Tissue Distribution, Preparation and Phenotype Characteristics of Stem Cells Originated from the Organs of Deceased Human Individuals
1Institute of Immunology and Experimental Therapy Polish Academy of Sciences, Wroclaw, Poland
2Department of Forensic Medicine, Medical University, Wroclaw, Poland.
Meeting: 2015 American Transplant Congress
Abstract number: B50
Keywords: Bone marrow, Cadaveric organs, Stem cells, Tissue-specific
Session Information
Session Name: Poster Session B: Cell Transplantation and Cell Therapies
Session Type: Poster Session
Date: Sunday, May 3, 2015
Session Time: 5:30pm-6:30pm
Presentation Time: 5:30pm-6:30pm
Location: Exhibit Hall E
Objective: Multipotent stem cells are unique cells present in several human organs that have self-renewing properties and are able to differentiate into diverse specialized cell types. The aim of this study was to verify wheatear it is possible to propagate in vitro, from different human tissues obtained from deceased donors, a population of cells that represent stem cells, and to characterize biologic properties of these cells.
Methods: Tissue samples were collected from 12 deceased donors with approval by the local Bioethics Committee. Samples of bone marrow (BM), skeletal muscle, heart, liver, and skin were harvested from12,5h to 48h after dead. Mononuclear cells from BM were isolated in the ficoll gradient. After tissue enzymatic digestion isolated cells were cultured from 31 to 59 days in standard DMEM medium (tissues) or in alpha MEM medium (BM) at 5%CO2. Tissue distribution and phenotype of cultured cells were characterized by immunostaining for: CD45, CD34, CD117, CD56, PAX7, CD73, CD90, CD105.
Results: Common feature of tissue localized stem cells was expression of c-kit (CD117), CD34 and CD56. Skeletal muscle progenitor cells exclusively express transcriptional factor PAX7. CD73 and CD90 were express on single stem cells of examined tissues and were localized in specific tissue compartments. We obtained live adherent cells after culture of BM, skeletal muscle and skin progenitor cells. After cell expansion BM-, muscle- and skin-derived live adherent cells exhibit similar phenotype (CD90+, CD73+, CD105+) characteristic for naïve mesenchymal stem cells (MSC). Muscle progenitor cells (but not from BM or skin) after culture additionally expressed CD56, and this suggest that those population of MSC is muscle-specific. All cultured cells of tissues-origin were negative for hematopoietic markers CD45, CD34 and CD117.
Conclusions: Stem cells with naïve MSC phenotype can be generated in vitro from several human tissues up to 48h after dead. The isolation of progenitor cells from human tissues and their expansion raises the possibility to obtain a population of stem cells capable of generating cells with naïve MSC phenotype. Expansion of tissue-origin MSC from deceased donor might have important clinical alternative in regenerative medicine.
Supported by NCN grant NN407121940
To cite this abstract in AMA style:
Klimczak A, Jurek T, Czuba M, Kozlowska U, Rorat M, Wrzeszcz K. Tissue Distribution, Preparation and Phenotype Characteristics of Stem Cells Originated from the Organs of Deceased Human Individuals [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/tissue-distribution-preparation-and-phenotype-characteristics-of-stem-cells-originated-from-the-organs-of-deceased-human-individuals/. Accessed May 9, 2025.« Back to 2015 American Transplant Congress