ATC Abstracts

American Transplant Congress abstracts

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Articles tagged "FACS analysis"

  • 2016 American Transplant Congress

    Impact of Immunosuppressants on Inducing Regulatory T Cells in Human Allo-Immune Responses.

    Y. Tanaka, H. Ohdan.

    Transplant Surgery, Hiroshima University, Hiroshima, Japan.

    A better understanding of cytokine signaling pathways involving transcription factors of the STAT family in T cells responding to allostimulation will improve interpretation of immune-regulatory…
  • 2016 American Transplant Congress

    Plasma Endothelial Microvesicle Counts Indicate Allograft Rejection Following Kidney Transplantation.

    D. Zecher,1 A. Cumpelik,2 B. Ruhland,1 B. Banas,1 H. Hopfer,3 M. Dickenmann,4 J. Schifferli.2

    1Nephrology, Regensburg University Hospital, Regensburg, Germany; 2Biomedicine, Basel University Hospital, Basel, Switzerland; 3Pathology, Basel University Hospital, Basel, Switzerland; 4Nephrology, Basel University Hospital, Basel, Switzerland.

    Background: The release of surface-derived microvesicles (MV) is a common phenomenon of cellular activation and occurs specifically as a consequence of terminal complement complex assembly…
  • 2016 American Transplant Congress

    The Bacterial Enzyme IdeS, Inhibits HLA Antibody-Mediated NK Cell Activation and Cell Cytotoxicity (ADCC) In Vitro.

    S. Ge, M. Chu, J. Choi, S. Jordan, M. Toyoda.

    Transplant Immunology Laboratory, Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles.

    Background: Antibody-mediated rejection (ABMR) is a major obstacle for successful transplantation in HLA-sensitized patients. ADCC is one of the primary mechanisms for ABMR. IdeS cleaves…
  • 2015 American Transplant Congress

    Immune Phenotyping of Peripheral Blood Samples from the CTOT-10 Belatacept Study

    Y. Suessmuth,1 L. Stempora,1 B. Johnson,1 J. Cheeseman,1 Y. Morrison,4 N. Bridges,4 D. Ikle,5 S. Tomlanovich,3 P. Stock,3 R. Mannon,2 K. Newell,1 C. Larsen,1 A. Mehta.1

    1Emory Transplant Center, Atlanta; 2University of Alabama, Birmingham; 3University of California San Francisco, San Francisco; 4NIAID/ NIH, Bethesda; 5Rho, Chapel Hill, NC.

    The purpose of the CTOT-10 study is to evaluate belatacept as maintenance therapy, while minimizing calcineurin inhibitors and corticosteroids in renal transplantation. We designed mechanistic…
  • 2015 American Transplant Congress

    Single Cell Phospho-Protein Analysis Demonstrates That Immunosuppressive Drugs Inhibit MAPK and mTOR Signaling Molecules in Primary Monocytes After Transplantation

    N. Kannegieter, D. Hesselink, R. Kraaijeveld, G. de Graav, M. Betjes, W. Weimar, C. Baan.

    Nephrology and Transplantation, Dept. of Internal Medicine, Erasmus MC, Rotterdam, Netherlands.

    Monocytes have been identified as key players driving rejection processes. It is therefore surprising that there are almost no data available about the impact of…
  • 2015 American Transplant Congress

    Impact of CXCR4/CXCL12 Blockade on Normal Plasma Cells In Vivo

    N. Moore,1 M. Moreno Gonzales,2 M. Stegall.1

    1Department of Surgery, Division of Transplant Surgery and Department of Immunology, Mayo Clinic, Rochester, MN; 2Department of Surgery, Division of Transplant Surgery, Mayo Clinic, Rochester, MN.

    Receptor/ligand interactions in stromal microenvironments play important roles in the localization, development and survival of normal antibody-secreting plasma cells. The goal of the current study…
  • 2015 American Transplant Congress

    Alterations in Peripheral Blood B Cell Subpopulations in Kidney Transplant Recipients With DSA

    B. Ramaswami,1 R. Ippolito,2 B. Elinoff,1 M. Marrari,3 K. Hadi,1 C. Macedo,1 D. Metes,1 A. Shields,4 R. Alloway,5 A. Zeevi,3 F. Lakkis,1 E. Woodle,4 G. Chalasani.1,2

    1Surgery & STI(1), Medicine(2), Pathology(3), University of Pittsburgh, Pittsburgh; 2Surgery(4) & Medicine(5), University of Cincinnati College of Medicine, Cincinnati.

    Peripheral blood B cell subpopulations were assessed over time (1, 3, 6, 9, and 12 months) in living donor kidney transplant recipients undergoing thymoglobulin (n…
  • 2015 American Transplant Congress

    Evaluation of Natural Killer Cell Activation by NK Cell-Specific Interferon-Gamma-Releasing Assay

    J. Ryu,1 K. Park,1 Y. Kim,2 E.-J. Oh.2

    1Department of Biomedical Science, Graduate School, The Catholic University of Korea, Seoul, Republic of Korea; 2Laboratory Medicine, Seoul St. Mary's Hospital, the Catholic University of Korea, Seoul, Republic of Korea.

    Introduction: Although NK cell activity (NKA) has a variety of measuring markers including chromiun release assay and flowcytometry-based cytolysis, limitations such as radioactivity and lack…
  • 2015 American Transplant Congress

    Biomarker Panel Profile for Identification of High-Risk Patients After Heart Transplantation

    M. Barten,1 M.-T. Dieterlen,2 J. Garbade,2 F. Mohr.2

    1Cardiovascular Surgery, Universisty Heart Center Hamburg, Hamburg, Germany; 2Cardiac Surgery, University Leipzig Heart Center, Leipzig, Germany.

    Background: The first year after cardiac transplantation is critical for transplant survival and long-term outcome. Identification of suitable biomarkers for detection of rejection processes is…
  • 2015 American Transplant Congress

    Transient Lymphopenia Breaks Costimulatory Blockade-Based Transplant Tolerance and Initiates Allograft Rejection

    S. Iida,1 T. Suzuki,2 K. Tanabe,1 A. Valujskikh,3 R. Fairchild,3 R. Abe.2

    1Urology, Tokyo Women's Medical Univ, Tokyo, Japan; 2Immunology, Science University of Tokyo, Chiba, Japan; 3Immunology, Cleveland Clinic, Cleveland, OH.

    【Background and Objective】: Lymphopenia is induced by lymphoablative therapies and chronic viral infections. Lymphopenia is observed in some pathological and physiological processes, including chronic viral…
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