Articles tagged "Genomic markers"

2022 American Transplant Congress
Kidney Allograft Injury is Reflected in the DNA Cargo of Urinary Extracellular Vesicles
I. Sedej¹, M. Štalekar², M. Tušek Žnidarič², K. Goričar³, N. Kojc⁴, P. Kogovšek², V. Dolžan³, M. Lenassi³, M. Arnol¹
¹Department of Nephrology, University Medical Centre Ljubljana, Ljubljana, Slovenia, ²Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia, ³Institute of Biochemistry and Molecular Genetics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia, ⁴Institute of Pathology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
*Purpose: Following cell injury, donor-derived cell-free DNA (ddcfDNA) is increased in the urine of kidney transplant recipients. DNA containing urinary extracellular vesicles (uEVs) are relevant…
2022 American Transplant Congress
Infection Impacts dd-cfDNA in Lung Transplant Recipients
D. Levine¹, C. Giner¹, C. Pham², R. Woodward², A. Roux³
¹University of Texas Health System San Antonio, San Antonio, TX, ²CareDX, South San Francisco, CA, ³Pneumology, Adult CF Center and Lung Transplantation Department, Foch Hospital, Suresnes, France
*Purpose: Infection is the leading cause of death within 1 year post lung transplant. Graft injury secondary to infection is affected by both source and…
2022 American Transplant Congress
Trends in Donor Derived Cell Free DNA Before and After Cellular and Antibody Mediated Rejection
K. Guo¹, J. Friedewald¹, C. Rebello¹, B. Kinsella¹, S. Park², L. Zhao¹
¹Northwestern University, Chicago, IL, ²Transplant nephrology, Northwestern University, Chicago, IL
*Purpose: The TRAC dd-cfDNA test was developed to detect acute kidney transplant rejection but can also detect subclinical rejection with improved performance in antibody-mediated rejection…
2022 American Transplant Congress
An Initial Analysis of Baseline Levels of Dd-cfdna After Pancreas Transplantation: A Prospective Study from High-Volume Centers in the United States
A. Yoo¹, I. Qian¹, A. Riedel¹, G. Kibria¹, A. Bartosic¹, R. Soltani², O. Nduanya³, M. Omidi³, J. S. Odorico², P. L. Abrams³, M. Cooper³, J. S. Bromberg¹, J. R. Scalea¹
¹University of Maryland School of Medicine, Baltimore, MD, ²University of Wisconsin School of Medicine, Madison, WI, ³Georgetown University School of Medicine, Washington, DC
*Purpose: Donor-derived cell-free DNA (dd-cfDNA) testing, which has been validated for monitoring kidney transplant (KTx) rejection, may provide an alternative to pancreas biopsy that prevents…
2022 American Transplant Congress
Kinetics of dd-cfDNA in Kidney Transplant Recipients Following Sars-CoV-2 Vaccination Booster Administration
N. M. Ali¹, S. Mehta¹, V. Tatapudi¹, A. Mattoo¹, I. Soomro¹, J. Benstein¹, H. Neumann¹, F. Zervou¹, J. Miles², R. Montgomery¹
¹Transplant Institute, NYU Langone Health, New York, NY, ²CareDx, Brisbane, CA
*Purpose: Evolving data suggests booster vaccine doses enhance the immunogenicity of SARS-CoV-2 vaccines in solid organ transplant recipients with higher IgG responses, neutralizing antibodies titers,…
2021 American Transplant Congress
Validation of a Novel Gene Expression Biomarker of Rejection Following Liver Transplantation
J. Levitsky¹, M. Kandpal¹, T. Whisenant², K. Guo¹, S. Kurian³, M. Abecassis⁴
¹Gastroenterology & Hepatology; Comprehensive Transplant Center, Northwestern University, Chicago, IL, ²University of California San Diego, San Diego, CA, ³Scripps Clinic, La Jolla, CA, ⁴University of Arizona College of Medicine Tucson, Tucson, AZ
