Transplantation of Islet Preconditioned with Hydrogen Sulfide Enhances the Graft Function in Diabetic Mice.
Department of Renal Transplant, Center of Nephropathy, the First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an, China.
Meeting: 2016 American Transplant Congress
Abstract number: D83
Keywords: Drug interaction, Insulin, Pancreas transplantation, Survival
Session Information
Date: Tuesday, June 14, 2016
Session Name: Poster Session D: Chimerism/Stem Cells, Cellular/Islet Transplantation, Innate Immunity, Chronic Rejection
Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Halls C&D
Background: Islet transplantation is a promising therapy for Type 1 diabetes. However, successful outcomes are hampered by the poor survival of the donor cells after transplantation. Hydrogen sulfide (H2S), one gaseous signaling molecule, has been applied to inhibit cell apoptosis and promote cell survival. In the present study, we investigated whether H2S protective the survival of islets in vivo and in vitro.Methods:The viability of pre-treated islets with or without NaHS (as the donor of H2S) was tested by AO/PI staining, and the effect of islets was investigated by measuring glucose-stimulated insulin secretion in vitro. The microvascula density was determined by immunohistochemistry. Pre-treated or control islets were transplanted under the kidney capsule of diabetic mice. Graft function was investigated by monitoring blood glucose concentrations and mean survival time (MST).Results:The islet viability in pre-treated (94.7±0.5 %) group was higher than control group (90.1±0.8 %), which indicated that NaHS was important for islet survival during isolation. Furthermore, the response to glucose stimulation in pre-treated islets is superior to the control, the stimulation index was significantly higher (2.41±0.21vs1.52±0.25, p<0.01), which confirmed that NaHS improved islet function in vitro. The microvascular density of pre-treated islets was similar with untreated islets (9.23vs9.18, P<0.05). After transplantation under the kidney capsule, the MST of pre-treated islets was significantly longer than control islets (p<0.01). And pre-treated islets leaded to superior glucose control when compared with control islets (p<0.05), which confirmed that NaHS improved islet function in vivo.Conclusions: These results suggested that preconditioning islets with H2S effectively promotes islets survival and function in diabetic mice.
CITATION INFORMATION: Wang D, Ding M, Xue J, Zheng J, Feng S, Tian H, Li Y. Transplantation of Islet Preconditioned with Hydrogen Sulfide Enhances the Graft Function in Diabetic Mice. Am J Transplant. 2016;16 (suppl 3).
To cite this abstract in AMA style:
Wang D, Ding M, Xue J, Zheng J, Feng S, Tian H, Li Y. Transplantation of Islet Preconditioned with Hydrogen Sulfide Enhances the Graft Function in Diabetic Mice. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/transplantation-of-islet-preconditioned-with-hydrogen-sulfide-enhances-the-graft-function-in-diabetic-mice/. Accessed October 25, 2020.« Back to 2016 American Transplant Congress