Articles tagged "Gene expression"

2018 American Transplant Congress
mRNA Transcript Profiling of 3 Genes in Renal Allograft Biopsies (Bx) Improves Accuracy of Antibody-Mediated Rejection (ABMR) Diagnosis in Transplant Recipients Desensitized with IVIG+Rituximab+Immunoglobulin G-Degrading Enzyme of Streptococcus Pyogenes (IdeS)
H. Zhang,¹ C. Nast,² J. Doldron,² M. Cheung,² A. Vo,³ S. Jordan,³ M. Toyoda.¹
¹Transplant Immunology Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA; ²Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA; ³Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA.
Background: Multiple molecular signatures specific to ABMR based on gene expression in renal allograft Bx of transplant recipients were reported. Here we assessed mRNA transcript…
2018 American Transplant Congress
Predictors of Graft Survival at the Time of a Kidney Transplant Indication Biopsy
G. Einecke,¹ J. Reeve,^2,3 P. Halloran.^2,4
¹Hannover Medical School, Hannover, Germany; ²Alberta Transplant Applied Genomics Centre, Edmonton, Canada; ³Laboratory Medicine and Pathology, University of Alberta, Edmonton, Canada; ⁴Medicine, University of Alberta, Edmonton, Canada.
We predicted kidney transplant graft survival after indication biopsy in 884 patients. Analysis was performed using random forests, a multivariate machine learning method to assess…
2018 American Transplant Congress
Overexpression of BAX, NOL3, and XIAP Apoptotic Genes Participates in Calcineurin Inhibitors Nephrotoxicity: New Insights into Chronic Allograft Dysfunction
L. Garcia-Covarrubias,¹ G. Queipo,² M. Fonseca,² D. Alcantara,¹ P. Prieto,¹ H. Diliz,¹ A. Garcia,¹ H. Hinojosa,¹ A. Cicero,¹ G. Melendez,³ M. Alamilla,³ R. Lascurain,⁴ V. Soto.⁵
¹Transplantation, Hospital General de Mexico, Mexico City, Mexico; ²Genetics, Hospital General de Mexico, Mexico City, Mexico; ³Research, Hospital General de Mexico, Mexico City, Mexico; ⁴Faculty of Medicine, UNAM, Mexico City, Mexico; ⁵Pathology, Hospital General de Mexico, Mexico City, Mexico.
Introduction: Calcineurin inhibitors toxicity (CNIT) is a factor that limit the outcome of renal transplantation graft. The hypoxia causes histological data as nodular hyalinosis, hyalinosis,…
2018 American Transplant Congress
Hypothermic Machine Perfusion Protects DCD Liver Graft through Metabolism of Xenobiotics by Cytochrome P450 Pathway
Q. Ye,^1,2 W. Liang,¹ Z. Zhong,¹ S. Ye,¹ B. Tang,¹ Q. Hu,¹ J. Liang,¹ Y. Wang.¹
¹Transplant Center of Wuhan University, Zhongnan Hospital of Wuhan University, Wuhan, China; ²The 3rd Xiangya Hospital of Central South Unversity, Changsha, China.
Background: Donation after cardiac death (DCD) is the mainly resource to relieve organ-transplantation shortage. Our previous research have confirmed that the function of DCD liver…
2018 American Transplant Congress
Molecular Assessment of Pre-Implantation Donor Biopsies: Effects of Prolonged Cold Ischemia Time and Development of Delayed Graft Function
E. Akalin,¹ Y. Bao,¹ V. Bontha,² M. e Silva,³ M. Ajaimy,¹ P. [Oacute] Broin,³ V. Mas.²
¹Montefiore Medical Center, Bronx; ²University of Virginia, Charlottesville; ³National University of Ireland Galway, Galway, Ireland.
