2019 American Transplant Congress
Targeted Epitope Mutagenesis as a Strategy to Decrease Xenoantibody Binding
University of Alabama-Birmingham, Birmingham, AL
*Purpose: Xenotransplantation, the use of genetically-modified pig organs for transplantation, has been limited by antibody-mediated rejection. The generation of a three-antigen knockout pig provided a…2019 American Transplant Congress
α -1,3-galactosyltransferase Knockout Porcine Tissue And Organ May Reduce The Risks Of Antibody Mediated Rejection Via Less Rhesus Igm Cross-reactivity.
Duke Transplant Center, Durham, NC
*Purpose: Previously, we observed that sera from rhesus macaques contained preformed antibodies against wild type (WT) swine splenocytes. In the context of xenotransplantation, preformed xenoantibodies…2019 American Transplant Congress
Impact Of Delayed Kidney Transplantation On Positive Crossmatch Liver Kidney Transplantation
Department of Surgery, Indiana University Hospital, Indianapolis, IN
*Purpose: Kidney transplantation in sensitized patients is associated with an increased immunological risk. Significant decrease in circulating anti-HLA donor specific antibodies (DSA) has been observed…2018 American Transplant Congress
Clinical Implications of Positive Crossmatch in Liver Retransplants
Surgery, University of Cincinnati, Cincinnati, OH.
BackgroundLiver transplant has been performed successfully regardless of a positive crossmatch. However, the clinical implications of a positive crossmatch in liver retransplants have not been…2018 American Transplant Congress
Evaluation of FlowDSA Crossmatch for Detection of Donor-Specific HLA Antibodies
Background: Donor-specific HLA antibodies (DSA) are detected by different crossmatch techniques. Flow cytometric crossmatching (FCXM) is sensitive method, but it is subject to false (+)…2018 American Transplant Congress
False-Positive Flow-Cytometric T Cell Crossmatch Associated with Blood Type Antigen Expressed on Lymphocytes in ABO Incompatible Kidney Transplantation
Background: Flow-cytometric T cell crossmatch (FTXM) uses live donor lymphocytes to detect anti-donor-human leukocyte antigen (HLA) IgG antibodies. Therefore, the results can be affected by…2018 American Transplant Congress
Can We Expect the “Unexpected”?
Northwestern University, Chicago.
A known, seemingly paradoxical, phenomenon of the HLA system is the high overall degree of homology among different HLA alleles, despite vast polymorphism in the…2018 American Transplant Congress
A Novel Endothelial Cell Based Complement Dependent Cytotoxicity Test in Kidney Transplantation
Introduction:Relevant alloantibodies in kidney transplantation comprise anti-HLA antibodies, blood group antibodies (ABO incompatible) and anti-endothelial cell antibodies (AECA) and can initiate antibody-mediated rejection (ABMR). ABMR…2018 American Transplant Congress
Flow DSA-XM: Better Crossmatch or Just Different Problems?
Surgery, VCUHealth Systems, Richmond, VA.
Background. Despite the positive impact of flow crossmatching(FCXM) upon alloantibody detection, its inability to distinguish anti-Class I from Class II or non-HLA from HLA-antibodies remain…2017 American Transplant Congress
Can Pre-Treatment CDC Titres Predict Graft Outcomes in HLA Incompatible Transplants?
Introduction: Complement Dependent Cytotoxic (CDC) (non-augmented) cross matching and Flow cytometric (FC) cross matching are two commonly used techniques to identify anti- Human Leucocyte Antigen…
American Transplant Congress abstracts