Articles tagged "FACS analysis"

2021 American Transplant Congress
Adenovirus-Specific T Cells for Steering of Immunosuppression After Pediatric Kidney Transplantation in the Randomized Controlled Ivist Trial
T. Ahlenstiel-Grunow¹, X. Liu², R. Schild³, J. Oh³, C. Taylan⁴, L. T. Weber⁴, H. Staude⁵, M. Verboom⁶, C. Schröder⁷, R. Sabau⁷, A. Großhennig², L. Pape¹
¹Pediatrics II, University Hospital of Essen, Essen, Germany, ²Institute of Biostatistics, Hannover Medical School, Hannover, Germany, ³Pediatric Nephrology, University Hospital Hamburg-Eppendorf, Hamburg, Germany, ⁴Pediatric Nephrology, University of Colgne, Köln, Germany, ⁵Pediatric Nephrology, University Hospital of Rostock, Rostock, Germany, ⁶Institute of Transfusion Medicine and Transplant Engineering, Hannover Medical School, Hannover, Germany, ⁷Institute for Clinical Pharmacology, Hannover Medical School, Hannover, Germany
*Purpose: Pharmacokinetic monitoring alone is insufficient to estimate the intensity of immunosuppression after pediatric kidney transplantation (Tx). Levels of virus-specific T cells (Tvis) have been…
2020 American Transplant Congress
CD56^DimCD16^Bright NK Cells from Kidney Transplant Recipients with Antibody-Mediated Rejection Display Increased Proliferation, Self-Renewal, Pro-Inflammatory and Cytotoxic Profile
E. A. Bailly¹, K. Louis¹, C. Macedo¹, B. Ramaswami¹, M. Lucas¹, A. Zeevi¹, C. Lefaucheur², D. Metes¹
¹Department of Surgery, Thomas E. Starzl Transplantation Institute, Pittsburgh, PA, ²INSERM UMR-S976. Endothelium, Inflammation & Alloreactivity, Université de Paris, France
*Purpose: Contribution of NK cells to antibody-mediated rejection (ABMR) injuries have recently been highlighted through transcriptomic analysis and immunohistochemistry of kidney and heart allograft biopsies.…
2020 American Transplant Congress
Use of Subunit Herpes Zoster Vaccine in VZV-Seronegative Transplant Recipients to Prevent Primary Varicella
A. G. L'Huillier¹, V. H. Ferreira², C. Hirzel³, M. Ierullo², T. Ku², N. Selzner², J. Schiff², J. Tikkanen², C. Miao⁴, D. S. Schmid⁴, A. Humar², D. Kumar²
¹HUG, Geneva, Switzerland, ²UHN, Toronto, ON, Canada, ³Inselspital, Bern, Switzerland, ⁴CDC, Atlanta, GA
*Purpose: Varicella-zoster virus (VZV) primary infection can be prevented by using the live varicella vaccine. However, this is contraindicated after organ transplant. We investigated the…
2020 American Transplant Congress
Evaluation of Pre-Transplant T Cell Exhaustion as a Mechanism for Immune Dysfunction and Recipient Risk of Death Following Liver Transplant
K. E. Lunsford¹, N. Aware², K. Cleveland³, D. T. Nguyen⁴, E. A. Graviss⁴, L. J. Minze³, T. N. Eagar⁴, X. C. Li³, R. M. Ghobrial³
¹Department of Surgery, Rutgers New Jersey Medical School, Newark, NJ, ²Department of Surgery, Houston Methodist Hosptial, Houston, TX, ³Department of Surgery, Houston Methodist Hospital, Houston, TX, ⁴Department of Pathology and Genomic Medicine, Houston Methodist Hospital, Houston, TX
*Purpose: Cirrhosis-related immune dysfunction likely contributes to early mortality following liver transplant (LT) resulting from infections. We previously identified a pre-transplant biomarker panel for immune…
2020 American Transplant Congress
Enumeration of Platelets by Flow Cytometry Reveals Human Erythrocyte Fragmentation During Pig Organ Xenoperfusion Studies
Z. Abady¹, K. M. Petitpas¹, L. Burdorf¹, S. G. Pratts¹, B. M. Cerel¹, S. Sendil², M. R. Connolly¹, W. Qin³, Y. Kan³, J. Layer³, M. Youd³, W. Westlin³, L. Yang³, R. N. Pierson III¹, A. M. Azimzadeh¹
¹Surgery, Massachusetts General Hospital and Harvard School of Medicine, Boston, MA, ²Surgery, University of Maryland School of Medicine, Baltimore, MD, ³eGenesis, Cambridge, MA
*Purpose: Human or baboon platelet sequestration and activation occurs during pig organ xenograft perfusion, although the mechanism remains poorly understood. To evaluate an apparent “rebound”…
2020 American Transplant Congress
Donor-Specific B Cell Frequency Assessed by a Novel Multiplex Bead-Based Assay Predicts Graft Failure in Kidney Transplant Recipients
Z. Ilyas, A. Akl, D. Pinelli, L. Gallon, A. R. Tambur, M. Ansari
Northwestern University Feinberg School of Medicine, Chicago, IL
*Purpose: Kidney transplant recipients with sensitization to HLA defined by a high percentage of Panel Reactive Antibodies (PRA) or Donor-specific anti-HLA antibodies (DSA) are at…
2020 American Transplant Congress
Novel Flow-Based Assay to Detect Allospecific T Cells in Transplantation Study
N. Tanimine¹, B. E. Burrel², J. Pardo², C. Rickert³, K. M. Lee³, K. Deng³, N. Feeney³, C. Leguern³, J. F. Markmann³
¹Department of Gastroenterological and Transplantation Surgery, Hiroshima University, Hiroshima, Japan, ²Immune Tolerance Network, Bethesda, MD, ³Department of Surgery, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA
*Purpose: Identifying and phenotyping donor-reactive T cells in recipients following transplantation may further the understanding of interactions between the recipient’s immune system and donor antigen.…
2020 American Transplant Congress
Examination of the TIGIT/CD226 Axis in Kidney Transplant Recipients
G. J. Kavalam, A. D. Desai, A. Chandraker, S. Tripathi
Renal, Brigham and Women's Hospital, Boston, MA
*Purpose: The T-cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain (TIGIT) and CD226, two poliovirus receptor-like proteins, are an emerging group of immune checkpoints with…
2019 American Transplant Congress
Novel Flow-Based Assay to Identify Allo-Specific T Cell Exhaustion
N. Tanimine¹, C. Rickert¹, K. Lee¹, K. Deng¹, N. Feeney¹, C. Leguern¹, B. Burrell², J. Markmann¹
¹Department of Surgery, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Immune Tolerance Network, Bethesda, MD
*Purpose: A recent, multi-center immunosuppression withdrawal trial in liver transplant recipients unexpectedly revealed time post-transplant as an important predictor for operational tolerance. We hypothesize that…
2019 American Transplant Congress
Extracorporeal Photopheresis for Specific Immune Tolerance Responses in Heart Transplant Recipients
M. J. Barten¹, K. Klaeske², J. Wittke², M. Borger², J. Garbade², S. Lehmann³, H. Reichenspurner¹, M. Dieterlen²
¹Cardiovascular Surgery, University Heart Center, Hamburg, Germany, ²Cardiac Surgery, University Heart Center Leipzig, Leipzig, Germany, ³Cardiacr Surgery, University Heart Center Leipzig, Leipzig, Germany
*Purpose: Extracorporeal photopheresis (ECP) treatment following heart transplantation (HTx) may induce transplant tolerance. A monitoring tool for successful ECP treatment would allow determination of patient-specific…