Articles tagged "Epitopes"

2018 American Transplant Congress
The Brazilian Epitope Virtual Crossmatch Protocol: A Reliable Tool for Increasing Kidney Allocation among Highly Sensitized Patients
L. Sousa,¹ A. Silva,¹ M. Marroquim,¹ A. Coelho,¹ A. Lima,¹ F. Silva,¹ C. Carracosa,² G. Willcox,³ T. Leão,³ B. Correa,³ E. Guimarães,³ G. Maciel,³ J. Andrade,⁴ A. Andrade,⁴ R. Neto,⁴ S. Cavalcante,⁴ C. Leão,⁴ F. Cavalcante,⁵ A. Holanda,⁵ N. Gomes,⁶ A. Rego,⁶ S. Monte.¹
¹UFPI, Teresina, PI, Brazil; ²PUC, Curitiba, PR, Brazil; ³HLA Diagnostico, Recife, PE, Brazil; ⁴IMIP, Recife, PE, Brazil; ⁵RHP, Recife, PE, Brazil; ⁶CNCDO, Recife, PE, Brazil.
Background: In Brazil (BZ), the kidney allocation policy for highly sensitized patients (HS) (PRA ≥ 80%) does not involve prioritization and transplants 10 times less…
2017 American Transplant Congress
Kidney Transplant Rejection Associated with DQ Eplet Mismatches.
S. Chakola,¹ P. Warner,² N. Andeen,³ B. Najafian,³ N. Leca,¹ C. Blosser.¹
¹Medicine, University of Washington, Seattle, WA; ²Bloodworks Northwest, Seattle, WA; ³Pathology, University of Washington, Seattle, WA
The presence of anti-HLA antibodies is associated with acute rejection, allograft dysfunction and earlier graft loss. HLA antibodies are recognized as being specific to these…
2017 American Transplant Congress
Precise Antibody Characterization Using Multiple Solid Phase Assay Platforms Is Critical for Accurate Virtual Crossmatching.
D. Li,¹ O. Timofeeva,¹ M. Awwad,¹ C. Cody,² S. Najjar,² S. Rosen-Bronson.¹
¹Histocompatibility Laboratory, MedStar Georgetown University Hospital, Washington, DC; ²MedStar Heart Institute, Washington Hospital Center, Washington, DC
Purpose: Virtual crossmatch (VXM) has been utilized in heart transplant (txp) for more than a decade. Many labs base the VXM solely on single antigen…
2017 American Transplant Congress
Shared Epitopes Contribute to Accumulation of Highly Sensitized Patients in Kidney Paired Donation Program.
S. Stepkowski, B. Mierzejewska, C. Baum, D. Bekbolsynov, R. Brunner, J. Kopke, S. Rees, M. Rees.
University of Toledo, Toledo, OH
Introduction: Kidney Paired Donation (KPD) programs suffer from the accumulation of highly sensitized patients. We evaluated the sensitization patterns in our KPD program-by comparison of…
2017 American Transplant Congress
HLA Eplet Mismatches Predict DSA Formation, Graft Survival, and Re-Transplantation Rate in Pediatric Renal Transplantation.
M. Sypek,^1,2 S. Hiho,³ L. Cantwell,³ P. Hughes,² A. Le Page,⁴ J. Kausman.¹
¹Royal Children's Hospital, Melbourne, Australia; ²Royal Melbourne Hospital, Melbourne, Australia; ³Victorian Transplant and Immunogenetics Service, Melbourne, Australia; ⁴Monash Children's Hospital, Melbourne, Australia
The potential benefits of HLA epitope based matching are greatest for pediatric recipients, however, there is little published data on the association of epitope mismatches…
2017 American Transplant Congress
Donor Recipient Matching Based on HLA Epitopes Improves Outcome in Kidney Transplant Recipients.
