ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Influence of Regulatory B Cells on B Cells

Q. Fu1, K. M. Lee2, K. Deng2, D. Agarwal1, R. Matheson3, T. Press1, J. Lei4, H. Yang5, C. LeGuern2, S. Deng5, J. F. Markmann2

1Center for Transplant Science, MGH, Boston, MA, 2Massachusetts General Hospital, Boston, MA, 3Transplantation Surgery Research, Massachusetts General Hospital, Boston, MA, 4Surgery, Mass General, Boston, MA, 5Sichuan Provincial People's Hospital, University of Electronic Sciences and Technology of China, Chengdu, China

Meeting: 2022 American Transplant Congress

Abstract number: 1229

Keywords: B cells

Topic: Basic Science » Basic Science » 04 - B-cell / Antibody /Autoimmunity

Session Information

Session Name: B-cell / Antibody /Autoimmunity

Session Type: Poster Abstract

Date: Monday, June 6, 2022

Session Time: 7:00pm-8:00pm

 Presentation Time: 7:00pm-8:00pm

Location: Hynes Halls C & D

*Purpose: Our previous study showed that Bregs-TLR, one type of previously studied Breg expanded in vitro, are capable of suppressing naïve B cell proliferation in the presence of LPS stimulation. Our previous result showed Bregs-TLR rescuing more LPS-stimulated B cells from apoptosis in vitro. However, less is known about how Bregs-TLR influence B cell activation and differentiation when LPS is absent. In the current study, we aim to explore the rescuing role of Bregs-TLR on naive B cells.

*Methods: Bregs-TLR were obtained after purified splenic B6 B cells were given CpG ODN1668 (TLR9 agonist) stimulation for 3 days and LPS (10ug/ml), PMA (50ng/ml), and ionomycin (1ug/ml) stimulation for the last 5 hours. In vitro, Bregs-TLR and naïve B cells w/o LPS stimulation were co-cultured for 3 days. In vivo, B6 recipients were adoptively transferred with Bregs-TLR on D-1 and transplanted with OVA skin grafts on D0. B cell subsets and markers of proliferation were measured by flow cytometry on day 21.

*Results: Flow cytometry found that naive B cells showed low viability and little proliferation in comparison to B cells stimulated for 96h with LPS. In the complete absence of any LPS stimulation, B cells did not survive unless Bregs-TLR or Bregs-CpG were added. Interestingly, while Bregs-CpG induced five generations of B cell proliferation, Bregs-TLR induced a comparable amount of proliferation in fewer generations (5 vs. 2). On the other hand, in vivo data showed that Bregs-TLR could induce B cell proliferation (Figure) and more CD19+IgMhiIgDloCD23loCD21hi MZ B cells in B6 recipients while Bregs-CpG could not.

*Conclusions: Bregs-TLR regulate the proliferation and differentiation of naïve B cells both in vitro and in vivo. The increase of MZ B cell percentage may indicate that the mechanism for which Bregs-TLR induce the proliferation of other B cells lies in an MZ B cell-relevant pathway.

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Fu Q, Lee KM, Deng K, Agarwal D, Matheson R, Press T, Lei J, Yang H, LeGuern C, Deng S, Markmann JF. Influence of Regulatory B Cells on B Cells [abstract]. Am J Transplant. 2022; 22 (suppl 3). https://atcmeetingabstracts.com/abstract/influence-of-regulatory-b-cells-on-b-cells/. Accessed May 9, 2025.

« Back to 2022 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences