Impact of complement component 3 single nuclear polymorphisms on renal transplant recipients with antibody-mediated rejection

Zijie Wang, Zhijian Han, Jun Tao, Ruoyun Tan, Min Gu

Department of Urology, Nanjing Medical University First Affiliated Hospital, Nanjing, China, 210029;

Abstract

Background: Antibody-mediated rejection (ABMR) is an important risk of allograft dysfunction in kidney transplantation. The complement 3 (C3) is considered to be associated with the generation of alloreative antibodies and donor-specific antibodies. However, the association of C3 single nuclear polymorphisms (SNPs) with ABMR still remained unclear.

Methods: Blood samples of 199 renal transplant recipients containing 68 with ABMR and 131 with stable graft function were collected, and analyzed by next-generation sequencing with an established gene panel. High quality readout was obtained in 15 SNPs.

Results: After being adjusted with age, sex and immunosuppressive protocols, rs10411506 and rs2230205 were found to be significantly associated with ABMR in dominant model (rs10411506: OR=2.73, 95% CIs: 1.16, 6.68, P=0.028; rs2230205: OR=2.52, 95% CIs: 1.07, 5.92, P=0.034); rs10411506, rs2230205 and rs2230201 were found statistically different in HET model (rs10411506: OR=3.05, 95% CIs: 1.22, 7.64, P=0.017; rs2230205: OR=2.90, 95% CIs: 1.20, 7.00, P=0.018; rs2230201: OR=2.41, 95% CIs: 1.03, 5.64, P=0.042). The linkage analysis showed relatively low linkage disequilibrium among these SNPs.

Conclusions: Our study firstly identified the two SNPs (rs10411506 and rs2230205) in C3 gene were significantly correlated with ABMR in kidney transplantation. These findings may have implications for the diagnosis and prevention of ABMR.

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CITATION INFORMATION: Wang Z, Han Z, Tao J, Tan R, Gu M. Impact of Complement Component 3 Single Nuclear Polymorphisms on Renal Transplant Recipients with Antibody-Mediated Rejection. Am J Transplant. 2017;17 (suppl 3).

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