ATC Abstracts

American Transplant Congress abstracts

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Articles tagged "FACS analysis"

  • 2017 American Transplant Congress

    The Role of Anti-Neu5Gc IgM in Xenograft Antibody Mediated Rejection.

    W. Hassanein,1 G. Braileanu,1 L. Burdorf,1 W. Sun,1 B. French,1 C. Laird,1 S. Sendil,1 C. Ahn,3 D. Ayares,2 R. Pierson III,1 A. Azimzadeh.1

    1University of Maryland School of Medcine, Baltimore, MD; 2Revivicor, Blacksburg, VA; 3Seoul National University Hospital, Seoul, Korea

    Background:Acute antibody mediated rejection due to preformed antibodies present in human serum remains a hurdle to successful xenotransplantation. Here, we measured anti-Neu5Gc-IgM antibody titers in…
  • 2017 American Transplant Congress

    Effect of Two Anti-CD20 Abs on B Cell Depletion and Reconstitution in Non-Human Primates.

    W. Sun,1 N. O'Neill,1 T. Zhang,1 G. Braileanu,1 K. Reimann,2 R. Pierson,1 A. Azimzadeh.1

    1Dept. of Surgery, University of Maryland, School of Medicine, Baltimore, MD; 2MassBiologics, University of Massachusetts Medical School, Boston, MA

    Background: B lymphocytes play a central role in host immunity, including antibody elaboration and antigen presentation, and B-cell depletion is being explored to modulate alloimmunity.…
  • 2017 American Transplant Congress

    Reduced Expression of Membrane Complement Regulatory Protein CD59 on Leukocytes Following Lung Transplantation.

    L. Michielsen,1 K. Budding,2 D. Drop,2 E. van de Graaf,3 T. Kardol-Hoefnagel,2 M. Verhaar,1 A. van Zuilen,1 H. Otten.2

    1Department of Nephrology and Hypertension, University Medical Center Utrecht, Utrecht, Netherlands; 2Laboratory of Translational Immunology, University Medical Center Utrecht, Utrecht, Netherlands; 3Department Respiratory Medicine, University Medical Center Utrecht, Utrecht, Netherlands

    BackgroundCellular protection against unwanted complement activation is provided by expression of membrane-bound complement regulatory proteins including CD59. This protein prevents membrane attack complex formation and…
  • 2017 American Transplant Congress

    In Depth Characterization of the Innate Immune Response in Transplant Recipients with EBV Viremia.

    V. Ferreira, J. Batist, D. Kumar, S. Husain, A. Humar.

    UHN, Toronto, Canada

    Background:Infection with Epstein-Barr virus (EBV) manifests as a spectrum of disease ranging from asymptomatic viremia to PTLD. Innate immune cells such as monocytes/macrophages, dendritic cells…
  • 2016 American Transplant Congress

    Characterization of T Regulatory Type 1 (Tr1) Cells in Naïve and Transplanted Non-Human Primates.

    R. Yu, M. Tonsho, P. Spencer, S. Bernard-Stoecklin, G. Benichou, J. Madsen.

    Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA.

    BackgroundT regulatory Type 1 (Tr1) cells are peripheral CD4+FoxP3- regulatory T cells expressing CD49b and LAG-3 in mice and humans. Mouse Tr1 cells inhibit antigen-presenting…
  • 2016 American Transplant Congress

    Impact of Immunosuppressants on Inducing Regulatory T Cells in Human Allo-Immune Responses.

    Y. Tanaka, H. Ohdan.

    Transplant Surgery, Hiroshima University, Hiroshima, Japan.

    A better understanding of cytokine signaling pathways involving transcription factors of the STAT family in T cells responding to allostimulation will improve interpretation of immune-regulatory…
  • 2016 American Transplant Congress

    Plasma Endothelial Microvesicle Counts Indicate Allograft Rejection Following Kidney Transplantation.

    D. Zecher,1 A. Cumpelik,2 B. Ruhland,1 B. Banas,1 H. Hopfer,3 M. Dickenmann,4 J. Schifferli.2

    1Nephrology, Regensburg University Hospital, Regensburg, Germany; 2Biomedicine, Basel University Hospital, Basel, Switzerland; 3Pathology, Basel University Hospital, Basel, Switzerland; 4Nephrology, Basel University Hospital, Basel, Switzerland.

    Background: The release of surface-derived microvesicles (MV) is a common phenomenon of cellular activation and occurs specifically as a consequence of terminal complement complex assembly…
  • 2016 American Transplant Congress

    The Bacterial Enzyme IdeS, Inhibits HLA Antibody-Mediated NK Cell Activation and Cell Cytotoxicity (ADCC) In Vitro.

    S. Ge, M. Chu, J. Choi, S. Jordan, M. Toyoda.

    Transplant Immunology Laboratory, Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles.

    Background: Antibody-mediated rejection (ABMR) is a major obstacle for successful transplantation in HLA-sensitized patients. ADCC is one of the primary mechanisms for ABMR. IdeS cleaves…
  • 2016 American Transplant Congress

    An Improved Flow Cytometry Based Platelet Counting in Xeno Perfusion Models.

    S. Sendil,1 G. Braileanu,1 W. Sun,1 W. Hassanein,1 D. Parsell,1 H. Crum,2 L. Burdorf,1 R. Pierson,1 A. Azimzadeh.1

    1Department of Surgery, University of Maryland School of Medicine, Baltimore, MD; 2VA Medical Center, Baltimore, MD.

    Aim: Human or baboon platelet sequestration and activation occurs during pig organ xenograft perfusion; its mechanism remains poorly understood. In the context of a regimen…
  • 2016 American Transplant Congress

    Polyfunctionality of CD8 T Cell Responses to Cytomegalovirus in Transplant Patients.

    L. Higdon,1 K. Margulies,2 J. Maltzman.1,3

    1Stanford University, Palo Alto; 2University of Pennsylvania, Philadelphia; 3Veterans Administration Medical Center, Palo Alto.

    Background: Cytomegalovirus (CMV) infection causes significant morbidity including allograft damage in transplant patients. Primary infection with CMV is rapidly resolved in healthy individuals; memory T…
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