Background: Renal ischemia reperfusion (I/R) injury is the main factors to affect the early functional recovery and the long-term survival of transplanted kidney in renal transplantation. We have found that NKA DR region specific antibody(DRSAb) could induce protective effect on HK-2 cells against I/R injury via PI3K/Akt and PKCΕ signal pathway. Based on the findings, this experimental study was designed to investigate protective effet of DRSAb against I/R injury on kidney in rats.
Methods: All studies were conforming to the 1991 revision of "Guiding Principles in the Care and Use of Animals" (American Physiological Association). Fifty Sprague Dawley rats were randomly divided into 5 groups including a control group and four I/R groups. Three I/R groups were treated by DRSAb(5mg/kg,i.p.) at three different time point (before ischemia, during ischemia and late reperfusion). The histopathological findings including apoptotic changes, and also tissue malondialdehyde (MDA), superoxide dismutase (SOD), blood urea nitrogen (BUN) and serum creatinine (Cr) levels were determined. Western blot analysis was employed to study the cell signaling mechanisms.
Results: The renal I/R model data showed that kidney tissue MDA levels were found to be significantly higher in the I/R group, whereas the values of SOD did not reach to statistical meaning level in I/R group. DRSAb given before and during ischemia reduced the elevated MDA levels to the nearly control levels. Serum levels of BUN and Cr were significantly higher in I/R group. DRSAb given before and during ischemia significantly reduced the elevated BUN and Cr levels when compared to I/R group. Renal I/R injury also induced extensive tubular necrosis, glomerular damage and apoptosis in the histological evaluation. DRSAb ameliorated these histological damages in all treatment groups. Western blotting analysis further confirmed that the phosphorylated PI3K/Akt and PKCΕ levels in the tissue of DRSAb treated groups were significantly higher than control and I/R group.
Conclusion: In this study, the protective effects of DRSAb against renal I/R injury has been evaluated for the first time. And this protective effect may via stimulation of PI3K/Akt and PKCΕ pathways.
To cite this abstract in AMA style:Zheng J, Xue W, Tian P, Ding X, Yan H, Hou J, Tian X, Li Y, Ding C. Protective Effect and Mechanism of NKA DR Region Specific Antibody on Ischemia-Reperfusion-Induced Renal Injury in Rats [abstract]. Am J Transplant. 2013; 13 (suppl 5). https://atcmeetingabstracts.com/abstract/protective-effect-and-mechanism-of-nka-dr-region-specific-antibody-on-ischemia-reperfusion-induced-renal-injury-in-rats/. Accessed April 4, 2020.
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