Articles tagged "Gene expression"

2019 American Transplant Congress
Molecular Features of Rejection in Endobronchial Mucosa Biopsies
M. D. Parkes, K. M. Halloran, J. Chang, P. F. Halloran and The INTERLUNG Study Group
University of Alberta, Edmonton, AB, Canada
*Purpose: Mucosal biopsies are safer and easier to collect than transbronchial biopsies (TBB) but cannot be assessed histologically for lung rejection. Reliable assessment would change…
2019 American Transplant Congress
Impact of Belatacept Conversion on Renal Function, Histology and Gene Expression in Kidney Transplant Patients with Interstitial Fibrosis and Tubular Atrophy
G. Gupta¹, D. Kumar¹, M. Raynaud², A. Loupy², J. Chang³, J. Reeve³, P. Sanghi¹, I. Yakubu¹, L. Kamal¹, M. Levy¹, C. Bhati¹, P. Kimball¹, A. King¹, H. Massey¹, P. Halloran³
¹Virginia Commonwealth University, Richmond, VA, ²Paris Transplant Group, Paris, France, ³Alberta Transplant Genomics Applied Centre, Edmonton, AB, Canada
*Purpose: Prior studies on the conversion of tacrolimus (Calcineurin Inhibitor; CNI) to belatacept have been limited in scope due to either the absence of, post-conversion…
2019 American Transplant Congress
Immunomics of Renal Allograft Acute T-Cell Mediated Rejection Biopsies of Tacrolimus- and Belatacept- Treated Patients
M. van der Zwan¹, C. C. Baan¹, R. C. Colvin², R. N. Smith², R. A. White², D. N. Ndishabandi², A. L. Nigg¹, T. P. van den Bosch¹, G. N. de Graav¹, M. C. Clahsen-van Groningen¹, D. A. Hesselink¹
¹Internal Medicine, Division Nephrology and Transplantation, Erasmus MC, Rotterdam, Netherlands, ²Pathology, Massachusetts General Hospital/Harvard Medical School, Boston, MA
*Purpose: Belatacept-based therapy in kidney transplant recipient has been shown to increase long-term renal allograft and patient survival compared with calcineurin inhibitor based therapy, however…
2019 American Transplant Congress
Dosing Requirements Of Astagraf Xl In African American Kidney Transplant Recipients Converted From Twice-daily Tacrolimus (aaaktrs)
J. N. Fleming, M. A. Posadas-Salas, D. J. Taber
Medical University of South Carolina, Charleston, SC
*Purpose: Pharmacokinetics from clinical studies demonstrated similar drug exposure (AUC) and correlation between trough (C0) and AUC between tacrolimus IR and Astagraf XL, which led…
2019 American Transplant Congress
The Molecular Features of Chronic Lung Allograft Dysfunction (CLAD) in Transbronchial and Endobronchial Mucosa Biopsies
M. D. Parkes¹, K. M. Halloran¹, J. Chang², P. F. Halloran and the INTERLUNG study group¹
¹University of Alberta, Edmonton, AB, Canada, ²ATAGC, University of Alberta, Edmonton, AB, Canada
*Purpose: Chronic lung allograft dysfunction (CLAD), the major long-term problem in lung transplantation, requires new strategies for prevention and management. However, CLAD is infrequently diagnosed…
2019 American Transplant Congress
Using Ensembles of Machine Learning Classifiers to Maximize the Accuracy and Stability of Molecular Biopsy Interpretation
J. Reeve¹, K. S. Madill-Thomsen², P. F. Halloran and the INTERCOMEX Study Group³
¹University of Alberta, Edmonton, AB, Canada, ²Alberta Transplant Applied Genomics Centre, University of Alberta, Edmonton AB, AB, Canada, ³University of Alberta, Edmonton AB, AB, Canada
*Purpose: The Molecular Microscope diagnostic system (MMDx), based on microarray gene expression, uses ensembles of machine learning classifiers rather than single genes, gene sets, or…
2019 American Transplant Congress
Single Cell RNA-seq Analysis of Antibody-Mediated Rejection Reveals Endothelial – Pericyte NOTCH Signaling
A. F. Malone, A. Chadha, H. Wu, S. Ramakrishnan, E. Donnelly, B. D. Humphreys
Washington University School of Medicine, Saint Louis, MO
*Purpose: To precisely define endothelial cell responses in AMR we performed comprehensive single cell RNA-seq on a human kidney biopsy with AMR.*Methods: Single cell libraries…
2019 American Transplant Congress
Role of Mitochondria, Metabolism and Inflammation: Pathways Influencing Short Term Outcomes Post-Renal Transplantation
S. Bontha¹, A. Bajwa¹, G. Lorenzo², J. Eason³, D. Maluf⁴, V. Mas⁵
¹Surgery, UTHSC, Memphis, TN, ²Northwestern University, Chicago, IL, ³Director of Transplant Institute UTHSC, Memphis, TN, ⁴UTHSC, Memphis, TN, ⁵Director of Transplant Research Institute, Memphis, TN
*Purpose: Preconditioning of donor kidneys and clinical assessment for need of dialysis early post-transplant vary per each case and are crucial for improving transplant outcomes.…
2019 American Transplant Congress
Discovery and Validation of Blood Genomic Signatures of Acute Rejection in Liver Transplant Recipients
J. Levitsky¹, N. Bridges², J. Odim², M. Brown², D. Ikle³, B. Armstrong³, A. Demetris⁴, S. Asrani⁵, G. Klintmalm⁵, T. Schiano⁶, A. Moss⁷, K. Chavin⁸, C. Miller⁹, M. Kandpal¹⁰, L. Zhao¹⁰, S. Kurian¹¹, M. Abecassis¹⁰
¹Div of Gastroenterology & Comprehensive Tx Cnt, Chicago, IL, ²NIAID, Bethesda, MD, ³Rho, Chapel Hill, NC, ⁴UPMC, Pittsburgh, PA, ⁵Baylor, Dallas, TX, ⁶Mount Sinai, New York, NY, ⁷Mayo Arizona, Phoenix, AZ, ⁸Case Western, Cleveland, OH, ⁹Cleveland Clinic, Cleveland, OH, ¹⁰Comprehensive Transplant Center, Chicago, IL, ¹¹Scripps, San Diego, CA
*Purpose: Biomarker profiles diagnostic of acute rejection (AR) could enhance the management of liver transplant (LT) recipients. Our aim was to discover and validate blood…
2019 American Transplant Congress
Comparing Antibody-Mediated Rejection with or without Detectable DSA: Similar Pathology and Similar Poor Prognosis
G. Einecke¹, J. Reeve², P. F. Halloran³
¹Dpt. of Nephrology, Hannover Medical School, Hannover, Germany, ²Dpt. of Laboratory Medicine and Pathology and Alberta Transplant Applied Genomics Centre, University of Alberta, Edmonton, AB, Canada, ³Dpt. of Nephrology and Alberta Transplant Applied Genomics Centre, University of Alberta, Edmonton, AB, Canada
*Purpose: Banff guidelines require the detection of donor-specific antibodies (DSA) in addition to microvascular inflammation (MVI) for a diagnosis of antibody-mediated rejection (ABMR), but MVI…