*Purpose: Non-invasive biomarkers distinguishing early signs of immune activation vs. quiescence could more objectively guide immunosuppression management after liver transplantation (LT). Our aim was to…
2021 American Transplant Congress
The Role of Donor-derived Cell-free DNA to Detect Subclinical Acute Rejection in Kidney Allograft
S. Park¹, K. Guo², R. Heilman³, E. Poggio⁴, D. Taber⁵, C. Marsh⁶, S. Kurian⁷, S. Kleiboeker⁸, J. Weems⁹, J. Holman¹⁰, L. Zhao², R. Sinha⁸, S. Brietigam², C. Rebello², M. Abecassis¹¹, J. Friedewald¹
¹Transplant nephrology, Northwestern University, Chicago, IL, ²Feinberg School of Medicine, Northwestern University, Chicago, IL, ³Transplant nephrology, Mayo Clinic College of Medicine and Science, Phoenix, AZ, ⁴Department of Nephrology and Hypertension, Cleveland Clinic, Cleveland, OH, ⁵Division of Transplant Surgery, Medical University of South Carolina, Charleston, SC, ⁶Department of Medicine and Surgery, Scripps Clinic and Green Hospital, La Jolla, CA, ⁷Scripps Health, La Jolla, CA, ⁸Eurofins US Clinical Diagnostics, Lee's Summit, MO, ⁹Eurofins US Clinical Diagnostics, Lee’s Summit, MO, ¹⁰Transplant Genomics, Inc, Mansfield, MA, ¹¹University of Arizona College of Medicine, Northwestern University, Tucson, AZ
*Purpose: We hypothesized that donor-derived cell-free DNA (dd-cfDNA) could detect subclinical acute rejection (subAR).*Methods: 429 blood samples paired with surveillance kidney biopsies from 208 patients…
2021 American Transplant Congress
Dd-cfdna Can Guide Safe Reintroduction of Immunosuppression in Kidney Transplant Recipients with Covid-19
J. Miles¹, J. Leonard², V. Tatapudi², M. Fei¹, R. Montgomery², N. M. Ali²
¹CareDx, Brisbane, CA, ²Transplant Institute, NYU Langone Health, New York, NY
*Purpose: COVID-19 infection is associated with 25% mortality in kidney transplant recipients (KTRs). Reduction of anti-metabolite immunosuppressants during the acute COVID-19 illness is a common…
2021 American Transplant Congress
Cellular Landscape of Steatotic Livers Revealed by Single-nuclei Rna Sequencing
C. Kuscu¹, C. Kuscu¹, A. Shetty², E. Bardhi³, T. Rousselle⁴, J. Eason¹, M. Sraj⁵, D. Maluf⁶, V. Mas⁴
¹Surgery, James D Eason Transplant Institute, Memphis, TN, ²University of Maryland, Institute for Genome Sciences, Baltimore, MD, ³Program in Transplantation - University of Maryland, Baltimore, MD, ⁴Surgery, Division of Surgical Science, Baltimore, MD, ⁵Surgery, University of Maryland, Baltimore, MD, ⁶Surgery, Program in Transplantation - University of Maryland, Baltimore, MD
*Purpose: Hepatic IRI represents a barrier to the use of available organs for transplant, particularly fatty livers. We aimed to discern the cellular subsets and…
2021 American Transplant Congress
Association Between Donor-Recipient Genetic Matching and Acute Rejection in Kidney Transplantation
R. Cao¹, V. Arthur², J. Chen², B. Keating², C. Dorr³, D. Schladt³, G. Onyeaghala³, R. Mannon⁴, A. Matas¹, R. Remmel¹, N. Pankratz¹, B. Wu¹, W. Oetting¹, P. Jacobson¹, A. Israni³, W. Guan¹
¹University of Minnesota, Minneapolis, MN, ²University of Pennsylvania, Philadelphia, PA, ³Hennepin Healthcare Research Institute, Minneapolis, MN, ⁴University of Nebraska, Omaha, NE
*Purpose: In this study we carried out a genome-wide association study (GWAS) between donor-recipient matching scores at individual SNP level and Acute rejection (AR) in…