Articles tagged "Bioengineering"

2022 American Transplant Congress
Addressing the Organ Shortage, Improving Transplant Longevity, and Enabling Minimally Invasive Kidney Transplantation: Development of an Intraoperative Kidney Anastomosis Facilitation and Regelation Device
K. S. Hansen¹, R. Meier², J. Gardner¹
¹University of California San Francisco, San Francisco, CA, ²Division of Transplant Surgery, University of Maryland, San Francisco, CA
*Purpose: Second warm ischemia time (SWIT) of a renal allograft during anastomosis increases rates of delayed graft function and premature graft failure. In the setting…
2022 American Transplant Congress
Engineered IL-2 Plus Calcineurin Inhibitor Synergistically Expands Regulatory T Cells Transduced with Engineered IL-2 Receptor and Facilitates Establishment of Transplantation Tolerance
T. Hirai¹, P. Lin², T. L Ramos², K. Garcia³, R. S Negrin²
¹Urology, Tokyo Women's Medical University, Tokyo, Japan, ²Blood and Marrow Transplantation, Stanford University, Stanford, CA, ³Molecular and Cellular Physiology and Structural Biology, Stanford University, Stanford, CA
*Purpose: Clinical trials for regulatory T cell (Treg) therapy in organ transplant has shown promising results. However, it is still under the debate which drugs…
2022 American Transplant Congress
Immunometabolism of Regulatory T Cells Enhanced by Lipid Nanoparticles
J. S. Rink¹, Y. Sambandam², J. He², J. Mathew³, J. R. Leventhal⁴, C. S. Thaxton⁵
¹Department of Medicine, Division of Hematology/ Oncology, Northwestern University, Chicago, IL, ²Comprehensive Transplant Center, Northwestern University, Chicago, IL, ³Northwestern University, Chicago, IL, ⁴Surgery, Northwestern Medicine, Chicago, IL, ⁵Department of Urology, Northwestern University, Chicago, IL
*Purpose: Human regulatory T cell (Treg) metabolism is highly dependent upon a number of factors, including cholesterol obtained from circulating lipoproteins. We have developed high-density…
2022 American Transplant Congress
Vitrification of Pancreatic Islets and Transplantation
J. S. Rao¹, L. Zhan¹, D. Tobolt¹, M. Q. Slama², N. Sethia¹, Z. Han¹, C. S. Dutcher¹, Q. Peterson², J. C. Bischof¹, E. B. Finger¹
¹University of Minnesota, Minneapolis, MN, ²Mayo Clinic, Rochester, MN
*Purpose: A limitation to potential cure for diabetes through islet transplantation is insufficient islet equivalence (IEQ) from a single donor to achieve insulin independence in…
2022 American Transplant Congress
Tissue Engineering Scaffolds for Minimally Invasive Immunosurveillance of Acute Cellular Graft Rejection
R. R. Urie¹, D. Farris², A. Morris¹, E. Lombard¹, J. Z. Li³, D. Goldstein², L. Shea¹
¹Biomedical Engineering, University of Michigan, Ann Arbor, MI, ²Internal Medicine, University of Michigan, Ann Arbor, MI, ³Human Genetics, University of Michigan, Ann Arbor, MI
*Purpose: As there is no assay to predict the risk of acute cellular allograft rejection (ACAR), clinicians rely on protocol graft biopsy and aggressive, one-size-fits-all…
2022 American Transplant Congress
Voxelated Bioprinting of Mechanically Robust Multiscale Porous Scaffolds for Islet Transplantation
J. Zhu, Y. He, Y. Wang, J. Oberholzer, L. Cai
University of Virginia, Charlottesvle, VA
*Purpose: Classical microencapsulation technologies for islet transplantation faces two major challenges associated with individual microcapsules. First, due to long-term gravity individual microcapsules often aggregate to…
2022 American Transplant Congress
Functional Recellularized Patient Derived Endothelium; A Human Vascular Graft Approach
H. Tejeda Mora¹, J. Willemse², Y. den Hartog¹, I. Schurink², M. M. Verstegen², J. de Jonge², R. C. Minnee², M. W. van den Hoogen², C. C. Baan¹, L. J. van der Laan², M. J. Hoogduijn¹
¹Transplant Institute - Internal Medicine, Erasmus Medical Center, Rotterdam, Netherlands, ²Transplant Institute - Department of Surgery, Erasmus Medical Center, Rotterdam, Netherlands
*Purpose: In transplantation, the endothelial lining is the first barrier between the donor organ and recipient immune system. Damaged endothelium exposes extracellular matrix (ECM) molecules…
2021 American Transplant Congress
Islet Allotransplantation Into Pre-vascularized Sernova Cell Pouch^TM – Preliminary Results from the University of Chicago
P. J. Bachul¹, P. E. Borek¹, G. S. Generette¹, A. Perez-Gutierrez¹, K. Jayant¹, K. Golab¹, L. Basto¹, L. Perea¹, M. Tibudan¹, C. Thomas², L. Philipson², J. Fung¹, P. Witkowski¹
¹Surgery, University of Chicago, Chicago, IL, ²Medicine, University of Chicago, Chicago, IL
*Purpose: Previous first-in-human pilot study showed safety but the conditions did not allow for detectable islet function post-transplantation into the pre-vascularized Sernova Cell Pouches (SCPs)…
2021 American Transplant Congress
Cross-Circulation for Extracorporeal Liver Support in a Swine Model
W. K. Wu¹, A. Tumen¹, J. W. Stokes², R. Ukita², A. E. Hozain³, C. R. Flynn², M. J. Lee³, J. R. Talackine², N. L. Cardwell², J. A. Reimer³, M. Pinezich³, C. Benson², G. Vunjak-Novakovic³, S. P. Alexopoulos², M. Bacchetta²
¹co-first authors, Vanderbilt University Medical Center, Nashville, TN, ²Vanderbilt University Medical Center, Nashville, TN, ³Columbia University Medical Center, New York, NY
*Purpose: Although machine perfusion has gained momentum as an organ preservation strategy in liver transplantation, significant organ shortages and waitlist mortality persist, highlighting unmet needs…
2021 American Transplant Congress
Enhancement of Regulatory T Cell Therapies by Biomimetic Nanofiber Matrices on Single Cells
L. A. Fraser¹, S. Lee¹, Y. Sambandam², J. He², X. Huang², D. Z. Ivancic², J. Mathew², Z. J. Zhang², J. R. Leventhal², L. Gallon², S. I. Stupp¹
¹Simpson Querrey Institute, Northwestern, Chicago, IL, ²Surgery – Comprehensive Transplant Center, Northwestern, Chicago, IL
*Purpose: Here we report a new method to create an artificial and biodegradable matrix of biomimetic nanofibers on single cells with the goal of enhancing…