Articles tagged "Epitopes"

2019 American Transplant Congress
Targeted Epitope Mutagenesis as a Strategy to Decrease Xenoantibody Binding
J. Ladowski, G. Martens, Z. Wang, L. Reyes, M. Tector, J. Tector
University of Alabama-Birmingham, Birmingham, AL
*Purpose: Xenotransplantation, the use of genetically-modified pig organs for transplantation, has been limited by antibody-mediated rejection. The generation of a three-antigen knockout pig provided a…
2019 American Transplant Congress
Mismatched Epitope and De Novo DSA Development: An Analysis of the Benefit and Benefit-EXT Renal Transplant Cohorts
V. Jucaud¹, V. Burnett¹, H. Gebel², R. Bray², M. Polinsky³, M. Everly¹
¹Terasaki Research Institute, Los Angeles, CA, ²Emory University, Atlanta, GA, ³Bristol-Myers Squibb, Princeton, NJ
*Purpose: De novo donor-specific HLA antibodies (dnDSA) target epitopes rather than whole antigens, and their development is associated with the mismatched HLA antibody epitope load…
2019 American Transplant Congress
Solving Target Recognition and Activation Mediated by Non-HLA Agonistic Antibodies Targeting ETAR
A. Philippe¹, R. Catar¹, P. Scheerer², M. Szczepek², D. Dragun¹
¹Nephrology and Medical Intensive Care, Charité Universitätsmedizin Berlin, Berlin, Germany, ²Institute of Medical Physics and Biophysics, Charité Universitätsmedizin Berlin, Berlin, Germany
*Purpose: Endothelin-1 type A receptor (ETAR) exhibits multiligand binding abilities and may signal upon natural ligand Endothelin-1 (ET-1) and non-HLA ETAR-agonistic IgG (ETAR-IgG)-mediated stimulation similar…
2018 American Transplant Congress
Eplet Mismatches and De Novo Donor Surface Antibody Development in Pediatric Renal Transplant Recipients
E. Blanchette,¹ M. Bradford,¹ P. Warner,² C. Blosser,³ I. Gimferrer,² J. Smith.¹
¹Seattle Children's Hospital, Seattle; ²Bloodworks Northwest, Seattle; ³UW Medicine, Seattle.
Background: De novo donor surface antibody (dnDSA) development is arguably the first step in the natural history of allograft injury secondary to humoral alloimmunity. This…
2018 American Transplant Congress
Epitope/Eplet Mismatch in Pediatric Renal Transplantation and Development of Donor Specific Antibody
R. George,¹ H. Sullivan,² R. Garro,¹ P. Winterberg,¹ B. Warshaw,¹ H. Gebel,² R. Bray.²
¹Pediatric Nephrology, Emory University & Children's Healthcare of Atlanta, Atlanta, GA; ²Dept. of Pathology, Emory Transplant Center, Emory University, Atlanta, GA.
Background: De-novo donor specific antibody (DSA) is one of the strongest independent predictors of graft loss with HLA mismatching being a major risk factor for…
2018 American Transplant Congress
Antibody-Verified Eplet Mismatches as Determinants of Premature Kidney Transplant Loss
R. Sapir-Pichhadze,¹ X. Zhang,¹ A. Ferradji,¹ A. Madbouly,² S. Fingerson,² K. Tinckam,⁵ H. Gebel,³ H. Cardinal,⁴ B. Foster.¹
¹McGill University, Montreal, Canada; ²National Marrow Donor Program, Minneapolis; ³Emory University Hospital, Atlanta; ⁴CHUM, Montreal, Canada; ⁵University Health Network, Toronto, Canada.
Introduction: HLA matching at the epitope level offers new opportunities to identify compatible donors for transplant candidates. HLAMatchmaker deduces B-cell epitope ('eplets' in HLAMatchmaker) mismatches…
2018 American Transplant Congress
HLA Eplet Mismatch and Donor Specific Antibodies in Kidney Transplantation
S. Wan,^1,2 S. Angel de Wilde,² B. Rosales,² S. Chadban,^1,2 K. Wyburn.^1,2
¹Department of Renal Medicine, Royal Prince Alfred Hospital, Sydney, Australia; ²Sydney Medical School, University of Sydney, Sydney, Australia.
BACKGROUND: Although HLA matching improves graft survival in kidney transplant recipients (KTx), eplet mismatches provide higher resolution information, and could better predict the development of…
2018 American Transplant Congress
Can We Expect the “Unexpected”?
D. Pinelli, A. Tambur.
Northwestern University, Chicago.
A known, seemingly paradoxical, phenomenon of the HLA system is the high overall degree of homology among different HLA alleles, despite vast polymorphism in the…
2018 American Transplant Congress
The Brazilian Epitope Virtual Crossmatch Protocol: A Reliable Tool for Increasing Kidney Allocation among Highly Sensitized Patients
L. Sousa,¹ A. Silva,¹ M. Marroquim,¹ A. Coelho,¹ A. Lima,¹ F. Silva,¹ C. Carracosa,² G. Willcox,³ T. Leão,³ B. Correa,³ E. Guimarães,³ G. Maciel,³ J. Andrade,⁴ A. Andrade,⁴ R. Neto,⁴ S. Cavalcante,⁴ C. Leão,⁴ F. Cavalcante,⁵ A. Holanda,⁵ N. Gomes,⁶ A. Rego,⁶ S. Monte.¹
¹UFPI, Teresina, PI, Brazil; ²PUC, Curitiba, PR, Brazil; ³HLA Diagnostico, Recife, PE, Brazil; ⁴IMIP, Recife, PE, Brazil; ⁵RHP, Recife, PE, Brazil; ⁶CNCDO, Recife, PE, Brazil.
Background: In Brazil (BZ), the kidney allocation policy for highly sensitized patients (HS) (PRA ≥ 80%) does not involve prioritization and transplants 10 times less…
2018 American Transplant Congress
Correlation of Kidney Transplant Human Leukocyte Antigen Eplet Mismatching to De Novo Donor Specific Antibody Production and Post-Transplant Outcomes
R. Samudralwar,¹ K. Szempruch,¹ C. Reuter,³ B. Franz,⁴ R. Lee,¹ E. Weimer,⁴ R. Detwiler,² T. Kozlowski,² J. Schmitz.⁴
¹Department of Pharmacy, Universitty of North Carolina Medical Center, Chapel Hill, NC; ²Department of Surgery, Universitty of North Carolina Medical Center, Chapel Hill, NC; ³Department of Pharmacy, Ochsner Health System, New Orleans, LA; ⁴Department of Pathology, University of North Carolina Medical Center, Chapel Hill, NC.
Human Leukocyte Antigen (HLA) eplet mismatches (EMM) are associated with the development of de novo donor specific antibody (dnDSA) in kidney transplant recipients (KTRs). There…