Articles tagged "Gene expression"

2019 American Transplant Congress
Single Cell Analysis of Kidney Allograft Rejection
C. M. Castro-Rojas¹, K. M. Roskin², C. G. Moore², B. Abu Jawdeh³, N. Salomonis², R. R. Alloway⁴, E. Woodle⁴, D. A. Hildeman¹
¹Immunobiology, Cincinnati Children's Hospital, Cincinnati, OH, ²Biomedical Informatics, Cincinnati Children's Hospital, Cincinnati, OH, ³Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, ⁴Transplantation, University of Cincinnati College of Medicine, Cincinnati, OH
*Purpose: Rejection therapies (corticosteroids and antilymphocyte globulins) have not changed in over 50 years. In part this is due to a knowledge deficit of signaling…
2019 American Transplant Congress
Molecular Phenotype of Proliferative Arteriopathy in Kidney Grafts
A. Perkowska-Ptasinska¹, M. Ciszek¹, D. Deborska-Materkowska¹, A. Debska-Slizien², P. F. Halloran³, M. Myslak⁴
¹Warsaw Medical University, Warsaw, Poland, ²Gdansk Medical University, Gdansk, Poland, ³University of Alberta, Edmonton AB, AB, Canada, ⁴Pomeranian Medical University, Szczecin, Poland
*Purpose: Proliferative arteriopathy (PA) is a lesion characterized by a fibrotic tissue in the arterial intima. The phenomenon is marked by the lack of elastic…
2019 American Transplant Congress
Correlation between Gene Expression and Histology in Transbronchial Lung Transplant Biopsies
B. Adam, K. Du, S. Rotich, M. Mengel
University of Alberta, Edmonton, AB, Canada
*Purpose: Transbronchial lung transplant biopsies (TBBx) are limited by non-specific histologic lesions and suboptimal interobserver reproducibility. Gene expression offers a potentially more precise, objective and…
2019 American Transplant Congress
The Role of CD47/SIRPα and CD47/TSP-1 Pathways in Pig-to-Baboon Kidney Transplantation Using hCD47 Transgenic Kidneys
K. Takeuchi¹, Y. Ariyoshi¹, T. Pomposelli¹, L. Boyd¹, D. Ekanayake-Alper¹, S. Arn¹, R. Hawley¹, D. Ayares², M. Lorber³, M. Sykes¹, D. Sachs¹, R. Johnson⁴, K. Yamada¹
¹CCTI/Surgery, Columbia University Medical Center, New York, NY, ²Revivicor Inc., Blacksburg, VA, ³Lung BioTechnology PBC, Silver Spring, MD, ⁴Division of Renal Diseases and Hypertension, University of Colorado, Aurora, CO
*Purpose: We have recently reported that both CD80 upregulation and the degradation of SMPDL-3b on glomerular podocytes are involved in the development of post kidney…
2019 American Transplant Congress
Integrative Analysis of Immune Repertoire and Gene Expression from RNA-seq in Kidney Organ Transplants
S. Pineda¹, A. Ravoor², O. Bestard³, M. Sarwal¹, M. Sirota²
¹Department of Surgery, UCSF, San Francisco, CA, ²BCHSI, UCSF, San Francisco, CA, ³Department of Surgery, 3Bellvitge University Hospital, Barcelona, Spain
*Purpose: The main problem with kidney transplantation is the possibility of rejection, which is classified as either antibody-mediated rejection (AMR) or T-cell-mediated rejection (TCMR) depending…
2019 American Transplant Congress
Automated Histology Lesion Interpretation in Kidney Transplant Biopsies Shows That Pathologists Often Deviate from Banff Guidelines
K. S. Madill-Thomsen¹, J. Reeve², G. Bohmig³, F. A. Eskandary⁴, A. Perkowska-Ptasinska⁵, M. Myslak⁶, P. F. Halloran and the INTERCOMEX Study Group¹
¹University of Alberta, Edmonton AB, AB, Canada, ²Alberta Transplant Applied Genomics Centre, Edmonton, AB, Canada, ³Medical University of Vienna, Vienna, Austria, ⁴Medical University of Vienna, Edmonton AB, AB, Canada, ⁵Medical University of Warsaw, Warsaw, Poland, ⁶Pomeranian Medical University, Szczecin, Poland
*Purpose: Histologic diagnosis of kidney transplant biopsies requires an expert pathologist using Banff guidelines to interpret features. Some problematic guidelines require experts to use professional…
2019 American Transplant Congress
Molecular Features of Rejection in Endobronchial Mucosa Biopsies
M. D. Parkes, K. M. Halloran, J. Chang, P. F. Halloran and The INTERLUNG Study Group
University of Alberta, Edmonton, AB, Canada
*Purpose: Mucosal biopsies are safer and easier to collect than transbronchial biopsies (TBB) but cannot be assessed histologically for lung rejection. Reliable assessment would change…
2019 American Transplant Congress
Impact of Belatacept Conversion on Renal Function, Histology and Gene Expression in Kidney Transplant Patients with Interstitial Fibrosis and Tubular Atrophy
G. Gupta¹, D. Kumar¹, M. Raynaud², A. Loupy², J. Chang³, J. Reeve³, P. Sanghi¹, I. Yakubu¹, L. Kamal¹, M. Levy¹, C. Bhati¹, P. Kimball¹, A. King¹, H. Massey¹, P. Halloran³
¹Virginia Commonwealth University, Richmond, VA, ²Paris Transplant Group, Paris, France, ³Alberta Transplant Genomics Applied Centre, Edmonton, AB, Canada
*Purpose: Prior studies on the conversion of tacrolimus (Calcineurin Inhibitor; CNI) to belatacept have been limited in scope due to either the absence of, post-conversion…
2019 American Transplant Congress
Immunomics of Renal Allograft Acute T-Cell Mediated Rejection Biopsies of Tacrolimus- and Belatacept- Treated Patients
M. van der Zwan¹, C. C. Baan¹, R. C. Colvin², R. N. Smith², R. A. White², D. N. Ndishabandi², A. L. Nigg¹, T. P. van den Bosch¹, G. N. de Graav¹, M. C. Clahsen-van Groningen¹, D. A. Hesselink¹
¹Internal Medicine, Division Nephrology and Transplantation, Erasmus MC, Rotterdam, Netherlands, ²Pathology, Massachusetts General Hospital/Harvard Medical School, Boston, MA
*Purpose: Belatacept-based therapy in kidney transplant recipient has been shown to increase long-term renal allograft and patient survival compared with calcineurin inhibitor based therapy, however…
2019 American Transplant Congress
Dosing Requirements Of Astagraf Xl In African American Kidney Transplant Recipients Converted From Twice-daily Tacrolimus (aaaktrs)
J. N. Fleming, M. A. Posadas-Salas, D. J. Taber
Medical University of South Carolina, Charleston, SC
*Purpose: Pharmacokinetics from clinical studies demonstrated similar drug exposure (AUC) and correlation between trough (C0) and AUC between tacrolimus IR and Astagraf XL, which led…