Histone Methyltransferase EZH2 Was Involved in the Mechanism of let-7e Inhibition of TNF-α Expression.

J. Huang,^1,2,3 Y. Zhang,^1,2,3 Q. Zhang,^1,2,3 L. Gui,^1,2,3 Y. Cai,^1,2,3 X. Deng.^1,2,3

¹Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China
²Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation), The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China
³Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China

Meeting: 2017 American Transplant Congress

Abstract number: C127

Keywords: Tumor necrosis factor (TNF)

Session Information

Session Name: Poster Session C: Innate Immunity

Session Type: Poster Session

Date: Monday, May 1, 2017

Session Time: 6:00pm-7:00pm

Presentation Time: 6:00pm-7:00pm

Location: Hall D1

Inflammatory cytokines, such as TNF-α, play important roles in transplantation and infection. The expression of these inflammatory factors can be regulated by many kinds of epigenetic regulation factors including histone methyltransferase and microRNAs, but the regulatory mechanisms and the relationship between these regulatory factors remain unclear. Our previous studies showed that inflammation-related microRNA let-7e could target Enhancer Zeste 2 (EZH2), a histone methyltransferase catalyzing histone H3K27me3, and silence of EZH2 could inhibit TNF-α expression after dengue virus infection or LPS stimulation in monocytic cell line. In further studies we found siEZH2 lead to downregulation of Arfaptin2 and ARFGAP1, two vesicular transporter that are helpful for cytokine secretion with the function in ADP-ribosylation factor (Arf)-dependent membrane traffic outside the ER-Golgi. siEZH2 can also inhibit NF kappa B pathway to downregulate TNF-α expression.

Keywords: EZH2; let-7e; TNF α.

CITATION INFORMATION: Huang J, Zhang Y, Zhang Q, Gui L, Cai Y, Deng X. Histone Methyltransferase EZH2 Was Involved in the Mechanism of let-7e Inhibition of TNF-α Expression. Am J Transplant. 2017;17 (suppl 3).

To cite this abstract in AMA style:

Huang J, Zhang Y, Zhang Q, Gui L, Cai Y, Deng X. Histone Methyltransferase EZH2 Was Involved in the Mechanism of let-7e Inhibition of TNF-α Expression. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/histone-methyltransferase-ezh2-was-involved-in-the-mechanism-of-let-7e-inhibition-of-tnf-expression/. Accessed November 22, 2024.

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