Articles tagged "Gene expression"

2017 American Transplant Congress
Mechanistic Insights by RNA Profiling on Rejecting Vascularized Composite Allotransplants.
H.-Y. Cheng,¹ S.-C. Liu,² C.-F. Lin,¹ L.-Y. Shih,³ F.-C. Wei.^1,2,3
¹Center for Vascularized Composite Allotransplantation, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; ²College of Medicine, Chang Gung University, Taoyuan, Taiwan; ³Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan
Background: Detailed molecular changes associated with rejection to vascularized composite allotransplants (VCA) are not well understood. Materials and Methods: peripheral blood was drawn from the…
2017 American Transplant Congress
Gene Expression During Development of Chronic Antibody-Mediated Rejection in Renal Allografts from Non-Human Primates: Validation of Markers Used in Humans and Sequential Changes in Protocol Biopsies.
B. Adam,¹ R. Smith,² M. Matsunami,² I. Rosales,² B. Afzali,¹ T. Oura,² A. Cosimi,² T. Kawai,² R. Colvin,² M. Mengel.¹
¹University of Alberta, Edmonton, Canada; ²Harvard Medical School and Massachusetts General Hospital, Boston
Background: RNA transcript measurement is a promising adjunct for the diagnosis and classification of antibody-mediated rejection (ABMR), however most technologies require specially processed biopsy samples.…
2017 American Transplant Congress
Molecular Classifier of Antibody-Mediated Rejection Is a Better Predictor of Histologic Rejection Than a Corresponding Transcript Set and Is Further Improved When Combined with Classifiers of Rejection Syndromes.
K. Famulski, P. Halloran.
University of Alberta, Edmonton, AB, Canada
Molecular and histologic changes in graft rejection are not completely specific, often being induced by injuries and other diseases. We examined various methods for using…
2017 American Transplant Congress
Age-Related Differences in Gene Expression Among Kidney Allografts with Normal Histology and Calcineurin Inhibitor Nephrotoxicity.
E. Rhone,¹ S. Bontha,¹ P. Walker,² C. Dumur,³ D. Maluf,¹ J. Almenara,³ V. Mas.¹
¹University of Virginia, Charlottesville; ²Arkana Laboratories, Little Rock; ³Virginia Commonwealth University, Richmond
Background Most biomarker research first occurs in adult populations, with consequent extrapolation to children. However, age-related differences may impact gene expression, such as variations in…
2017 American Transplant Congress
Similar Elevation of Gene Expression Associated with Antibody-Mediated Rejection (ABMR) of Renal Allografts Can Be Detected in Both Plasma Exosomes (pExo) and Biopsies (Bx) by Assessing mRNA Transcript Profiles.
H. Zhang,¹ C. Nast,² J. Doldron,² M. Cheung,² J. Mirocha,³ J. Kahwaji,¹ A. Vo,¹ S. Jordan,¹ M. Toyoda.¹
¹Transplant Immunology Laboratory, Cedars-Sinai Medical Center, Los Angeles; ²Pathology, Cedars-Sinai Medical Center, Los Angeles; ³Biostatistics Center, Cedars-Sinai Medical Center, Los Angeles
Background: ABMR is a major obstacle for successful transplant in HLA-sensitized patients (Pts). Prediction of ABMR by non-invasive diagnostic tools is desirable. Previously we showed…
2017 American Transplant Congress
Clinical and Molecular Significance of Microvascular Inflammation Negative Transplant Glomerulopathy.
M. Lubetzky,¹ N. Hayde,¹ P. O Broin,² Y. Bao,¹ E. Akalin.¹
¹Montefiore Medical Center, Bronx; ²National University of Ireland Galway University, Galaway, Ireland
Transplant glomerulopathy (TGP) with donor specific antibodies (DSA) and microvascular inflammation (MVI) is due to chronic antibody mediated rejection (CAMR). We reported previously that intragraft…
2017 American Transplant Congress
Specific Gene Expression Signature of Complement-Activating Donor Specific Anti-HLA Antibody-Mediated Rejection in Kidney Allografts.
C. Lefaucheur,¹ D. Viglietti,¹ L. Hidalgo,² O. Aubert,¹ A. Zeevi,³ P. Halloran,² A. Loupy.¹
¹Paris Translational Research Center for Organ Transplantation, Paris, France; ²University of Alberta, Edmonton, Canada; ³University of Pittsburgh Medical Center, Pittsburgh
Complement-binding anti-HLA DSA have demonstrated higher rejection rate and decreased allograft survival. However, their specific effects on rejection pathogenesis have not been identified. We investigated…
2017 American Transplant Congress
Molecular Significance of Peritubular Capillaritis in Early Transplant Kidney Biopsies of Donor-Specific Antibody Negative Patients.
M. Ajaimy, P. O Broin, Y. Bao, E. Akalin.
Montefiore Medical Center Transplant Center, Albert Einstein College of Medicine, Bronx
Background: Peritubular capillaritis (PTC) is a part of microvascular inflammation seen in antibody-mediated rejection. However, it can be observed as an isolated lesion in early…
2016 American Transplant Congress
Single Cell Profiling of Th9 Cells Allows Identification of Functionally Different Subsets.
K. Stanko, J. Schumann, K. Vogt, S. Schlickeiser, C. Appelt, B. Sawitzki.
Institute of Medical Immunology, Charite University Medicine, Berlin, Germany.
T helper type 9 (Th9) cells represent a distinct subset of CD4+ effector T cells that is characterized by high secretion of interleukin (IL)-9. Protective…
2016 American Transplant Congress
Assessment of mRNA Gene Profiles in Plasma Exosome to Predict Risk for Antibody-Mediated Rejection (ABMR) of Renal Allografts.
H. Zhang,¹ J. Kahwaji,¹ P. Li,² S. Ge,¹ D. Thomas,¹ C. Nast,³ A. Vo,¹ M. Haas,³ S. Jordan,¹ M. Toyoda.¹
¹Transplant Immunology Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA; ²Surgery, Cedars-Sinai Medical Center, Los Angeles, CA; ³Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA.
Background: ABMR is a major obstacle for successful transplant in HLA-sensitized (HS) patients. Complement-mediated and antibody-dependent cellular cytotoxicity (ADCC) are primarily mechanisms for ABMR. Previously…