Background: Deceased-donor (DD) kidneys are at higher risk for ischemia/reperfusion injury leading to increased inflammatory mediators and innate immune response. We aimed to investigate the…
2018 American Transplant Congress
Validation Study of Gene Expression Signatures Associated with Antibody-Mediated Rejection (ABMR) and Cell-Mediated Rejection (CMR) in Renal Allograft Biopsies (Bx) Suggests Complexity of Molecular Diagnosis for ABMR Compared to CMR
H. Zhang,¹ C. Nast,² J. Doldron,² M. Cheung,² A. Vo,³ S. Jordan,³ M. Toyoda.¹
¹Transplant Immunology Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA; ²Pathology, Cedars-Sinai Medical Center, Los Angeles, CA; ³Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA.
Background: Various molecular signatures specific to ABMR and CMR based on gene expression in renal allograft Bx or peripheral blood mononuclear cells (PBMC) of renal…
2018 American Transplant Congress
Hypothermic Machine Perfusion Protects Rabbit Kidney Graft through Down Regulation SPP1-MMP7 Signaling Axis
Q. Hu,¹ Z. Zhong,¹ S. Xue,¹ W. Liang,¹ J. Lan,¹ J. Liang,¹ Y. Xiong,¹ Y. Wang,¹ Q. Ye.^1,2
¹Zhongnan Hospital of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan, China; ²The 3rd Xiangya Hospital of Central Sourth University, Changsha, China.
Background: Multicenter studies have indicated that hypothermic machine perfusion(HMP) can be used to prolong cold ischemia time with high-quality preservation and allow utilization of kidney…
2018 American Transplant Congress
Identification of Noninvasive and Mechanistically-Informative Biomarkers Predictive of Reversal of Acute Cellular Rejection (ACR) in Kidney Allografts
D. Luan,¹ J. Schwartz,² R. Ding,² T. Muthukumar,² D. Dadhania,² M. Suthanthiran.²
¹University of Michigan, Ann Arbor, MI; ²Weill Cornell Medical College, New York City, NY.
Objective: We determined whether urinary cell mRNAs predict response to anti-rejection therapy in kidney allograft recipients with Banff grade IA or higher ACR biopsies.Methods: We…
2018 American Transplant Congress
Renal Allograft Molecular Pathways Associated with Tolerance Induction in Renal Transplantation
L. Gallon,¹ J. Mathew,¹ S. Bontha,² C. Dumur,² P. Dalal,¹ L. Nadimpalli,¹ D. Maluf,² A. Shetty,¹ S. Ildstad,³ J. Leventhal,¹ V. Mas.²
¹Department of Medicine-Nephrology, Northwestern University, Chicago, IL; ²Department of Surgery, University of Virginia, Charlottesville, VA; ³Departments of Surgery, Physiology and Immunology, University of Louisville, Louisville, KY.
Background: Chronic exposure to immunosuppressive agents for organ transplant results in graft nephrotoxicity, infections and malignancies We have established durable immune tolerance in mismatched related…
2018 American Transplant Congress
Intragraft Gene Expression Differentiates Polyomavirus Nephropathy from T-Cell Mediated Rejection
B. Adam,¹ S. Wagner,¹ J. Bräsen,² V. Bröcker,² V. D'Agati,³ C. Drachenberg,⁴ E. Farkash,⁵ A. Farris,⁶ L. Geldenhuys,⁷ A. Magil,⁸ V. Nickeleit,⁹ P. Randhawa,¹⁰ H. Regele,¹¹ M. Mengel.¹
¹University of Alberta, Edmonton, Canada; ²Hannover Medical School, Hannover, Germany; ³Columbia University, New York; ⁴University of Maryland, College Park; ⁵University of Michigan, Ann Arbor; ⁶Emory University, Atlanta; ⁷Dalhousie University, Halifax, Canada; ⁸University of British Columbia, Vancouver, Canada; ⁹University of North Carolina, Chapel Hill; ¹⁰University of Pittsburgh, Pittsburgh; ¹¹Medical University of Vienna, Vienna, Austria.
Background: Improved immunosuppression protocols have reduced the incidence of T-cell mediated rejection (TCMR) but still carry a significant risk of BK polyomavirus nephropathy (BKVN). Despite…