E. F. Reed¹
¹Department of Pathology and Laboratory Medicine, UCLA, Los Angeles, CA, ²Department of Surgery, UCLA, Los Angeles, CA, ³Departments of Pathology and Laboratory Medicine and Surgery, UCLA, Los Angeles, CA
*Purpose: Ischemia-reperfusion injury (IRI) elicits cellular damage which triggers a pro-inflammatory immune response that can lower allograft survival in orthotopic liver transplant (OLT) patients. In…