O. Staeck,¹ M. Niemann,³ F. Halleck,¹ D. Khadzhynov,¹ C. Schönemann,² P. Reinke,¹ E. Spierings,⁴ K. Budde,¹ N. Lachmann.²
¹Nephrology, Charité Universitaetsmedizin, Berlin, Germany; ²HLA Laboratory, Charité Universitaetsmedizin, Berlin, Germany; ³PIRCHE, Berlin, Germany; ⁴Laboratory of Translational Immunology, UMC Utrecht, Utrecht, Netherlands
Background: HLA mismatches of the donor and recipient causing de novo donor-specific HLA antibodies (dnDSA) are a risk factor for immunologic complications and poor graft…
2016 American Transplant Congress
Should HLA DR and DQ Eplet Mismatching Replace Traditional DR and DQ Mismatching for Deceased Donor Offers in Pediatric Renal Transplantation?
C. Bryan, V. Chadha, B. Warady.
Midwest Transplant Network, Kansas City, KS; Nephrology, Childrens Mercy Hospital, Kansas City, MO; Nephrology, Childrens Mercy Hospital, Kansas City, MO.
Introduction. It is now appreciated that greater HLA DR mismatching of the first pediatric kidney transplantation is associated with higher degrees of sensitization, lower rates…
2016 American Transplant Congress
The Distance Between Us: The Landscape of Recipient-Specific Loss-of-Function in Solid Organ Transplantation and Association with Rejection-Free Graft Survival.
B. Cole,^1,2,5 J. van Setten,^3,5 B. Keating.^1,4,5
¹Department of Surgery, The Penn Transplant Institute, Philadelphia, PA; ²Center for Clinical Epidemiology and Biostatistics, The Institute for Biomedical Informatics, Philadelphia, PA; ³Department of Medical Genetics, University Medical Center Utrecht, Utrecht, Netherlands; ⁴Department of Surgery, Division of Transplant, the Children's Hospital of Philadelphia, Philadelphia, PA; ⁵The Omics Working Group, iGeneTRAiN Consortium, Philadelphia, PA.
Purpose: This study aims to comprehensively identify genes that are inactivated in both copies in transplant recipients but not in their respective donors, uncovering a…
2015 American Transplant Congress
Differences in Reactivity of HLA-Specific Antibodies May Be Explained By Differences in Their Affinities for Selected Epitopes on HLA Proteins
S. Daga,^1,2 D. Lowe,⁶ R. Buchli,⁵ J. Collard,⁵ A. Mulder,⁸ C. McMurtrey,^4,5 H. Moyse,⁷ N. Evans,⁷ N. Krishnan,² W. Hildebrand,^4,5 F. Claas,⁸ D. Briggs,³ D. Zehnder,^1,2 D. Mitchell,¹ R. Higgins.^1,2
¹Clinical Sciences Research Laboratories, University of Warwick, Coventry, United Kingdom; ²Renal Unit, University Hospital, Coventry and Warwickshire NHS Trust, Coventry, United Kingdom; ³Department of Histocompatibility and Immunogenetics, NHS Blood and Transplant, Birmingham, United Kingdom; ⁴University of Oklahoma Health Science Center, Oklahoma City; ⁵Pure Protein LLC, Oklahoma City; ⁶Department of Histocompatibility and Immunogenetics, Royal Liverpool University Hospital, Liverpool, United Kingdom; ⁷School of Engineering, University of Warwick, Coventry, United Kingdom; ⁸Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, Netherlands.
Affinity of IgG immunoglobulins for antigen varies and matures within the evolution of immune responses. Affinity is likely to be different for different epitope-antibody interactions…
2015 American Transplant Congress
Comparative Survival Benefits and Costs of an Eplet and Broad Antigen Based Matching System in Deceased Donor Kidney Transplant Allocation Among Indigenous Australians
H. Do Nguyen,¹ G. Wong,^2,3 J. Chapman,² J. Craig,^2,3 L. D'Orsogna,⁴ W. Lim.^1,5
¹School of Medicine and Pharmacology, University of Western Australia, Crawley, Western Australia, Australia; ²Nephrology, The Children's Hospital at Westmead, Sydney, New South Wales, Australia; ³School of Medicine, University of New South Wales, Sydney, New South Wales, Australia; ⁴Clinical Immunology, Royal Perth Hospital, Perth, Western Australia, Australia; ⁵Renal Medicine, Sir Charles Gairdner Hospital, Nedlands, Western Australia, Australia.
Aim: To compare the benefits and costs of incorporating an eplet-based matching algorithm to the current allocation of deceased donor kidneys to indigenous Australians.Background: The…