Objective This study was designed to retrospectively compare the effect of Serva NB1 collagenase between Vitacyte collagenase on islet isolation of DCD pancreas in China. Methods All the human pancreases were obtained from DCD donators at Tianjin First Center Hospital in China. For islet isolation procedure, the pancreas was trimmed and distended with Serva NB1 collagenase (Serva NB1, n=12) and Vitacyte collagenase (Vitacyte, n=5) and digested using a modified Ricordi semi-automatic method, the digested tissues were then collected and washed followed by the continuous density purification using the UIC-UB gradient in a Cobe 2991 cell separator. The evaluation of the islets quantity and quality was determined by digestion efficacy, islet yields, purity, viability and glucose-stimulated insulin release. Results Islet yields were similar between groups (ServaNB1: 438579.8±221132.8IEQ vs. Vitacyte: 442941.5±335943.4IEq, p>0.05). No significant purity differences were observed for fractions with purities greater than 75%. Viability was similar between the two groups (Serva NB1: 92.0%±5.02% vs. Vitacyte: 90.8%±3.22%, p>0.05) with comparable GSI (Serva NB1: 2.29±1.21 vs. Vitacyte: 1.93±0.72, p>0.05). However, we were intrigued to find that the digestion time in the Vitacyte group was significantly less than that in the Serva NB1 group (7.08±0.72min vs. 11.67±3.42min, p<0.05). The impact of cold ischemia and age on the isolation outcome in the Serva NB1 group was comparable to the Vitacyte group. ConclusionConclusively, Both Serva NB1 collagenase and Vitacyte collagenase can used for the islet isolation with Comparable outcomes from DCD pancreas in China.

CITATION INFORMATION: Liu Y, Sun P, Zou J, Wang G, Liu T, Wang L, Wang S. Serva NB1 versus Vitacyte Collagenase: Comparable Human Islet Isolation Outcomes Using DCD Donor Pancreas in China. Am J Transplant. 2017;17 (suppl 3).

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