Articles tagged "Gene expression"

2019 American Transplant Congress
The Changing Face of T Cell-Mediated Rejection
A. Perkowska-Ptasinska and INTERCOMEX investigators¹, P. F. Halloran²
¹Medical University of Warsaw, Warsaw, Poland, ²Alberta Transplant Applied Genomics Centre, Edmonton, AB, Canada
*Purpose: We studied how the problem of TCMR in indication biopsies was changing with time by exploring features of 138 biopsies with molecular TCMR in…
2019 American Transplant Congress
Involvement of Genetic Variation and Related Gene Expression Changes on Acute Rejection in Renal Transplant Recipients
H. Hwang¹, J. Seo¹, J. Kim¹, Y. Kim¹, J. Moon¹, K. Jeong¹, C. Kim², J. Park³, B. Chung⁴, Y. Kim⁵, S. Lee¹
¹Division of Nephrology, Department of Internal Medicine, College of Medicine, Kyung Hee University, Seoul, Korea, Republic of, ²Division of Nephrology, Department of Internal Medicine, Kyungpook National University Hospital, Daegu, Korea, Republic of, ³Department of Surgery, Samsung Medical Center, Seoul, Korea, Republic of, ⁴Division of Nephrology, Department of Internal Medicine, The Catholic University of Korea, Seoul, Korea, Seoul, Korea, Republic of, ⁵Division of Nephrology, Department of Internal Medicine, College of Medicine, Inje University, Pusan, Korea, Republic of
*Purpose: Acute rejection (AR) is one of the major risk factors for renal allograft loss. The effect of genetic factors on the transplant outcomes has…
2019 American Transplant Congress
Relationship between HLA-DPB1 Gene Expression in Pre-Implantation Kidney Biopsies, Rs9277534 Polymorphism, and Late Graft Function
K. L. Mine¹, T. B. Mourão¹, C. Felipe², G. F. Rampim¹, J. O. Medina-Pestana², H. Tedesco-Silva², M. Gerbase-DeLima¹
¹Immunogenetics Institute, Associação Fundo de Incentivo a Pesquisa - AFIP, Sao Paulo, SP, Brazil, ²Hospital do Rim, Sao Paulo, SP, Brazil
*Purpose: A previous gene expression microarray study in pre-implantation biopsies (PIB) from kidneys of adult deceased donors (D) showed association between high class II HLA…
2019 American Transplant Congress
RNA Splicing and Lineage Progression: Identification of an Integrated Splicing Pattern in Endodermal Lineage Progression
J. Fair¹, N. Liu¹, S. G. Widen², W. S. Fagg¹
¹Surgery, University of Texas Medical Branch, Galveston, TX, ²Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX
*Purpose: Epigenetic states such as histone modifications and DNA methylation that influence transcription are well-characterized regulators of stem cell pluripotency and differentiation to specific lineages.…
2019 American Transplant Congress
Urinary Cell mRNA Sequencing Profiles of Kidney Allograft Recipients Mirror Intragraft Events and Demonstrate Enrichment for Immune Cells
M. Lubetzky¹, A. Verma², H. Yang¹, T. Muthukumar¹, S. Salvatore¹, J. Lee¹, D. Dadhania¹, S. Seshan¹, F. Mueller¹, O. Elemento², M. Suthanthiran¹
¹Division of Nephrology, Weill Cornell, New York, NY, ²Institute for Computational Biomedicine, Weill Cornell, New York, NY
*Purpose: Urinary cell mRNA profiling to interrogate kidney allograft status is based on the premise that the allograft can function as an in-vivo flow cytometer…
2019 American Transplant Congress
Subtyping Of Histologically Stable Kidney Transplants In Molecular Meta-analysis
D. Rychkov¹, T. Sigdel², J. Liberto², M. Sarwal², M. Sirota³
¹ICHS, Department of Surgery, UCSF, San Francisco, CA, ²Department of Surgery, UCSF, San Francisco, CA, ³ICHS, UCSF, San Francisco, CA
*Purpose: Histologically stable allografts have been shown to have molecular inflammation by prior gene expression studies. Nevertheless, there are no methodologies in place to sub-group…
2019 American Transplant Congress
Gene Expression Profiling for Cardiac Transplant Recipients: Results from the Outcomes AlloMap® Registry
S. Hall¹, G. Ewald², P. Berman³, A. Kao⁴, T. Wolf-Doty⁵, J. Yee⁵, J. Kobashigawa⁶
¹Baylor University Medical Center, Dallas, TX, ²Washington University, St. Louis, MO, ³Tampa General Hospital, Tampa, FL, ⁴Saint Luke's Mid America Heart Institute, Kansas City, MO, ⁵CareDx, Brisbane, CA, ⁶Cedars-Sinai Medical Center, Los Angeles, CA
*Purpose: Transplant registries may facilitate collection of comprehensive and unbiased real-world data on short and long-term outcomes of patients. We share the results of patients…
2019 American Transplant Congress
Pluripotent Stem Cell Differentiation: Delaying Forward Differentiation Signals Maintains Definitive Endoderm Phenotype for Cellular Transplantation
J. Fair¹, M. M. Shoja¹, N. Liu¹, I. Patrikeev², M. Motamedi², W. S. Fagg¹
¹Surgery, University of Texas Medical Branch, Galveston, TX, ²Opthalmology and Visual Sciences, University of Texas Medical Branch, Galveston, TX
*Purpose: Endodermal precursors (EP), derived in vitro from mouse pluripotent stem cell (PSC) sources, are capable of clinically useful engraftment in the unmodified (quiescent) liver…
2019 American Transplant Congress
The Complexity of T Cell-Mediated Rejection Scenarios: Identifying BK in Biopsies with TCMR
P. F. Halloran and The INTERCOMEX Investigators¹, G. Gupta², J. Reeve³
¹University of Alberta, Edmonton AB, AB, Canada, ²Virginia Commonwealth University, Richmond, VA, ³Alberta Transplant Applied Genomics Centre, Edmonton, AB, Canada
*Purpose: Some transplanted kidneys have concomitant T cell-mediated rejection (TCMR) and BK virus nephropathy (BK). The histologic diagnosis in BK sometimes overlooks the TCMR component,…
2019 American Transplant Congress
Cell Coding Transcriptomic Analysis Of Serial Blood And Biopsy Samples In A Large Cohort Of Kidney Transplant Recipients
W. Zhang¹, Z. Yi¹, Z. Sun¹, K. Keung², C. Wei¹, C. Xi¹, S. Farouk¹, M. Menon¹, P. Cravedi¹, P. O'Connell², B. Murphy¹
¹Icahn School of Medicine at Mount Sinai, New York, NY, ²The University of Sydney, Sydney, Australia
*Purpose: Whole blood and graft biopsy RNA expression analyses are limited by the heterogeneous cell composition of the samples. Cell coding is an analytical tool…