Articles tagged "Alloantigens"

2017 American Transplant Congress
Modification of Human Endothelial Cells by CRISPR/Cas9-Mediated Ablation of Class I and II Major Histocompatibility Complex Antigen Expression.
J. Merola, C. Anderson, H. Xu, T. Manes, J. Pober.
Yale School of Medicine, New Haven, CT
Human endothelial cells (ECs) derived from cord blood progenitors have significant replicative potential and are thus well suited for use in tissue engineering of vascularized…
2017 American Transplant Congress
IdeS, an IgG Endopeptidase, Effectively Cleaves Anti-HLA Antibody (Ab), Resulting in Inhibition of Anti-HLA Ab-Mediated NK Cell Activation and Ab-Dependent Cell-Mediated Cytotoxicity (ADCC) in HLA-Sensitized Kidney Transplant Patients (HS KTx Pts).
S. Ge, M. Chu, J. Choi, S. Louie, A. Kang, A. Vo, A. Peng, S. Jordan, M. Toyoda.
Transplant Immunology Laboratory and Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA
Background: Ab-mediated rejection (ABMR) is a major obstacle in HS KTx Pts. ADCC is a mechanism responsible for ABMR. IdeS cleaves all 4 human IgG…
2017 American Transplant Congress
C4d Immunohistochemistry in Lung Allograft Interstitial Capillaries Lacks Linear Correlation with Donor Specific Antibodies, yet It Is Still Diagnostically Relevant.
Y. Butt,¹ T. Gokaslan,¹ V. Kaza,² F. Torres,² J. Torrealba.¹
¹Pathology, University of Texas Southwestern Medical Center, Dallas, TX; ²Lung Transplant Program, University of Texas Southwestern Medical Center, Dallas, TX
Antibody mediated rejection in lung allograft biopsies is not well defined from a morphologic or immunohistochemical aspect. Many centers routinely run immunohistochemistry for C4d as…
2016 American Transplant Congress
Natural Killer Cells Display Functional and Receptor Diversity in Response to Alloantigen.
O. Hatton,¹ T. Shawler,¹ C. Blish,² O. Martinez,¹ S. Krams.¹
¹Division of Abdominal Transplantation, Dept. of Surgery, Stanford University, Stanford; ²Dept of Medicine, Stanford University, Stanford.
Natural killer (NK) cells play an important role in the first-line defense against tumor and virus-infected cells. Although “missing-self” suggests NK cells would target foreign…
2016 American Transplant Congress
Ex Vivo Expansion and Functional Characterization of Human Alloantigen-Specific Regulatory T Cells.
J. Mathew,¹ S. McEwen,¹ I. Konieczna,¹ X. Huang,¹ J. He,¹ R. Kornbluth,² J. Miller,¹ L. Gallon,¹ J. Leventhal.¹
¹Surgery - Comprehensive Transplant Center, Northwestern University, Chicago, IL; ²Multimeric Biotherapeutics, Inc, La Jolla, CA.
Purpose: Regulatory T cells (Tregs) have been associated with clinical transplant tolerance and they have been shown to prolong graft survival in animal models without…
2016 American Transplant Congress
Alemtuzumab Is Safe and Effective Induction Therapy in Highly Sensitized (HS) Pediatric Patients Undergoing Transplantation.
I. Kim,¹ J. Choi,¹ A. Vo,¹ A. Kang,¹ M. Toyoda,¹ J. Mirocha,² E. Kamil,¹ S. Louie,¹ O. Galera,¹ S. Jordan,¹ D. Puliyanda.¹
¹Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA; ²Biostatistics Core, Cedars-Sinai Medical Center, Los Angeles, CA.
Introduction: Studies show that alemtuzumab, a potent lymphocyte-depleting agent, is well-tolerated in pediatric renal transplantation. We report on the use of alemtuzumab induction in HS…
2016 American Transplant Congress
The Distance Between Us: The Landscape of Recipient-Specific Loss-of-Function in Solid Organ Transplantation and Association with Rejection-Free Graft Survival.
B. Cole,^1,2,5 J. van Setten,^3,5 B. Keating.^1,4,5
¹Department of Surgery, The Penn Transplant Institute, Philadelphia, PA; ²Center for Clinical Epidemiology and Biostatistics, The Institute for Biomedical Informatics, Philadelphia, PA; ³Department of Medical Genetics, University Medical Center Utrecht, Utrecht, Netherlands; ⁴Department of Surgery, Division of Transplant, the Children's Hospital of Philadelphia, Philadelphia, PA; ⁵The Omics Working Group, iGeneTRAiN Consortium, Philadelphia, PA.
Purpose: This study aims to comprehensively identify genes that are inactivated in both copies in transplant recipients but not in their respective donors, uncovering a…
2016 American Transplant Congress
Tracking of Endogenous Graft-Reactive Tregs Reveals Their Peripheral Enrichment During Transplantation Tolerance.
J. Young,¹ J. Chen,¹ M. Miller,¹ D. Yin,¹ J. Moon,² M.-L. Alegre,¹ A. Chong.¹
¹Surgery and Medicine, University of Chicago, Chicago, IL; ²Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, MA.
Purpose: Our goal for this study was to determine the relative fates of endogenous Foxp3- conventional (Tconv) and Foxp3+ regulatory T cells (Tregs) specific for…
2016 American Transplant Congress
Role of Regulatory T Cells in Induction of Tolerance in Mouse Vascularized Osteomyocutaneous Allotransplantation Under Combined Costimulation Blockade.
M. Anggelia,¹ H.-Y. Cheng,¹ Y.-L. Wang,¹ W.-Y. Chuang,¹ C.-H. Lin,¹ G. Brandacher,² F.-C. Wei,¹ C.-H. Lin.¹
¹Center for Vascularized Composite Allotransplantation, Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Linkou, Taoyuan, Taiwan; ²Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD.
Background: The role of regulatory T cells (Tregs) in allograft tolerance has been studied using various transplant models, but the mechanism in vascularized composite allotransplantation…
2016 American Transplant Congress
Profiling Metabolic Differences Between Graft- vs. Pathogen-Elicited CD8⁺ T Cell Responses.
A. Karp, M. Wagener, J. Robertson, M. Ford.
Emory University, Atlanta.
The challenge of immune modulation in transplantation is to inhibit pathologic alloreactivity while minimizing off-target toxicities and preserving protective immunity. Data have emerged demonstrating the…