Noninvasive Diagnostic and Predictive Value in Renal Transplant Recipients by Measurement of Urine BCA-1
Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Key Laboratory of Nephropathy, Zhejiang Province
Kidney Disease Immunology Laboratory, The Third Grade Laboratory, State Administration of Traditional Chinese.
Meeting: 2015 American Transplant Congress
Abstract number: D279
Keywords: Kidney transplantation, Rejection
Session Information
Session Name: Poster Session D: Late Breaking
Session Type: Poster Session
Date: Tuesday, May 5, 2015
Session Time: 5:30pm-6:30pm
Presentation Time: 5:30pm-6:30pm
Location: Exhibit Hall E
Objective
To investigate the relationship between early-stage renal acute rejection and the level of BCA-1 in urine, explore the diagnostic value and noninvasive monitoring in early stage after transplantation by measurement of urine BCA-1.
Methods
155 patients were enrolled, including 49 with biopsy-proved acute rejection, 58 patients with stable renal function and no abnormal histological findings, 9 patients with biopsy-proven acute tubular necrosis and 29 patients with biopsy-proven chronic allograft nephropathy. Additionally, urinary samples were also collected from 40 healthy controls. BCA-1 was measured in urinary samples using a commercial human BCA-1 enzyme-linked immunosorbent assay (ELISA) kit ((R&D Systems). Statistical analysis was performed by using SPSS software package. (Version 16.0) .Results were expressed in the text as mean ± SEM unless otherwise stated. P<0.05 was considered statistically significant. BCA-1 levels were expressed per micromole of urinary creatinine to correct for difference in urinary concentration.
Results
Patient with acute rejection excreted urinary BCA-1 at a significantly higher level (8.1±2.1 pg/¯o;mol creatine) than levels of patients with No-AR and healthy controls (P<0.001). Patients with acute tubular necrosis excreted urinary BCA-1 at a significantly lower level(2.52±0.57 pg/¯o;mol creatinine)than levels of patients with acute rejection. Patients with steroid-resistant acute rejection had significantly greater urinary BCA-1 concentration than patients with steroid-sensitive acute rejection (11±2.82 pg/¯o;mol creatinine vs 5.34±4.28pg/¯o;mol creatinine, P=0.0032). Patients with acute humoral rejection had significantly higher urinary BCA-1 concentration than patients with acute celluar rejection (24.2±6.68, pg/¯o;mol creatinine vs 2.91±0.65pg/¯o;mol creatinine, P=0.0002). ROC curve was constructed to determine the discriminatory power of BCA-1 levels for diagnosis of acute humoral rejection. The area under ROC curve was 0.867 (95%CI: 0.725-1.005), which showed that BCA-1 was a suitable marker for the diagnosis of acute humoral rejection. At a cut point of 8.3 pg/¯o;mol creatine, the sensitivity was 75% and the specificity was 91.9% .
To cite this abstract in AMA style:
Chen D, Chen J, Peng W. Noninvasive Diagnostic and Predictive Value in Renal Transplant Recipients by Measurement of Urine BCA-1 [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/noninvasive-diagnostic-and-predictive-value-in-renal-transplant-recipients-by-measurement-of-urine-bca-1/. Accessed May 17, 2025.« Back to 2015 American Transplant Congress