Articles tagged "Flowcytometry crossmatching"

2022 American Transplant Congress
Desensitization Protocol Consisting of High-Dose Intravenous Immunoglobulin, Plasmapheresis, and Rituximab in Highly Sensitized Kidney Transplant Recipients
M. Okada, T. Hiramitsu, T. Tomosugi, K. Futamura, S. Narumi, N. Goto, Y. Watarai
Nagoya Daini Red Cross Hospital, Nagoya, Japan
*Purpose: In HLA incompatible kidney transplantation, donor-specific HLA antibody often causes antibody-mediated rejection (AMR). To prevent AMR, we adopted a preoperative desensitization protocol including high-dose…
2022 American Transplant Congress
How Does Anti-CD38 Antibody Treatment (Daratumumab) Impact Immunological Assessment in Solid Organ Transplantation?
S. Ho¹, K. Putnam², C. Peiter², W. Herczyk², J. Gerlach³, E. Campagnaro⁴, Y. Lu⁴, K. Woodside⁴, M. Cusick⁴
¹Gift of Hope Organ & Tissue Donor Network, Itasca, IL, ²Gift of Life Michigan, Ann Arbor, MI, ³Michigan State University, East Lansing, MI, ⁴University of Michigan Medicine, Ann Arbor, MI
*Purpose: We report the first case of Dara interference of routine pre-transplant (Tx) crossmatch (XM) repeatedly causing aberrant false positives which inadvertently delayed Tx for…
2021 American Transplant Congress
Deceased Donor Transplantation Across Positive Flow Crossmatch – A Single Center Experience
M. Campara, A. Lichvar, J. Benken, S. Patel, C. Muran, D. Pierce, I. Tang, P. Di Cocco, J. Almario Alvarez, M. Spaggiari, E. Benedetti, I. Tzvetanov
University of Illinois at Chicago, Chicago, IL
*Purpose: Transplantation across a positive flow crossmatch (+FXM) has been established as a lifesaving measure in the living donor setting. Time required for desensitization limits…
2020 American Transplant Congress
Outcomes of Post-Transplant Desensitization in High Immunological Risk Deceased Donor Kidney Transplant Recipients
D. Kumar, C. Song, L. Kamal, I. Moinuddin, M. Levy, C. Bhati, A. King, P. Kimball, G. Gupta
Virginia Commonwealth University, Richmond, VA
*Purpose: The kidney allocation system (KAS) was changed in 2015 to increase access to transplants for highly sensitized patients. However, recent data suggests that even…
2020 American Transplant Congress
The Economic Impact of National Standardization of Virtual Crossmatch Usage in Kidney Transplantation
J. E. Stanton¹, L. M. Rebellato², H. Pournik¹, J. E. Tuttle-Newhall¹, H. Jones¹, W. Irish¹, D. B. Leeser¹
¹Department of Surgery, East Carolina University, The Brody School of Medicine, Greenville, NC, ²Department of Pathology, East Carolina University, The Brody School of Medicine, Greenville, NC
*Purpose: The implementation of virtual crossmatching (VXM) into conventional protocols may reduce expenditures associated with organ allocation.*Methods: A single center retrospective analysis of the total…
2020 American Transplant Congress
Preformed Donor Specific Antibody and Mfi-Dependent Effects in Simultaneous Liver-Kidney Transplantation
V. Fleetwood, C. Papageorge, D. Aufhauser, G. Leverson, B. Welch, L. Hidalgo, D. Foley, Y. Yankol, A. D'Alessandro, D. Al-Adra, R. Redfield, J. Mezrich
Department of Transplantation, University of Wisconsin, Madison, WI
*Purpose: Preformed donor specific antibody to HLA Class II (pDSA) is suggested to be an independent risk factor for mortality in simultaneous liver and kidney…
2020 American Transplant Congress
Influence of Human Leukocyte Antigen Status and Gender of Positive Crossmatch Patients on Long-Term Survival Outcomes of Antibody Incompatible Renal Transplantation
N. R. Macha¹, A. Abimbola¹, N. Khovanova², D. Briggs³, N. Krishnan⁴
¹CIRAL, Coventry University, Coventry, United Kingdom, ²School of Engineering, University of Warwick, Warwickshire, United Kingdom, ³Director, Tissue Typing Lab, NHSBT, Birmingham, United Kingdom, ⁴Consultant Transplant Nephrologist and Visiting Professor, CIRAL, University Hospital Coventry & Warwickshire, Coventry, United Kingdom
*Purpose: Human Leukocyte Antigen (HLA) sensitization is a significant obstacle which limits the use of renal transplantation, but it serves as a therapeutic option for…
2020 American Transplant Congress
Assessment of Rapid Optimized Flow Cytometric Crossmatch (FCXM) Assay with Luminex Class I Single Antibody Test
J. Yoo¹, S. Lee², J. Jang³, A. Choi², E. Oh¹
¹Department of Laboratory Medicine, Catholic University of Korea, Seoul, Korea, Republic of, ²Department of Laboratory Medicine, Seoul St. Mary's Hospital, Seoul, Korea, Republic of, ³Department of Biomedical Science, Graduate School, Catholic University of Korea, Seoul, Korea, Republic of
*Purpose: Flow cytometric crossmatch test (FCXM) is critical to detect donor-specific antibodies (DSA) in transplant patients. Rapid, simple and sensitive FCXM procedure can improve HLA…
2019 American Transplant Congress
Impact Of Delayed Kidney Transplantation On Positive Crossmatch Liver Kidney Transplantation
Z. Rokop, C. Kubal, B. Ekser, R. Mangus, J. Fridell, P. Mihaylov, W. Goggins
Department of Surgery, Indiana University Hospital, Indianapolis, IN
*Purpose: Kidney transplantation in sensitized patients is associated with an increased immunological risk. Significant decrease in circulating anti-HLA donor specific antibodies (DSA) has been observed…
2019 American Transplant Congress
The Effect Of Exclusive Virtual Crossmatch To Allocate Deceased Donor Kidney Transplant On Cold Ischemia Time And Acute Rejection
E. Abuhelaiqa¹, M. Alkadi¹, S. Thappy¹, S. Saleh², J. Mahmoud³, M. Jarman³, O. Fituri¹, M. Elkhalifa⁴, J. Wadström³, H. Al-Malki¹
¹Medicine-Nephrology, Hamad Medical Corporation, Doha, Qatar, ²Histocompatibility & Immunogenetics Laboratory,, Hamad Medical Corporation, Doha, Qatar, ³Surgery-Transplant, Hamad Medical Corporation, Doha, Qatar, ⁴Histocompatibility & Immunogenetics Laboratory, Hamad Medical Corporation, Doha, Qatar
*Purpose: Virtual crossmatch (VXM) using Luminex single antigen bead has significantly improved prediction of an actual negative crossmatch. A negative crossmatch is typically required for…