Articles tagged "Genomics"

2016 American Transplant Congress
Molecular Assessment of Kidney Transplant Biopsies Performs Similarly in Medulla and Cortex.
K. Madill-Thomsen,¹ J. Reeve,¹ G. Bohmig,² F. Eskandary,² P. Halloran.^1,3
¹Alberta Transplant Applied Genomics Center, Edmonton, AB, Canada; ²Department of Nephrology and Dialysis, Medical University of Vienna, Vienna, Austria; ³Department of Medicine, Division of Nephrology and Transplant Immunology, University of Alberta, Edmonton, AB, Canada.
Histologic assessment of kidney biopsies is usually unable to interpret medulla, but molecular changes associated with rejection likely occur in both medulla and cortex. Analysis…
2016 American Transplant Congress
VDJ Immune Repertoire Sequencing Predicts Patients at Risk of Alloimmune Injury.
M. Sirota,¹ T. Sigdel,¹ S. Boyd,² A. Fire,² M. Sarwal.¹
¹Surgery and Bioinformatics, UCSF, San Francisco, CA; ²Genetics, Stanford University, Palo Alto, CA.
Background: The complex repertoire of immune receptors generated by B cells enables allo-recognition in the context of transplantation across HLA barriers, and is a major…
2016 American Transplant Congress
Analysis of T Cell Receptor Repertoire in Routine Renal Allograft Biopsy.
R. Reindl-Schwaighofer,¹ J. Weinberger,¹ R. Jimenez-Heredia,¹ J. Sunzenauer,¹ S. Schaller,² S. Winkler,² R. Oberbauer.¹
¹Department of Nephrology, Medical University of Vienna, Vienna, Austria; ²Bioinformatics Research Group, University of Applied Sciences Upper Austria, Hagenberg, Austria.
Introduction: Diversity and clonality of the T cell repertoire of circulating and tissue-resident T-cells can be assessed by sequencing of recombined T cell receptor (TCR)…
2016 American Transplant Congress
Exome Sequencing and HLA-Independent Prediction of Long-Term Kidney Allograft Function.
L. Mesnard,^1,3,6 T. Muthukumar,^2,9 M. Burbach,² C. Li,² H. Shang,⁴ D. Dadhania,^2,3 J. Lee,^2,3 V. Sharma,² J. Xiang,⁴ M. Carmagnat,⁵ C. Suberbielle,⁵ N. Ouali,⁶ E. Rondeau,^6,1 J. Friedewald,⁸ M. Abecassis,⁷ M. Suthanthiran,^2,9 F. Campagne.³
¹UMR1155, INSERM, Paris, France; ²Division of Nephrology and Hypertension, New York Presbyterian Hospital, New York; ³Department of Physiology and Biophysics, The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, New York, NY; ⁴Genomics Core Facility, Weill Cornell Medical College, New York, NY; ⁵Laboratoire d'histocompatibilité Hôpital Saint Louis, APHP, Paris, France; ⁶Service des Urgences Néphrologiques et Transplantation Rénale, APHP, Paris, France; ⁷Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine, Chicago, IL; ⁸Northwestern University Feinberg School of Medicine, Chicago, IL; ⁹Department of Transplantation Medicine, Weill Cornell Medical College, New York, NY.
Current strategies to improve graft outcome following kidney transplantation consider information at the HLA loci. We asked whether information outside of the HLA loci can…
2016 American Transplant Congress
Impact of Ethnicity on Tacrolimus Trough/Dose Ratios Following Solid Organ Transplantation.
A. Santeusanio, A. Owusu-Obeng, J. Jeff.
Mount Sinai Hospital, New York.
Objective: to assess tacrolimus dosing requirements based upon ethnicity in order to help guide future decisions regarding dosage initiation and titration among Hispanic and African…
2016 American Transplant Congress
A Genome Wide Association Study of New Onset Diabetes After Transplant in Kidney Transplantation.
