ATC Abstracts

American Transplant Congress abstracts

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Articles tagged "FACS analysis"

  • 2016 American Transplant Congress

    The Bacterial Enzyme IdeS, Inhibits HLA Antibody-Mediated NK Cell Activation and Cell Cytotoxicity (ADCC) In Vitro.

    S. Ge, M. Chu, J. Choi, S. Jordan, M. Toyoda.

    Transplant Immunology Laboratory, Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles.

    Background: Antibody-mediated rejection (ABMR) is a major obstacle for successful transplantation in HLA-sensitized patients. ADCC is one of the primary mechanisms for ABMR. IdeS cleaves…
  • 2016 American Transplant Congress

    An Improved Flow Cytometry Based Platelet Counting in Xeno Perfusion Models.

    S. Sendil,1 G. Braileanu,1 W. Sun,1 W. Hassanein,1 D. Parsell,1 H. Crum,2 L. Burdorf,1 R. Pierson,1 A. Azimzadeh.1

    1Department of Surgery, University of Maryland School of Medicine, Baltimore, MD; 2VA Medical Center, Baltimore, MD.

    Aim: Human or baboon platelet sequestration and activation occurs during pig organ xenograft perfusion; its mechanism remains poorly understood. In the context of a regimen…
  • 2016 American Transplant Congress

    Polyfunctionality of CD8 T Cell Responses to Cytomegalovirus in Transplant Patients.

    L. Higdon,1 K. Margulies,2 J. Maltzman.1,3

    1Stanford University, Palo Alto; 2University of Pennsylvania, Philadelphia; 3Veterans Administration Medical Center, Palo Alto.

    Background: Cytomegalovirus (CMV) infection causes significant morbidity including allograft damage in transplant patients. Primary infection with CMV is rapidly resolved in healthy individuals; memory T…
  • 2015 American Transplant Congress

    Evaluation of Natural Killer Cell Activation by NK Cell-Specific Interferon-Gamma-Releasing Assay

    J. Ryu,1 K. Park,1 Y. Kim,2 E.-J. Oh.2

    1Department of Biomedical Science, Graduate School, The Catholic University of Korea, Seoul, Republic of Korea; 2Laboratory Medicine, Seoul St. Mary's Hospital, the Catholic University of Korea, Seoul, Republic of Korea.

    Introduction: Although NK cell activity (NKA) has a variety of measuring markers including chromiun release assay and flowcytometry-based cytolysis, limitations such as radioactivity and lack…
  • 2015 American Transplant Congress

    Biomarker Panel Profile for Identification of High-Risk Patients After Heart Transplantation

    M. Barten,1 M.-T. Dieterlen,2 J. Garbade,2 F. Mohr.2

    1Cardiovascular Surgery, Universisty Heart Center Hamburg, Hamburg, Germany; 2Cardiac Surgery, University Leipzig Heart Center, Leipzig, Germany.

    Background: The first year after cardiac transplantation is critical for transplant survival and long-term outcome. Identification of suitable biomarkers for detection of rejection processes is…
  • 2015 American Transplant Congress

    Transient Lymphopenia Breaks Costimulatory Blockade-Based Transplant Tolerance and Initiates Allograft Rejection

    S. Iida,1 T. Suzuki,2 K. Tanabe,1 A. Valujskikh,3 R. Fairchild,3 R. Abe.2

    1Urology, Tokyo Women's Medical Univ, Tokyo, Japan; 2Immunology, Science University of Tokyo, Chiba, Japan; 3Immunology, Cleveland Clinic, Cleveland, OH.

    【Background and Objective】: Lymphopenia is induced by lymphoablative therapies and chronic viral infections. Lymphopenia is observed in some pathological and physiological processes, including chronic viral…
  • 2015 American Transplant Congress

    Comprehensive Study of 3 Nomenclatures to Discriminate CD8 Subsets in Healthy Volunteers and in Kidney Transplant Recipients

    M. Yap,1,3 G. Tilly,1,3 M. Giral,1,2 S. Brouard,1,2 N. Degauque.1,2

    1UMR 1064, INSERM, Nantes, France; 2ITUN, CHU de Nantes, Nantes, France; 3Faculté de Médecine, Université de Nantes, Nantes, France.

    Objective. Historical classification to distinguish central memory (CM), naïve, effector memory (EM), and terminally-differentiated effector memory (TEMRA) CD8 T cells is based on CD45RA and…
  • 2015 American Transplant Congress

    Intra-Renal Cytokine Measurement Detected During Kidney Allograft Biopsies Portend Severity of Histological Injury

    N. Leca, S. Anderson, K. Muczynski.

    Division of Nephrology, University of Washington, Seattle, WA.

    Histological changes observed on renal allograft biopsies, although considered the current standard for evaluation of allograft injury, lack specificity and disease activity assessment. We devised…
  • 2015 American Transplant Congress

    Characterization of a Nude Mouse Model of Allosensitization Conditioned by Adoptive Transfer of Follicular T Helper Cells

    I. Kim, N.-N. Chai, S. Jordan, A. Klein, G. Wu.

    Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA.

    Background Follicular T helper (Tfh) cells play a critical role in follicular B cell activation and maturation during antibody responses to allograft. Here we report…
  • 2015 American Transplant Congress

    Kidney Plasmacytoid Dendritic Cells Display Distinct Cell Surface Markers and May Explain the Induction of Tolerance by Kidney Allografts

    N. Oh, M. Tonsho, D. Ndishabandi, R. Colvin, J. Madsen, A. Alessandrini.

    Massachusetts General Hospital, Boston, MA.

    Achieving tolerance in heart allografts is an important area of research as the need for heart transplantations increase. Recent studies in non-human primates (NHPs) have…
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