Session Time: 5:30pm-6:30pm
Presentation Time: 5:30pm-6:30pm
Location: Exhibit Hall E
To investigate the relationship between early-stage renal acute rejection and the level of BCA-1 in urine, explore the diagnostic value and noninvasive monitoring in early stage after transplantation by measurement of urine BCA-1.
155 patients were enrolled, including 49 with biopsy-proved acute rejection, 58 patients with stable renal function and no abnormal histological findings, 9 patients with biopsy-proven acute tubular necrosis and 29 patients with biopsy-proven chronic allograft nephropathy. Additionally, urinary samples were also collected from 40 healthy controls. BCA-1 was measured in urinary samples using a commercial human BCA-1 enzyme-linked immunosorbent assay (ELISA) kit ((R&D Systems). Statistical analysis was performed by using SPSS software package. (Version 16.0) .Results were expressed in the text as mean ± SEM unless otherwise stated. P<0.05 was considered statistically significant. BCA-1 levels were expressed per micromole of urinary creatinine to correct for difference in urinary concentration.
Patient with acute rejection excreted urinary BCA-1 at a significantly higher level (8.1±2.1 pg/¯o;mol creatine) than levels of patients with No-AR and healthy controls (P<0.001). Patients with acute tubular necrosis excreted urinary BCA-1 at a significantly lower level(2.52±0.57 pg/¯o;mol creatinine)than levels of patients with acute rejection. Patients with steroid-resistant acute rejection had significantly greater urinary BCA-1 concentration than patients with steroid-sensitive acute rejection (11±2.82 pg/¯o;mol creatinine vs 5.34±4.28pg/¯o;mol creatinine, P=0.0032). Patients with acute humoral rejection had significantly higher urinary BCA-1 concentration than patients with acute celluar rejection (24.2±6.68, pg/¯o;mol creatinine vs 2.91±0.65pg/¯o;mol creatinine, P=0.0002). ROC curve was constructed to determine the discriminatory power of BCA-1 levels for diagnosis of acute humoral rejection. The area under ROC curve was 0.867 (95%CI: 0.725-1.005), which showed that BCA-1 was a suitable marker for the diagnosis of acute humoral rejection. At a cut point of 8.3 pg/¯o;mol creatine, the sensitivity was 75% and the specificity was 91.9% .
To cite this abstract in AMA style:Chen D, Chen J, Peng W. Noninvasive Diagnostic and Predictive Value in Renal Transplant Recipients by Measurement of Urine BCA-1 [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/noninvasive-diagnostic-and-predictive-value-in-renal-transplant-recipients-by-measurement-of-urine-bca-1/. Accessed May 5, 2021.
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