A. Giri,¹ M. Sanders,² D. Velez Edwards,¹ T. Ikizler,³ D. Roden,³ K. Birdwell.³
¹Epidemiology, Vanderbilt University Medical Center, Nashville, TN; ²Medicine, University of Iowa, Iowa City, IA; ³Medicine, Vanderbilt University Medical Center, Nashville, TN.
Purpose: New-onset diabetes after transplantation (NODAT) is associated increased mortality. Using a DNA biobank linked to electronic medical records (EMR) at Vanderbilt University (BioVU), we…
2016 American Transplant Congress
Differences in Tacrolimus Troughs and Doses Between Caucasians and African Americans with Two CYP3A5 Loss-of-Function Alleles: Looking Beyond CYP3A5 Expression.
W. Oetting,¹ D. Schladt,² W. Guan,¹ R. Remmel,¹ C. Dorr,² K. Sanghavi,¹ A. Matas,¹ R. Mannon,³ A. Israni,² P. Jacobson,¹ For DeKAF Genomics,¹ GEN-03 Investigators.¹
¹University of Minnesota, Minneapolis, MN; ²Hennepin County Medical Center and Minneapolis Medical Research Foundation, Minneapolis, MN; ³University of Alabama, Birmingham, AL.
Higher tacrolimus (TAC) dose requirements and lower TAC troughs (TT) in African Americans (AA) relative to Caucasians (CA) have been presumed to be due to…
2015 American Transplant Congress
Single Nucleotide Polymorphisms (SNPs) Associated With Acute Rejection in European American Kidney Transplant Recipients: A Genome Wide Association Study (GWAS)
A. Israni,¹ C. Dorr,¹ M. Miller,² D. Schladt,¹ K. Sanghavi,³ A. Muthusamy,¹ R. Remmel,³ W. Guan,⁴ B. Wu,⁴ W. Oetting,³ P. Jacobson,³ A. Matas,⁵ For DeKAF Genomics & GEN03 Investigators.¹
¹Medicine, MMRF, Hennepin County Medical Center, Minneapolis, MN; ²Psychology, University of MN, Minneapolis, MN; ³Pharmacy, University of MN, Minneapolis, MN; ⁴Biostatistics, University of MN, Minneapolis, MN; ⁵Surgery, University of MN, Minneapolis, MN.
Purpose: Acute rejection (AR) is associated with increased risk of allograft loss. Therefore, we conducted a GWAS to determine SNPs associated with AR in a…
2015 American Transplant Congress
Differentially Expressed Genes in Immunosuppressed Kidney Transplant Recipients
C. Dorr,¹ B. Wu,² W. Guan,² A. Muthusamy,¹ K. Sanghavi,³ P. Jacobson,³ W. Oetting,³ D. Schladt,¹ R. Remmel,³ J. Maltzman,⁵ A. Matas,⁴ For DeKAF Genomics Investigators,¹ A. Israni.¹
¹Medicine, MMRF, HCMC, Minneapolis, MN; ²Biostatistics, University of MN, Minneapolis, MN; ³Pharmacy, University of MN, Minneapolis, MN; ⁴Surgery, University of MN, Minneapolis, MN; ⁵Medicine, University of PA, Philadelphia, PA.
Introduction: In our effort to personalize immunosuppressive therapies we performed RNA sequencing (RNAseq) to identify differentially expressed genes (DEGs) after kidney transplant (tx) and the…
2015 American Transplant Congress
Influence of POR*28 Genotype on Tacrolimus Disposition in Heart Transplant Recipients
T. Alford,¹ R. Page,¹ J. Lindenfeld,² C. Aquilante.¹
¹University of Colorado Skaggs School of Pharmacy and Pharmaceutical Sciences, Aurora, CO; ²University of Colorado School of Medicine, Aurora, CO.
Polymorphisms in P450 oxidoreductase (POR) have been shown to modulate CYP3A activity and alter the pharmacokinetics of CYP3A substrates, such as tacrolimus (TAC). The objective…