Abstracts Archive - Page 321 of 1683 - ATC Abstracts

2021 American Transplant Congress
Cold Perfusion for Ischemia Minimization Reduces Cardiac Injury in an Ex Vivo Xenoperfusion Model
M. R. Connolly¹, A. Calhoun¹, F. Pollok¹, L. Burdorf¹, Z. Habibabady¹, M. Ma¹, S. Miura¹, W. Eyestone², C. Phelps², D. Ayares²
¹Massachusetts General Hospital, Boston, MA, ²Revivicor, Blacksburg, VA
*Purpose: Prior studies using Steen’s cold perfusion storage technique for heart ischemia minimization (IM) prevented initial xenograft dysfunction (IXD). Our pilot data evaluates the effect…
2021 American Transplant Congress
Donor Lymphocytes in Peripheral Blood of Patients After Lung Transplantation Comprise High Frequencies of Killer Cell Immunoglobulin-like Receptor-positive T and NK Cell Subsets
J. F. Kühne¹, A. Hitz¹, K. A. Bläsing¹, B. Wiegmann², R. Bellmàs Sanz¹, F. Ius², A. Haverich², G. Warnecke², C. S. Falk¹
¹MHH, Institute of Transplant Immunology, Hannover, Germany, ²MHH, Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover, Germany
*Purpose: For end-stage lung diseases, lung transplantation (LuTx) is the only curative treatment option. However, acute and chronic rejections are major limitations. Thus, a deeper…
2021 American Transplant Congress
Imaging Flow Cytometry Reveals That Platelet-Sized Erythrocyte Fragments are Formed During Pig Organ Perfusion with Human Blood
Z. A. Habibabady¹, F. Ellett², L. Burdorf¹, M. Connolly¹, F. Pollok¹, D. Irimia², R. N. Pierson III¹, A. M. Azimzadeh¹
¹Center for Transplantation Sciences, Massachusetts General Hospital and Harvard School of Medicine, Boston, MA, ²Center for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard School of Medicine, Boston, MA
*Purpose: Severe thrombocytopenia frequently occurs after xenotransplantation of porcine cells or organs, and when pig organs are perfused with human blood. We developed a flow…
2021 American Transplant Congress
Estrogen Receptors Alpha and Beta are Required for Immune Homeostasis and Normal Treg Function
L. M. Christensen¹, T. Akimova², G. Ge³, R. Han¹, L. Wang¹, P. Hernandez³, C. O'Brien³, M. H. Levine³, W. W. Hancock¹
¹Children's Hospital of Philadelphia, Philadelphia, PA, ²Children's Hospital of Philadelphia and University of Pennsylvania, Philadelphia, PA, ³University of Pennsylvania, Philadelphia, PA
*Purpose: Estrogen signaling is a critical component of mammalian homeostasis, acting through a complex and interconnected system of hormone synthesis pathways and receptor signaling cascades.…
2021 American Transplant Congress
The Co-Occurrence of Newly Developed HLA Donor Specific Antibody and Positive C3d/C4d Profiles Along with Immune Therapy Non-Compliance in Pediatric Heart Transplant Recipients is a High Risk Indicator for Patient Mortality
A. Zhang, C. Nasman, J. Allen, Y. Sun, D. Thomas, R. Rodriguez, C. Tan, G. Boyle
Cleveland Clinic Foundation Transplant Center, Cleveland, OH
*Purpose: Increasing evidence has implicated the deleterious effect of HLA donor specific antibody (DSA) in pediatric heart transplantation (PHTx). However, there are still insufficient research…
2021 American Transplant Congress
Triple-Knockout Pig Red Blood Cells Are a Potential Alternative Source for Clinical Blood Transfusion
T. Yamamoto¹, M. H. Bikhet¹, H. Q. Nguyen¹, M. Javed¹, D. Ayares², H. Iwase¹, H. Hara¹, D. K. Cooper¹
¹Xenotransplantation Program, Surgery, The University of Alabama at Birmingham, Birmingham, AL, ²Revivicor, Blacksburg, VA
*Purpose: Triple-knockout (TKO) pig RBCs (pRBCs) may be an alternative source for clinical blood transfusion because almost humans have no preformed antibody to TKO pRBCs.…
2021 American Transplant Congress
Transgenic Overexpression of Human Ectonucleoside Triphosphate Diphosphohydrolase 1 (entpd1, Or Hcd39) in Pig to Primate Xenotransplantation Models
R. Matheson¹, K. Deng¹, K. M. Lee¹, Z. A. Habibabady¹, C. G. Rickert¹, K. J. Ahrens¹, J. M. O¹, D. Becerra¹, P. Patel¹, W. Westlin², M. Youd², N. Serifis¹, T. M. Coe¹, D. Cloonan¹, A. M. Azimzadeh¹, C. LeGuern¹, J. C. Madsen¹, S. C. Robson³, J. F. Markmann¹
¹Transplantation Surgery Research, Massachusetts General Hospital, Boston, MA, ²eGenesis, Cambridge, MA, ³Beth Israel Deaconess Medical Center, Boston, MA
*Purpose: Human CD39 (hCD39) is the gene of interest in xenotransplantation due to its reported capacity to prevent platelet aggregation by metabolizing extracellular ATP to…
2021 American Transplant Congress
HTFPI.hCD47 and Adhesion Inhibition in GTKO.hCD46 Pig Lung Xenograft Injury
S. Miura¹, L. Burdorf¹, Z. Habibabady¹, A. Dandro², M. Connolly¹, S. Pratts¹, C. Phelps², W. Eyestone², D. Ayares², A. Azimzadeh¹, R. Pierson III¹
¹Center for Transplantation Sciences, Massachusetts General Hospital, Charlestown, MA, ²Revivicor, Inc, Blacksburg, VA
*Purpose: We evaluate whether genetic expression of human tissue factor pathway inhibitor (TFPI, extrinsic coagulation pathway inhibitor) and human CD47 ("don’t eat me" immunoregulator), alone…
2021 American Transplant Congress
Sampling Renal Biopsies in Pre-Clinical Research for Comprehensive Assessment
C. M. Edwards¹, C. Albert², D. Stern¹, J. Langford¹, W. Day², J. R. DiRito¹, W. M. Saltzman², M. Kashgarian³, J. S. Pober⁴, G. T. Tietjen¹
¹Surgery, Yale School of Medicine, New Haven, CT, ²Biomedical Engineering, Yale School of Medicine, New Haven, CT, ³Pathology, Yale School of Medicine, New Haven, CT, ⁴Immunobiology, Yale School of Medicine, New Haven, CT
*Purpose: Previous literature has demonstrated that single biopsies are not representative of a whole kidney in clinical assessments. In this study we aimed to design…
2021 American Transplant Congress
MiRNA as Regulator of Cell Death and Inflammatory Response in Hepatic Iri
E. Bardhi¹, T. Rousselle¹, J. McDaniels¹, S. Bontha¹, J. Eason², D. Maluf¹, V. Mas³
¹Surgery, University of Maryland, Baltimore, MD, ²Surgery, James D Eason Transplant Institute, Memphis, TN, ³Surgery, University of Maryland - Division of Surgical Science, Baltimore, MD
*Purpose: Hepatic IRI represents a barrier to the use of available organs. Early graft dysfunction of donor livers is directly related to the severity of…