Articles tagged "FACS analysis"

2019 American Transplant Congress
B Cell Derived Exosomes Are Associated with Antibody Mediated Rejection in Kidney Transplant Recipients Desensitized for a Positive Cross-Match
D. Cucchiari¹, V. Tubita², M. J. Ramirez-Bajo², J. Rovira², F. E. Borràs³, L. Carreras-Planella³, A. Hertig⁴, F. Oppenheimer¹, F. Diekmann¹, I. Revuelta¹
¹Renal Transplant Unit, Hospital Clínic, Barcelona, Spain, ²Laboratori Experimental de Nefrologia i Trasplantament, IDIBAPS, Barcelona, Spain, ³REMAR-IVECAT group, IGTP, Badalona, Spain, ⁴Department of Nephrology and Kidney Transplantation, Hôpital Tenon, Paris, France
*Purpose: Kidney transplant recipients undergoing desensitization for a positive cross-match are at very high risk of developing Antibody-Mediated Rejection (AMR). Because of desensitization, peripheral circulating…
2019 American Transplant Congress
Dynamic Characterization of Cells in the Perfusate from Cold Ischemic Liver Grafts
R. J. de Vries, C. Pendexter, S. E. Cronin, B. Marques, J. F. Markmann, S. L. Stott, H. Yeh, M. Toner, K. Uygun, S. N. Tessier
Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA
*Purpose: Injury during preservation is a complex process that affects graft function. A deeper understanding and better prediction of liver viability are demanded to improve…
2019 American Transplant Congress
Novel Flow-Based Assay to Identify Allo-Specific T Cell Exhaustion
N. Tanimine¹, C. Rickert¹, K. Lee¹, K. Deng¹, N. Feeney¹, C. Leguern¹, B. Burrell², J. Markmann¹
¹Department of Surgery, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Immune Tolerance Network, Bethesda, MD
*Purpose: A recent, multi-center immunosuppression withdrawal trial in liver transplant recipients unexpectedly revealed time post-transplant as an important predictor for operational tolerance. We hypothesize that…
2019 American Transplant Congress
Extracorporeal Photopheresis for Specific Immune Tolerance Responses in Heart Transplant Recipients
M. J. Barten¹, K. Klaeske², J. Wittke², M. Borger², J. Garbade², S. Lehmann³, H. Reichenspurner¹, M. Dieterlen²
¹Cardiovascular Surgery, University Heart Center, Hamburg, Germany, ²Cardiac Surgery, University Heart Center Leipzig, Leipzig, Germany, ³Cardiacr Surgery, University Heart Center Leipzig, Leipzig, Germany
*Purpose: Extracorporeal photopheresis (ECP) treatment following heart transplantation (HTx) may induce transplant tolerance. A monitoring tool for successful ECP treatment would allow determination of patient-specific…
2019 American Transplant Congress
Effects of Thalidomide and Dexamethasone Co-Treatment on CD8⁺ T Cells
J. Kim¹, E. Kim², Y. Cho¹, H. Lee¹, M. Kim³, Y. Kim³, K. Huh³, B. Kim⁴
¹The Research Institute for Transplantation, Yonsei University College of Medicine, Seoul, Korea, Republic of, ²Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea, Republic of, ³Department of Transplantation Surgery, Yonsei University College of Medicine, Seoul, Korea, Republic of, ⁴Nephrology Division, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea, Republic of
*Purpose: Thalidomide (TM) is known to have anti-cancer and anti-inflammatory effects and dexamethasone (DX) has been reported that it also reduces inflammation and inhibits inflammatory…
2019 American Transplant Congress
Examination of a Novel Immunoregulatory Marker in Kidney and Lung Transplant Recipients
A. D. Desai¹, G. J. Kavalam², A. Chandraker², S. Tripathi²
¹1Harvard Summer Research Program for Kidney Medicine, Harvard Medical School,, Boston, MA, ²Schuster Family Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School, Boston, MA
*Purpose: The T-cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain (TIGIT) is a co-inhibitory receptor mainly expressed on T cells and NK cells. TIGIT has…
2019 American Transplant Congress
A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Lymphocyte Biology and Transplant Immunity
L. Qiu¹, J. Wang², R. Fernandez³, X. Yeap¹, S. Han¹, F. Zheng¹, C. Lin², Z. J. Zhang¹
¹Comprehensive Transplant Center, Department of Surgery, Northwestern University, Chicago, IL, ²Northwestern University, Chicago, IL, ³Department of Surgery, Northwestern University, Chicago, IL
*Purpose: Spleen-derived lymphocytes and myeloid cell populations are critical to the development of organ transplant ischemia-reperfusion injury (IRI) and allograft rejection. In this study, we…
2019 American Transplant Congress
One Study: A Consortium Model of Clinical Trials Optimized for Comparing New Immune Therapies via Centralized Immune Monitoring
B. Sawitzki¹, S. Schlickeiser¹, M. Streitz¹, K. Jürchott¹, J. Hutchinson², E. Geissler², P. Investigators³
¹Charite Universitätsmedizin, Berlin, Germany, ²University of Regensburg, Regensburg, Germany, ³ONE Study Consortium, EU, Germany
*Purpose: Immune regulatory cell subpopulations can potentially control immune responses to allow immunosuppression (IS) drug minimization. In the ONE Study, an EU FP7-funded research group…
2019 American Transplant Congress
First Report and Mechanistic Analysis of Chronic GVHD after Solid Organ Transplantation
J. Weiner, N. Svetlicky, J. Schmidt, J. Kang, M. Sadat, A. Karaballa, S. Moturi, O. Aguirre, J. Hawksworth, C. Matsumoto, T. Fishbein, A. Kroemer
Georgetown University Hospital, Washington, DC
*Purpose: Chronic graft-versus-host disease (cGVHD) occurs late after bone marrow transplantation and is associated with a characteristic lichenoid rash (cutaneous sclerosis) and effects on multiple…
2019 American Transplant Congress
Elevated Podocyte-specific Urinary Extracellular Vesicles In Patients With Fsgs Recurrence After Kidney Transplantation
H. Lau, A. Shimomura, E. Tantisattamo, U. G. Reddy, D. Dafoe, H. Ichii
University of California Irvine, Orange, CA
*Purpose: Kidney graft failure is a major concern in patients with kidney transplantation. Patients who suffered graft failure are at a higher risk for mortality…