Membrane Proteome Profiling Reveals Molecular Changes Associated with Donor-Specific Tolerance to Vascularized Composite Allotransplants.
1Center for Vascularized Composite Allotransplantation, Chang Gung Memorial Hospital, Gueishan, Taoyuan, Taiwan
2Master Program for Pharmacogenomics and Pharmacoproteomics, Taipei Medical University, Taipei, Taiwan
3Institute of Chemistry, Academia Sinica, Taipei, Taiwan
4College of Medicine, Chang Gung University, Gueishan, Taoyuan, Taiwan.
Meeting: 2016 American Transplant Congress
Abstract number: A99
Keywords: Tolerance
Session Information
Session Name: Poster Session A: Clinical Vascularized Composite Allotransplantation
Session Type: Poster Session
Date: Saturday, June 11, 2016
Session Time: 5:30pm-7:30pm
Presentation Time: 5:30pm-7:30pm
Location: Halls C&D
Background: although donor-specific tolerance to vascularized composite allotransplants (VCAs) has been successfully induced with different regimes, molecular changes associated with tolerance are not well understood.
Materials and Methods: spleens from naïve LEW and allogeneic VCA (BN as the donor and LEW as the recipient) recipients that have developed donor-specific tolerance were collected. As many cellular communications occur on membrane, herein membrane proteins were specifically isolated for further analysis. Proteins were then labeled with isobaric tagging (iTRAQ) followed by LC-MS/MS for quantitative proteomics analysis. Proteins that were up- or down-regulated at the tolerant recipients compared to naïve rats were further characterized.
Results and Discussion: around 1900 proteins were identified and ~1700 of them were quantified. About 100 proteins showed significant changes between tolerant and naïve samples. Several proteins participating in T cell receptor signaling, such as CD8, CD28, and CD3 were shown to down-regulate at the tolerant samples, corroborating our previous data that the level of CD8 in peripheral blood was significantly dropped at the recipients developed tolerance (1), and the feasibility of this methodology was thus verified. Other identified proteins included protein kinases, chemokine receptors, protease inhibitors, and respiratory chain proteins, to name a few. Ongoing efforts are to characterize the mechanistic roles of these proteins, as well as their potentials to serve as biosignature for VCA tolerance.
Reference:
1. Ramirez AE, Cheng HY, Lao WW, Wang YL, Wen CJ, Wallace CG, et al. A novel rat full-thickness hemi-abdominal wall/hindlimb osteomyocutaneous combined flap: influence of allograft mass and vascularized bone marrow content on vascularized composite allograft survival. Transpl Int 2014; 27: 977.
CITATION INFORMATION: Cheng H.-Y, Han C.-L, Jiang F.-W, Shih L.-Y, Lin C.-F, Chen Y.-J, Wei F.-C. Membrane Proteome Profiling Reveals Molecular Changes Associated with Donor-Specific Tolerance to Vascularized Composite Allotransplants. Am J Transplant. 2016;16 (suppl 3).
To cite this abstract in AMA style:
Cheng H-Y, Han C-L, Jiang F-W, Shih L-Y, Lin C-F, Chen Y-J, Wei F-C. Membrane Proteome Profiling Reveals Molecular Changes Associated with Donor-Specific Tolerance to Vascularized Composite Allotransplants. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/membrane-proteome-profiling-reveals-molecular-changes-associated-with-donor-specific-tolerance-to-vascularized-composite-allotransplants/. Accessed May 10, 2025.« Back to 2016 American Transplant Congress