Articles tagged "Allorecognition"

2021 American Transplant Congress
K562 Cells Expressing HLA-A2 Stimulate Alloreactive CD8+ T Cells
A. B. Morris, E. Peek, A. Hadley, C. P. Larsen
Emory Transplant Center, Emory University, Atlanta, GA
*Purpose: T cell-mediated rejection is a significant factor that leads to graft damage and loss in transplant recipients. Due to the complexity of the TCR:pMHC…
2021 American Transplant Congress
Precision Medicine in Renal Transplantation: T-cell Repertoire Sequencing to Define Donor-specific Clones for Post-transplant Monitoring
K. R. Sherwood¹, P. Wong¹, F. Fenninger¹, V. Wu¹, J. Beckrud², D. P. Cina³, L. Allan², J. Lan², P. A. Keown²
¹University of British Columbia, Vancouver, BC, Canada, ²Vancouver General Hospital, Vancouver, BC, Canada, ³Urologic Sciences, Vancouver General Hospital, Vancouver, BC, Canada
*Purpose: Measuring donor-specific clonal responses offers a precise, sensitive and specific biomarker of immune recognition and activation. Combining deep immunophenotyping (DI) and T-cell receptor (TCR)…
2020 American Transplant Congress
Offspring to Parent Living Donor Liver Transplantation: An Analysis of the A2ALL Database
R. A. Choudhury¹, H. J. Braun², J. D. Grab², J. L. Dodge², H. B. Moore¹, D. Yoeli¹, J. J. Pomposelli¹, T. Nydam¹, N. L. Ascher², E. A. Pomfret¹, J. P. Roberts²
¹Department of Surgery- Division of Transplantation Surgery, University of Colorado Hospital, Aurora, CO, ²Department of Surgery- Division of Transplantation Surgery, University of California, San Francisco, San Francisco, CA
*Purpose: Recently, there have been concerns regarding inferior graft survival following offspring to parent kidney transplantation due to issues related to alloimmunity during pregnancy. It…
2020 American Transplant Congress
Determination of Programmed Cell Death Protein 1 (PD1) by Flow Cytometry, a Possible Follow-Up Tool in Renal Transplantation of High Immune Risk
M. Carmona-Escamilla¹, M. Fonseca², J. Prieto Chávez³, L. Arriaga⁴, F. Oviendo⁵, S. , Hernández-Mendoza SA², J. Alberu⁶, G. Queipo²
¹Nephrology, Hospital Central Sur Alta Especialidad "Picacho", CDMX, Mexico, ²Genetics, Hospital General de México Dr. Eduardo Liceaga-Facultad, Ciudad de México, México, CDMX, Mexico, ³Transplant, Hospital General de México Dr. Eduardo Liceaga-Facultad, Ciudad de México, México, CDMX, Mexico, ⁴Unidad de investigación en inmunoqímica,, Hospital de Especialidades Centro Médico Siglo XXI IMSS, CDMX, Mexico, ⁵Unidad de investigación en inmunoqímica,, Hospital de Especialidades Centro Médico Siglo XXI IMSS, Ciudad de México, México, CDMX, Mexico, ⁶Transplant, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, CDMX, Mexico
*Purpose: Introduction: Currently, the follow-up of patients remains to be the serum creatinine level, being highly specific, however, with little sensitivity to determine the occurrence…
2020 American Transplant Congress
Role of Innate Allorecognition Pathways in Mouse Kidney and Heart Allograft Rejection
D. Zhao¹, H. Dai¹, K. Abou-Daya¹, R. Tieu¹, A. Williams¹, M. Oberbarnscheidt¹, X. Li², F. Lakkis¹
¹Thomas E. Starzl Transplantation Institute, Pittsburgh, PA, ²Houston Methodist Hospital Immunobiology & Transplant Science Center, Houston, TX
*Purpose: Paired immunoglobulin-like receptors (PIR) and CD47 are molecules expressed on host myeloid cells. They recognize donor polymorphic MHC and signal regulatory protein alpha (SIRPα),…
2020 American Transplant Congress
Flow Cytometric Characterization of Regulatory Cell Populations Expanded Ex Vivo from PBMCs of Kidney Transplant Recipients
S. Tripathi¹, P. L. Martin-Moreno², G. J. Kavalam¹, A. Chandraker¹
¹Renal, Brigham and Women's Hospital, Boston, MA, ²Nephrology, Clinica Universidad de Navarra, Instituto de Investigación Sanitaria de Navarra (IdiSNA), Pamplona, Spain
*Purpose: We have previously shown that it is possible to generate alloantigen-specific regulatory T cell lines and clones that prolong allograft survival upon infusion in…
2020 American Transplant Congress
T Cell Activation by Allogeneic EVs and Allogeneic MHC Cross-Dressed Cells In Vitro and In Vivo
A. Prunevieille¹, B. Gonzalez-Nolasco¹, G. Benichou¹, N. Mooney², A. Colleen³
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²INSERM, Paris, France, ³Boston College, Boston, MA
*Purpose: The recognition of donor-MHC molecules by recipient T cells triggers the immune response leading to rejection of allografts. Our recent studies have documented the…
2020 American Transplant Congress
Costimulatory Signal Impacts Car Treg Fate and Function in Transplantation
B. Lamarthée¹, A. Marchal¹, S. Charbonnier¹, T. Blein¹, K. Vogt², M. Titeux¹, N. Pallet³, M. Delville¹, E. Six¹, D. Anglicheau⁴, C. Legendre⁴, B. Sawitzki², J. Taupin⁵, M. Cavazzana¹, I. André¹, J. Zuber¹
¹Inserm U1163, Institut Imagine, Paris, France, ²Department of Immunology, Charité University Hospital, Berlin, Germany, ³Inserm U1147, Centre Universitaire des Saints Pères, Paris, France, ⁴Service de Transplantation Rénale Adulte, Assistance Publique–Hôpitaux de Paris, Hôpital Necker, Paris, France, ⁵Inserm U1160, Centre Hayem, Paris, France
*Purpose: Donor-specific regulatory T-cell (Treg) therapy has emerged as a potent strategy to promote immune tolerance in experimental transplantation. However, a major stumbling block in…
2020 American Transplant Congress
Belatacept Suppresses the B-Cell Alloresponse in the Lower Range of Therapeutic Trough Concentrations
C. Ashokkumar¹, R. Townsend², M. Roberts², T. Lehman², B. Gavin², B. Spishock¹, R. Sindhi¹
¹Plexision, Pittsburgh, PA, ²Bristol-Myers-Squibb, Princeton, NJ
*Purpose: Background: Kidney recipients treated with the T-cell costimulation blocker, belatacept, experience a higher incidence of acute cellular rejection (ACR) and lower incidence of donor-specific…
2020 American Transplant Congress
Infectious Tolerance Depends Upon the ‘Exokine’ IL35
W. J. Burlingham¹, J. A. Sullivan¹, Y. Tomita², E. Jankowska-Gan¹, D. Lema¹, S. Hong³, D. A. Vignali⁴
¹Surgery, University of Wisconsin, Madison, WI, ²Surgery, Tokai University, Tokyo, Japan, ³Pharmacy, University of Wisconsin, Madison, WI, ⁴Immunology, University of Pittsburgh, Pittsburgh, PA
*Purpose: Interleukin-35 (IL35) is an immunosuppressive cytokine composed of Ebi3 and p35 subunits. Yet the form(s) that IL35 assumes and its role in infectious tolerance,…