ATC Abstracts

American Transplant Congress abstracts

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Articles tagged "FACS analysis"

  • 2022 American Transplant Congress

    Characterisation of Kidney-Specific Extracellular Vesicles During Isolated Organ Normothermic Machine Perfusion

    J. Ashcroft1, T. L. Williams2, T. R. Elliott1, A. Marton1, S. A. Hosgood1, M. L. Nicholson1, V. Kosmoliaptsis1

    1Department of Surgery, University of Cambridge, Cambridge, United Kingdom, 2Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

    *Purpose: Introduction: Normothermic machine perfusion (NMP) can evaluate and recondition deceased-donor kidneys but accurate biomarkers are needed to assess organ quality and to predict post-transplant…
  • 2022 American Transplant Congress

    Flow Cytometric Pattern of γδ Tcr Expression Identifies Outcomes in Kidney Transplantation

    S. Sur, S. Pineda, M. Sirota, M. Sarwal

    UCSF, San Francisco, CA

    *Purpose: The main challenge with kidney transplantation is the possibility of rejection, classified into two types, antibody-mediated rejection (ABMR) and T cell-mediated rejection (TCMR). The…
  • 2022 American Transplant Congress

    Protective Immunity Against a Murine Epstein-Barr Virus Homolog is Preserved During CD11b-CD154 Blockade

    K. L. Alexander, D. Liu, M. L. Ford

    Emory University Transplant Center, Emory University, Atlanta, GA

    *Purpose: CD154 pathway antagonism has shown to be a promising target for inducing long-time graft survival, in some cases showing efficacy that is superior to…
  • 2022 American Transplant Congress

    Comprehensive Profiling of Monocytes During Cellular (TCMR) or Mixed Antibody-Mediated Rejection (ABMR) After Kidney Transplantation

    C. Macedo1, E. Bailly1, K. Louis2, X. Gu1, M. Lucas1, A. Zeevi1, P. Randhawa1, A. Bansal3, D. Metes1

    1Starzl Transplantation Institute, Pittsburgh, PA, 2INSERM UMR-S976, Paris, France, 3University of Pittsburgh, Pittsburgh, PA

    *Purpose: Monocytes play an important role in the immune defense by initiating adaptive immunity. They are heterogeneous, plastic, and present different subsets: classical (CD14++CD16-), intermediate…
  • 2022 American Transplant Congress

    Novel Avenue of Allograft Monitoring: Direct Measurement of Donor-Derived Extracellular Vesicles in Human Plasma

    W. W. Woud, D. A. Hesselink, M. J. Hoogduijn, C. C. Baan, K. Boer

    Internal Medicine, Erasmus MC Transplant Institute, Rotterdam, Netherlands

    *Purpose: Extracellular Vesicles (EV) - regarded as “snapshots” of their cell of origin - represent promising liquid biomarkers to monitor allograft function post transplantation. Recently,…
  • 2022 American Transplant Congress

    HLA-Specific IgD/CD27 Double Negative B Cell Frequency Assessed by a Novel Multiplex Bead-Based Assay Predicts Rejection in Kidney Transplant Recipients

    Z. Ilyas1, A. Akl2, D. Pinelli1, L. Gallon3, A. Tambur1, M. Ansari4

    1Northwestern University, Chicago, IL, 2Dr. Soliman Fakeeh Hospital, Jeddah, Saudi Arabia, 3Northwestern Univ NMFF, Chicago, IL, 4Medicine, Northwestern University, Chicago, IL

    *Purpose: Recipients with sensitization to HLA defined by a high percentage of Panel Reactive Antibodies (PRA) or Donor-specific anti-HLA antibodies (DSA) are at higher immunologic…
  • 2022 American Transplant Congress

    Combined CyTOF and Elispot Investigation of CMV Response Over the First Year Post Kidney Transplantation

    T. Jouve1, L. Donadeu Casassas2, S. Hartzell3, E. Crespo2, M. Meneghini2, A. Torija2, P. Cravedi4, O. Bestard2

    1Nephrology, Vall d’Hebron Institut de Recerca (VHIR), Grenoble, Spain, 2Nephrology, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain, 3Mount Sinai Hospital, New York, NY, 4Mount Sinai, New York, NY

    *Purpose: CMV remains a threat for kidney transplant recipients (KTR). Deciphering the impact of CMV on the immune system of KTR has helped improving CMV…
  • 2022 American Transplant Congress

    Donor-Specific Hyporesponsiveness Following Kidney Transplantation is Explained by Progressive Loss of Donor-Reactive Polyfunctional Cd4+ Effector Memory T Cells

    A. C. van der List, N. H. Litjens, M. Klepper, F. Prevoo, M. G. Betjes

    Internal Medicine, Erasmus MC, Rotterdam, Netherlands

    *Purpose: After kidney transplantation, donor-specific hyporesponsiveness (DSH) develops which is defined as a lowered response of alloreactive T cells to donor antigen while retaining response…
  • 2022 American Transplant Congress

    Deletion of Fibroblastic Reticular Cell Derived Laminin A4 Impairs Lymph Node Vasculature and Conduits

    L. Li1, M. Shirkey1, W. Piao1, A. Kensiski1, V. Saxena1, T. Zhang1, X. Li2, J. Zhao2, S. Gavzy1, R. Abdi2, J. Bromberg1

    1UMB, Baltimore, MD, 2Harvard University, Boston, MA

    *Purpose: Fibroblastic reticular cells (FRCs) support lymph node (LN) structure by producing various functional extracellular matrix (ECM) components. We hypothesized that FRCs and their ECM…
  • 2022 American Transplant Congress

    Longitudinal Analysis of the Gut Microbiota Structure and Function After Renal Transplantation

    A. Vaitkute1, F. Yuen Chang1, A. Greco2, S. Chong1, H. Mahdi3, M. Bajaj-Elliott2, A. Pesenacker1, R. Motallebzadeh1

    1Institute of Immunity and Transplantation, UCL, London, United Kingdom, 2Institute of Child Health, UCL, London, United Kingdom, 3Department of Renal Medicine, UCL, London, United Kingdom

    *Purpose: Despite marked improvements in outcomes after renal transplantation, graft loss from alloimmune pathology and adverse side effects including urinary tract infections and diarrhoea remain…
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