Articles tagged "Bioengineering"

2018 American Transplant Congress
Re-Endothelialization of Liver Xenografts Utilizing Human Endothelial Cells
W. Hassanein,¹ P. Patel,¹ A. Werdsheim,¹ J. Brilliant,¹ E. Buckingham,¹ N. Shockcor,¹ C. Drachenberg,² R. Barth,¹ J. LaMattina.¹
¹Surgery, University of Maryland, Baltimore, MD; ²Pathology, University of Maryland, Baltimore, MD.
Introduction:Acute antibody mediated rejection due to preformed antibodies present in human serum remains a hurdle to successful xenotransplantation. Xenograft vascular endothelium represents the initial site…
2018 American Transplant Congress
Successful Patency in Blood Conduits for an Artificial Kidney Membrane with Biocompatible Coatings
R. Forbes,¹ E. Marsh,¹ S. Karp,¹ A. Buck,¹ D. Colvin,¹ P. Williams,¹ B. Chaudhuri,² S. Roy,² W. Fissell.¹
¹VUMC, Nashville; ²UCSF, San Francisco.
BACKGROUND: An implantable artificial kidney may address the growing organ shortage and improve outcomes and quality of life for those with end stage renal disease.…
2018 American Transplant Congress
Liver Preservation with Machine Perfusion and a New Human-Derived Hemoglobin-Based Oxygen Carrier Solution (HDHBOC)
W. R. Light,¹ A. Malavalli,¹ K. Vandegriff,¹ P. Fontes.²
¹VirTech Bio, Inc, Natick, MA; ²University of Pittsburgh, Pittsburgh, PA.
Introduction: Machine perfusion (MP) is becoming the new standard for liver preservation following the successful implementation of devices for kidney and lung preservation ex-vivo. Liver…
2018 American Transplant Congress
Activity Nanosensors for Early and Noninvasive Detection of Acute Organ Transplant Rejection
Q. Mac,¹ D. Mathews,² J. Kahla,¹ C. Stoffers,¹ O. Delmas,¹ B. Holt,¹ A. Adams,² G. Kwong.¹
¹Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA; ²Department of Surgery, Emory University School of Medicine, Atlanta, GA.
Purpose: We engineered activity nanosensors that sense anti-allograft T cell activity in vivo to noninvasively detect the onset of acute cellular rejection from urine.Methods: Granzyme…
2018 American Transplant Congress
Engineering Regulatory T Cells with Interleukin (IL-)2 Nanogel for Improved Alloimmune Suppression
S. Eskandari,¹ M. Bandeira Melo,² J. Assaker,¹ A. Mansouri,¹ S. Cai,¹ B. Al Dulaijan,¹ M. Mohamed,¹ D. Irvine,² J. Azzi.¹
¹Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School, Boston, MA; ²Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA.
Clinical trials of adoptively transferred regulatory T cells (TRegs) have been hindered by the short lifespan of these transferred TRegs. Systemic injection of low-dose interleukin…
2017 American Transplant Congress
New Approach for Targeted Immunotherapy in Vascularized Composite Allotransplantation.
F. Feturi,¹ H. Wang,² Y. Brudno,² V. Erbas,³ Z. Zhang,³ H. Sahin,³ D. Mooney,² V. Gorantla,³ R. Venkataramanan.¹
¹Department of Pharmaceutical Science, University of Pittsburgh School of Pharmacy, Pittsburgh, PA; ²A Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA; ³Department of Plastic and Reconstructive Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA
Vascularized composite allotransplantation (VCA) have highly immunogenic skin component mandates high doses of systemic immunosuppressive drugs. Oral dosing leads to fluctuating, unpredictable blood levels that…
2017 American Transplant Congress
Tofacitinib-Loaded Solid Lipid Nanoparticles Inhibits Dendritic Cell Antigen Presenting Functions and Achieve Selective In Vivo Targeting.
G. Raimondi,¹ X. Calderon-Colon,² J. Budihardjo,¹ A. Mirdad,¹ M. Iglesias-Lozano,¹ J. Walch,¹ W. Lee,¹ G. Brandacher,¹ J. Patrone.²
¹Plastic & Reconstructive Surgery, Johns Hopkins University, Baltimore; ²Johns Hopkins Applied Physics Laboratory, Laurel
BackgroundComplications from long-term use of calcineurin inhibitors are a major concern for transplant patients. To develop CNI-free therapies, we investigated an innovative drug delivery method…
2017 American Transplant Congress
Measurement of Dissolved Oxygen by ¹⁹F MRI of Microcapsules Implanted IP in Non-Human Primates.
S. Safley,¹ M. Graham,² M. Garwood,³ K. Papas,⁵ B. Weegman,^3,4 G. Barber,¹ E. Lin,¹ J. Leisen,⁷ A. Sambanis,⁶ B. Hering,² C. Weber.¹
¹Surgery, Emory University, Atlanta, GA; ²Surgery, University of Minnesota, Minneapolis, MN; ³Radiology, University of Minnesota, Minneapolis, MN; ⁴Sylvatica Biotech, Inc., Charleston, SC; ⁵Surgery, University of Arizona, Tucson, AZ; ⁶School of Chemical and Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA; ⁷School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA
Since our encapsulated NHP islet allografts and porcine islet xenografts fail after i.p. transplantation in immunosuppressed NHPs without a cellular or humoral immune response, we…
2017 American Transplant Congress
Binding of Human Neutrophils to Activated Porcine Endothelium Is Mediated by E-Selectin.
C. Laird,¹ B. French,¹ L. Burdorf,¹ X. Cheng,¹ W. Fogler,² J. Magnani,² C. Phelps,³ D. Ayares,³ A. Azimzadeh,¹ R. Pierson.¹
¹University of Maryland SOM, Baltimore; ²GlycoMimetics, Inc, Rockville; ³Revivicor, Inc, Blacksburg
Purpose: Shortage of transplantable human organs motivates research into alternatives, including xenotransplantation. When porcine lungs are perfused with human blood, a majority of human neutrophils…
2017 American Transplant Congress
Decellularized Rat Liver Scaffolds Support Survival and Metabolic Function of Multilineage Allogenic Cells.
W. Hassanein, A. Werdesheim, A. Cimeno, B. Buckingham, A. Khalifeh, M. Uluer, J. Harrison, D. Parsell, C. Drachenberg, R. Barth, J. LaMattina.
University of Maryland School of Medcine, Baltimore, MD
Background:Organ bioengineering has the potential to completely eliminate the donor organ shortage. Here, we examine the feasibility of a decellularized liver construct to support the…